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SPT16 Spt16p

Spt16, Cdc68, Spt16p
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009] (from NCBI)
Top mentioned proteins: FACT, Histone, POLYMERASE, CAN, H2A
Papers on Spt16
Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.
Ito et al., Kumamoto, Japan. In Febs Open Bio, 2013
A total of 253 phosphoproteins were identified, including FACT complex subunit SPT16 in the 1-day exposure group, keratin type 1 cytoskeletal 18 (K18), and adipocyte fatty acid-binding protein, in the 7-day exposure group, and peroxiredoxin-1 (OSF3) and spectrin β chain brain 1 (SPTBN1), in both groups.
A role for FACT in repopulation of nucleosomes at inducible genes.
Stillman et al., Boston, United States. In Plos One, 2013
Based on an interaction between Pdr1 and the FACT complex, we show that strains with spt16 or pob3 mutations are sensitive to xenobiotic drugs and display diminished PDR gene induction.
Enhanced chromatin dynamics by FACT promotes transcriptional restart after UV-induced DNA damage.
Marteijn et al., Rotterdam, Netherlands. In Mol Cell, 2013
This accelerated exchange of H2A/H2B is facilitated by SPT16, one of the two subunits of the histone chaperone FACT (facilitates chromatin transcription) but largely independent of its partner SSRP1.
Complex mutual regulation of facilitates chromatin transcription (FACT) subunits on both mRNA and protein levels in human cells.
Gurova et al., Buffalo, United States. In Cell Cycle, 2013
Facilitates chromatin transcription (FACT) is a chromatin remodeling complex with two subunits: SSRP1 and SPT16.
Rpd3- and spt16-mediated nucleosome assembly and transcriptional regulation on yeast ribosomal DNA genes.
Smith et al., Charlottesville, United States. In Mol Cell Biol, 2013
The Spt16 subunit of FACT, however, was specifically required for H2B deposition, suggesting specificity for the H2A/H2B dimer.
Myogenin recruits the histone chaperone facilitates chromatin transcription (FACT) to promote nucleosome disassembly at muscle-specific genes.
Davie et al., Carbondale, United States. In J Biol Chem, 2013
The FACT complex is composed of two subunits: SSRP1 and SPT16.
Histone methyltransferase SETD2 coordinates FACT recruitment with nucleosome dynamics during transcription.
de Almeida et al., Lisbon, Portugal. In Nucleic Acids Res, 2013
Reduction of SETD2 prevents normal loading of the FACT (FAcilitates Chromatin Transcription) complex subunits SPT16 and SSRP1, and decreases nucleosome occupancy in active genes.
Identification of differentially expressed genes of Trichinella spiralis larvae after exposure to host intestine milieu.
Wang et al., Zhengzhou, China. In Plos One, 2012
The results of real-time PCR showed that the expression of nine genes (Ts7, Ndr family protein; Ts8, serine/threonine-protein kinase polo; Ts11, proteasome subunit beta type-7; Ts17, nudix hydrolase; Ts19, ovochymase-1; Ts22, fibronectin type III domain protein; Ts23, muscle cell intermediate filament protein OV71; Ts26, neutral and basic amino acid transport protein rBAT and Ts33, FACT complex subunit SPT16) from 33 T. spiralis genes in IIL were up-regulated compared with that of ML.
The histone H3 Lys 27 demethylase JMJD3 regulates gene expression by impacting transcriptional elongation.
Shi et al., Boston, United States. In Genes Dev, 2012
Furthermore, JMJD3 and KIAA1718 also play a role in localizing elongation factors SPT6 and SPT16 to the target genes.
Identification of Mutant Versions of the Spt16 Histone Chaperone That Are Defective for Transcription-Coupled Nucleosome Occupancy in Saccharomyces cerevisiae.
Martens et al., Pittsburgh, United States. In G3 (bethesda), 2012
We have previously shown, consistent with this function, that Spt16 facilitates repression of the Saccharomyces cerevisiae SER3 gene by maintaining nucleosome occupancy over the promoter of this gene as a consequence of intergenic transcription of SRG1 noncoding DNA.
Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2.
Culotta et al., Baltimore, United States. In Biochem Biophys Res Commun, 2012
Two classes of suppressors were isolated, including the histone chaperones SPT16 and HPC2, and RAD23, a well-conserved protein involved in DNA repair and proteosomal degradation.
Experimentally controlled downregulation of the histone chaperone FACT in Plasmodium berghei reveals that it is critical to male gamete fertility.
Waters et al., Leiden, Netherlands. In Cell Microbiol, 2011
Human FACT (facilitates chromatin transcription) consists of the proteins SPT16 and SSRP1 and acts as a histone chaperone in the (dis)assembly of nucleosome (and thereby chromatin) structure during transcription and DNA replication.
The H2B ubiquitin ligase RNF40 cooperates with SUPT16H to induce dynamic changes in chromatin structure during DNA double-strand break repair.
Johnsen et al., Göttingen, Germany. In Cell Cycle, 2011
SUPT16H and RNF40 are required for proper DNA end resection and timely DNA repair after double-strand breaks.
Expression of FACT in mammalian tissues suggests its role in maintaining of undifferentiated state of cells.
Gurova et al., Buffalo, United States. In Oncotarget, 2011
The Facilitates Chromatin Transcription (FACT) chromatin remodeling complex, comprised of two subunits, SSRP1 and SPT16, is involved in transcription, replication and DNA repair.
Insight into the mechanism of nucleosome reorganization from histone mutants that suppress defects in the FACT histone chaperone.
Formosa et al., Salt Lake City, United States. In Genetics, 2011
Mutation that encodes the Spt16 subunit of FACT causes phenotypes associated with defects in transcription and replication.
Histone variant H2A.Z and RNA polymerase II transcription elongation.
Smith et al., Charlottesville, United States. In Mol Cell Biol, 2011
Here we show that dominant mutations in the elongation genes SPT5 and SPT16 suppress the hypersensitivity of htz1Δ strains to drugs that inhibit elongation, indicating that Htz1 functions at the level of transcription elongation.
Ubiquitylation of FACT by the cullin-E3 ligase Rtt101 connects FACT to DNA replication.
Zhang et al., Rochester, United States. In Genes Dev, 2010
These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication.
New mutant versions of yeast FACT subunit Spt16 affect cell integrity.
Singer et al., Halifax, Canada. In Mol Genet Genomics, 2009
The FACT subunit Spt16 and Pkc1 signaling have an overlapping essential function, with an unexpected role for FACT in the maintenance of cell integrity.
Histone chaperone spt16 promotes redeposition of the original h3-h4 histones evicted by elongating RNA polymerase.
Strubin et al., Genève, Switzerland. In Mol Cell, 2009
Spt16 restores normal nucleosome structure by redepositing the displaced H3-H4 histones, thereby preventing incorporation of new histones.
The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins.
Reinberg et al., United States. In Nature, 1999
Here we show that FACT comprises a new human homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein and the high-mobility group-1-like protein structure-specific recognition protein-1.
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