Rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acids.
New Haven, United States. In Nucleic Acids Res, Jan 2016
This improved tRNA was tested as substrate for wild-type PylRS as well as three characterized PylRS variants (N(ϵ)-acetyllysyl-tRNA synthetase [AcKRS], 3-iodo-phenylalanyl-tRNA synthetase [IFRS], a broad specific PylRS variant [PylRS-AA]) to incorporate ncAAs at UAG codons in super-folder green fluorescence protein (sfGFP).
Aminoacyl-tRNA synthetase inhibitors as potent antibacterials.
Nanjing, China. In Curr Med Chem, 2011
In this review, we examine the latest developments and structure-activity relationship (SAR) analysis of aminoacyl-tRNA synthetases inhibitors, including methionyl-tRNA synthetase, isoleucyl-tRNA synthetase and phenylalanyl-tRNA synthetase inhibitors.
Göttingen, Germany. In Biol Chem Hoppe Seyler, 1996
The alpha 2 beta 2 enzymes exhibit similarities to PheRS (also an alpha 2 beta 2 enzyme).