TAS-102, a novel antitumor agent: A review of the mechanism of action.
Los Angeles, United States. In Cancer Treat Rev, Nov 2015
TAS-102 is an oral combination therapy consisting of trifluridine (FTD), a thymidine-based nucleoside analog, plus tipiracil hydrochloride (TPI), a novel thymidine phosphorylase inhibitor that improves the bioavailability of FTD.
Enzyme dynamics from NMR spectroscopy.
New York City, United States. In Acc Chem Res, Mar 2015
This Account reviews NMR spin relaxation studies of the enzymes ribonuclease HI from mesophilic (Escherichia coli) and thermophilic (Thermus thermophilus) bacteria, E. coli AlkB, and Saccharomyces cerevisiae triosephosphate isomerase to illustrate the contributions of conformational flexibility and dynamics to diverse steps in enzyme mechanism.
Precision is essential for efficient catalysis in an evolved Kemp eliminase.
Zürich, Switzerland. In Nature, 2013
Starting from a computationally designed catalyst for the Kemp elimination--a well-studied model system for proton transfer from carbon--we show that an artificial enzyme can be evolved that accelerates an elementary chemical reaction 6 × 10(8)-fold, approaching the exceptional efficiency of highly optimized natural enzymes such as triosephosphate isomerase.
Perspectives in enzymology of membrane proteins by solid-state NMR.
Frankfurt am Main, Germany. In Acc Chem Res, 2013
Solid-state NMR has also provided a mechanistic understanding of soluble enzymes including triosephosphate isomerase (TIM) and different metal-binding proteins, which demonstrates a promising perspective also for membrane proteins.
Reappraisal of triosephosphate isomerase deficiency.
In Eur J Haematol, 2011
structural changes rather than abnormal catalysis may play an important role in the clinical manifestations of TPI deficiency; the postulated high aggregation ability of the unstable Glu104Asp mutant would lead to more serious symptoms