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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Dec 2016.

Ribonuclease P/MRP 30kDa subunit

Top mentioned proteins: Rpp29, Rpp38, CAN, Ribonuclease P, HAD
Papers on Rpp30
Functional implication of archaeal homologues of human RNase P protein pair Pop5 and Rpp30.
Kimura et al., Fukuoka, Japan. In J Biochem, Jan 2016
PhoPop5 and PhoRpp30 in the hyperthermophilic archaeon Pyrococcus horikoshii, homologues of human ribonuclease P (RNase P) proteins hPop5 and Rpp30, respectively, fold into a heterotetramer [PhoRpp30-(PhoPop5)2-PhoRpp30], which plays a crucial role in the activation of RNase P RNA (PhopRNA).
tRNA processing defects induce replication stress and Chk2-dependent disruption of piRNA transcription.
Huynh et al., Paris, France. In Embo J, Jan 2016
We have isolated mutations in Rpp30, a subunit of RNase P, and find that these induce complete sterility in Drosophila females.
A novel genetic- and cell-based tool for assessing the efficacy and toxicity of anticancer drugs in vitro.
Hanken et al., Hamburg, Germany. In Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, Dec 2015
After the treatment, DNA was prepared directly from the survived adhesive cells in the wells of the 96-well plates using a simple and inexpensive method, and subjected to digital PCR for measuring relative copy numbers of a target gene NF1 to that of a reference gene RPP30.
Determination of True ERBB2 Gene Amplification in Breast Cancer by Quantitative PCR Using a Reference and a Novel Control Gene.
Madoz-Gúrpide et al., Madrid, Spain. In Appl Immunohistochem Mol Morphol, Apr 2015
The QPA is a computation of the relative number of copies of the ERBB2 gene with respect to a nonstandard, short-arm centromeric sequence on chromosome 17, and referenced to a single-copy gene, RPP30.
Newborn blood spot screening test using multiplexed real-time PCR to simultaneously screen for spinal muscular atrophy and severe combined immunodeficiency.
Vogt et al., Atlanta, United States. In Clin Chem, Feb 2015
DBS punches were placed in 96-well arrays, washed, and amplified directly using reagents specific for TREC, a reference gene [ribonuclease P/MRP 30kDa subunit (RPP30)], and the SMN1 gene.
The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA.
Gopalan et al., Columbus, United States. In Nucleic Acids Res, 2015
Indeed, footprinting experiments with an enzyme assembled with the Pfu RPR and five protein cofactors (POP5, RPP21, RPP29, RPP30 and L7Ae-EDTA-Fe) revealed specific RNA cleavages, localizing the binding sites of L7Ae to the RPR's catalytic and specificity domains.
On archaeal homologs of the human RNase P proteins Pop5 and Rpp30 in the hyperthermophilic archaeon Thermococcus kodakarensis.
Kimura et al., Fukuoka, Japan. In Biosci Biotechnol Biochem, 2014
The ribonuclease P (RNase P) proteins TkoPop5 and TkoRpp30, homologs of human Pop5 and Rpp30, respectively, in the hyperthermophilic archaeon Thermococcus kodakarensis were prepared and characterized with respect to pre-tRNA cleavage activity using the reconstitution system of the well-studied Pyrococcus horikoshii RNase P. The reconstituted particle containing TkoPop5 in place of the P. horikoshii counterpart PhoPop5 retained pre-tRNA cleavage activity comparable to that of the reconstituted P. horikoshii RNase P, while that containing TkoRpp30 instead of its corresponding protein PhoRpp30 had slightly lower activity than the P. horikoshii RNase P.
Comparative Analysis of Cell-Associated HIV DNA Levels in Cerebrospinal Fluid and Peripheral Blood by Droplet Digital PCR.
Ellis et al., San Diego, United States. In Plos One, 2014
HIV DNA and the host housekeeping gene (RPP30) were measured in CSF and PBMC by (dd)PCR.
Quantification of cell-free DNA in normal and complicated pregnancies: overcoming biological and technical issues.
Robinson et al., Vancouver, Canada. In Plos One, 2013
Two fetal- or placental-specific duplex assays (RPP30/SRY and RASSF1A/β-Actin) were applied using two technologies, real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR).
Pathway analysis of genome-wide association study for bone mineral density.
Song et al., Seoul, South Korea. In Mol Biol Rep, 2012
Further examination of the gene contents revealed that DBR1, DICER1, EXO1, FEN1, POP1, POP4, RPP30, and RPP38 were involved in 2 of the 8 pathways (p < 0.05).
Thermodynamics of coupled folding in the interaction of archaeal RNase P proteins RPP21 and RPP29.
Foster et al., Columbus, United States. In Biochemistry, 2012
In archaea, RNase P has been shown to be composed of one catalytic RNA and up to five proteins, four of which associate in the absence of RNA as two functional heterodimers, POP5-RPP30 and RPP21-RPP29.
GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential for female gametogenesis and male competence in Arabidopsis.
Yang et al., Beijing, China. In Plos One, 2011
GAF1 is featured as a typical RPP30 domain protein and interacts physically with AtPOP5, a homologue of RNases P/MRP subunit POP5 of yeast.
Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes.
Brusco et al., Torino, Italy. In Biol Proced Online, 2010
During a recent work, where a knockin SCA28 mouse was prepared, we developed two assays based on Real-Time PCR using both SYBR Green and specific minor groove binder (MGB) probes to evaluate the copies of NEO using the comparative delta-delta Ct method versus the Rpp30 reference gene.We compared the results from Southern blot, routinely used to quantify NEO copies, with the two Real-Time PCR assays.
Assembly of the complex between archaeal RNase P proteins RPP30 and Pop5.
Foster et al., Columbus, United States. In Archaea, 2010
Archaeal RNase P consists of one RNA and up to five proteins (Pop5, RPP30, RPP21, RPP29, and RPP38/L7Ae).
Ribosomal protein L7Ae is a subunit of archaeal RNase P.
Gopalan et al., Columbus, United States. In Proc Natl Acad Sci U S A, 2010
After establishing in vitro reconstitution of the single RNA with four previously known protein subunits (POP5, RPP21, RPP29, and RPP30), we show that addition of L7Ae to this RNase P complex increases the optimal reaction temperature and k(cat)/K(m) (by approximately 360-fold) for pre-tRNA cleavage to those observed with partially purified native Mma RNase P. We identify in the Mma RNase P RNA a putative kink-turn (K-turn), the structural motif recognized by L7Ae.
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