DNA methylation-specific multiplex assays for body fluid identification.
Seoul, South Korea. In Int J Legal Med, 2013
After confirming the stability of the body fluid-specific DNA methylation profile over time, two different multiplex PCR systems were constructed using methylation-sensitive restriction enzyme PCR and methylation SNaPshot, in order to analyze the methylation status of specific CpG sites from the USP49, DACT1, PRMT2, and PFN3 tDMRs.
Potential forensic application of DNA methylation profiling to body fluid identification.
Seoul, South Korea. In Int J Legal Med, 2012
Five tDMRs for the genes DACT1, USP49, HOXA4, PFN3, and PRMT2 were selected, and DNA methylation profiles for these tDMRs were produced by bisulfite sequencing using pooled DNA from blood, saliva, semen, menstrual blood, and vaginal fluid.
A pipeline to determine RT-QPCR control genes for evolutionary studies: application to primate gene expression across multiple tissues.
Durham, United States. In Plos One, 2009
We identified 13 genes from the pipeline and from commonly used control genes: ACTB, USP49, ARGHGEF2, GSK3A, TBP, SDHA, EIF2B2, GPDH, YWHAZ, HPTR1, RPL13A, HMBS, and EEF2.