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Taste receptor, type 2, member 4

T2R4, hTAS2R4
This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: GPCR, ACID, hTAS2R16, TM3, SIMPLE
Papers on T2R4
The structure-function role of C-terminus in human bitter taste receptor T2R4 signaling.
Chelikani et al., Winnipeg, Canada. In Biochim Biophys Acta, Jul 2015
Using site-directed mutagenesis we studied the role of 16 residues in the C-terminus of T2R4.
Abscisic Acid Acts as a Blocker of the Bitter Taste G Protein-Coupled Receptor T2R4.
Chelikani et al., Winnipeg, Canada. In Biochemistry, May 2015
Recently, we elucidated the binding pocket of T2R4 for its agonist quinine, and an antagonist and inhibitory neurotransmitter, γ-aminobutyric acid.
Amino acid derivatives as bitter taste receptor (T2R) blockers.
Chelikani et al., Saskatoon, Canada. In J Biol Chem, 2014
In this study, using molecular modeling guided site-directed mutagenesis, we elucidated the ligand-binding pocket of T2R4.
Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells.
Chelikani et al., Winnipeg, Canada. In Biochem Biophys Res Commun, 2014
In this report using quantitative (q)-PCR and flow cytometry, we characterized the expression of T2R1, T2R4, T2R10, T2R38 and T2R49 in the highly metastatic breast cancer cell line MDA-MB-231, poorly metastatic cell line MCF-7, and non-cancerous mammary epithelial cell line MCF-10A.
The third intracellular loop plays a critical role in bitter taste receptor activation.
Chelikani et al., Winnipeg, Canada. In Biochim Biophys Acta, 2014
Here, we pursue an alanine scan mutagenesis of the ICL3 of T2R4 and characterize the functionality of 23 alanine mutants.
A biomimetic bitter receptor-based biosensor with high efficiency immobilization and purification using self-assembled aptamers.
Wang et al., Hangzhou, China. In Analyst, 2013
A human bitter receptor, T2R4, was expressed on the plasma membrane of HEK-293 cells and fused with a His6-tag on its C-terminal.
Rebaudioside A and Rebaudioside D bitterness do not covary with Acesulfame K bitterness or polymorphisms in TAS2R9 and TAS2R31.
Hayes et al., United States. In Chemosens Percept, 2013
RebA has shown to activate hTAS2R4 and hTAS2R14 in vitro.
Identification of novel genes for bitter taste receptors in sheep (Ovis aries).
Almeida et al., Évora, Portugal. In Animal, 2013
In sheep, we identified eight orthologs of cattle genes: T2R16, T2R10B, T2R12, T2R3, T2R4, T2R67, T2R13 and T2R5.
Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras.
Chelikani et al., Winnipeg, Canada. In Biochem Biophys Res Commun, 2013
In this study, we elucidated how different lengths of rhodopsin N-terminal sequence effect the structure and function of the bitter taste receptor, T2R4.
Constitutively active mutant gives novel insights into the mechanism of bitter taste receptor activation.
Chelikani et al., Winnipeg, Canada. In J Neurochem, 2012
We mutated Gly28¹·⁴⁶ and Ser285⁷·⁴⁷ in T2R4 to G28A, G28L, S285A, S285T, and S285P, and carried out pharmacological characterization of the mutants.
Human psychometric and taste receptor responses to steviol glycosides.
Hofmann et al., Freising, Germany. In J Agric Food Chem, 2012
A comprehensive screening of 25 human bitter taste receptors revealed that two receptors, hTAS2R4 and hTAS2R14, mediate the bitter off-taste of steviol glycosides.
Behavioral analysis of Drosophila transformants expressing human taste receptor genes in the gustatory receptor neurons.
Isono et al., Sendai, Japan. In J Neurogenet, 2012
Transgenic Drosophila expressing human T2R4 and T2R38 bitter-taste receptors or PKD2L1 sour-taste receptor in the fly gustatory receptor neurons and other tissues were prepared using conventional Gal4/UAS binary system.
Functional bitter taste receptors are expressed in brain cells.
Chelikani et al., Winnipeg, Canada. In Biochem Biophys Res Commun, 2011
RT-PCR analysis revealed the presence of T2R4, T2R107 and T2R38 transcripts in the brain stem, cerebellum, cortex and nucleus accumbens.
Bitter peptides activate hTAS2Rs, the human bitter receptors.
Huang et al., Tokyo, Japan. In Biochem Biophys Res Commun, 2008
HEK293 cells expressing hTAS2R1, hTAS2R4, hTAS2R14, and hTAS2R16 responded to bitter casein digests.
Functional expression of mammalian bitter taste receptors in Caenorhabditis elegans.
Andres-Barquin et al., Basel, Switzerland. In Biochimie, 2006
As a proof of the concept, we generated transgenic worms expressing human T2R4 or its mouse ortholog T2R8 receptors, which respond to two bitter tastants previously characterised as their functional ligands, 6-n-propyl-2-thiouracil and denatoniun.
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