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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Dec 2016.


This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011] (from NCBI)
Top mentioned proteins: focal adhesion kinase, Actin, V1a, Src, CAN
Papers using paxillin antibodies
The ‘ins’ and ‘outs’ of podosomes and invadopodia: characteristics, formation and function.
Stradal Theresia E. B., In PLoS ONE, 2010
... against green fluorescent protein (GFP) were purchased from Clontech (Heidelberg, Germany); against zyxin, gelsolin, and paxillin from BD Biosciences (Heidelberg, Germany), against vinculin ...
Quantification of PtdInsP3 molecular species in cells and tissues by mass spectrometry.
Parsons Maddy, In PLoS ONE, 2010
... Anti-paxillin antibody (clone 5H11) was from Millipore; Anti-PIPKIγ polyclonal antibody was from Cell Signaling Technology; Anti-tubulin antibody and ...
A role for the juxtamembrane cytoplasm in the molecular dynamics of focal adhesions.
Parsons Maddy, In PLoS ONE, 2008
... For detection of both paxillin and Hic-5 by immunofluorescence mouse anti-paxillin (clone 349) was used (BD Biosciences).
Elucidation of CXCR7-mediated signaling events and inhibition of CXCR4-mediated tumor cell transendothelial migration by CXCR7 ligands.
Cookson Mark R., In PLoS ONE, 2008
... Antibodies for monitoring focal adhesion proteins were: total FAK, phospho-FAK (Y397), total paxillin, total p130Cas (Cell Signaling), and phospho-paxillin (Y118), phospho-p130Cas ...
Thrombospondin-1 enhances human thyroid carcinoma cell invasion through urokinase activity.
Hooker Bill, In PLoS ONE, 2007
... Anti-paxillin (clone 349) antibody was from BD Biosciences (Le Pont de Claix, ...
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Papers on paxillin
Substrate Stiffness Modulates Lung Cancer Cell Migration but not Epithelial to Mesenchymal Transition.
Ghadiali et al., Columbus, United States. In J Biomed Mater Res A, Feb 2016
We also observed decreased levels of phosphorylated focal adhesion kinase (FAK) and paxillin on stiffer substrates which correlated with slower cell migration.
Hic-5 mediates endothelial sprout initiation by regulating a key surface metalloproteinase.
Bayless et al., College Station, United States. In J Cell Sci, Feb 2016
Pro-angiogenic factors enhanced co-localization and complex formation between membrane type-1 matrix metalloproteinase (MT1-MMP) and Hic-5, but not paxillin, and the LIM2 and LIM3 domains of Hic-5 were necessary and sufficient for MT1-MMP complex formation.
Epithelial- mesenchymal transition, IP3 receptors and ER-PM junctions: translocation of Ca2+ signalling complexes and regulation of migration.
Tepikin et al., Liverpool, United Kingdom. In Biochem J, Feb 2016
Uncaging of IP3 resulted in prominent accumulation of paxillin in focal adhesions, highlighting important functional implications of the observed novel structural relationships.
Activation of dickkopf-1 and focal adhesion kinase pathway by tumour necrosis factor α induces enhanced migration of fibroblast-like synoviocytes in rheumatoid arthritis.
Kim et al., Taegu, South Korea. In Rheumatology (oxford), Jan 2016
Moreover, migrating FLSs exhibited significantly higher levels of FAK, p-JNK, paxillin and cdc42 expression, whereas the level of cytosolic β-catenin was lower.
Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly.
Humphries et al., Manchester, United Kingdom. In Nat Cell Biol, Dec 2015
The consensus adhesome is likely to represent a core cell adhesion machinery, centred around four axes comprising ILK-PINCH-kindlin, FAK-paxillin, talin-vinculin and α-actinin-zyxin-VASP, and includes underappreciated IAC components such as Rsu-1 and caldesmon.
Mechano-regulatory cellular behaviors of NIH/3T3 in response to the storage modulus of liquid crystalline substrates.
Tu et al., Taiwan. In J Mech Behav Biomed Mater, Dec 2015
Our results demonstrated that NIH/3T3 cells showed a hypersensitive response to the storage modulus of liquid crystalline substrates by the alteration in attachment, spreading, proliferation and viability, polarization, cell cycle and apoptosis, and activity of mechano-transduction-related signal molecules including FAK, paxillin and ERK.
Non-receptor tyrosine kinases and the actin cytoskeleton in contractile vascular smooth muscle.
Ohanian et al., Manchester, United Kingdom. In J Physiol, Oct 2015
Recent work has shown that non-receptor tyrosine kinases and tyrosine phosphorylation of focal adhesion proteins such as paxillin and Hic-5 are important for actin cytoskeleton and focal adhesion remodelling and contraction.
Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions.
Waterman et al., Bethesda, United States. In Nat Cell Biol, Jul 2015
Inactive vinculin localizes to the lower integrin signalling layer in FAs by binding to phospho-paxillin.
A novel role for RhoA GTPase in the regulation of airway smooth muscle contraction.
Gunst et al., Indianapolis, United States. In Can J Physiol Pharmacol, Feb 2015
Contractile stimulation of ASM induces the recruitment and assembly of paxillin, vinculin, and focal adhesion kinase (FAK) into membrane adhesion complexes (adhesomes) that regulate actin polymerization by catalyzing the activation of cdc42 GTPase by the G-protein-coupled receptor kinase-interacting target (GIT) - p21-activated kinase (PAK) - PAK-interacting exchange factor (PIX) complex.
Tyrosyl phosphorylated serine-threonine kinase PAK1 is a novel regulator of prolactin-dependent breast cancer cell motility and invasion.
Diakonova et al., Toledo, United States. In Adv Exp Med Biol, 2014
Tyrosyl phosphorylated PAK1 facilitates PRL-dependent motility via at least two mechanisms: formation of paxillin/GIT1/βPIX/pTyr-PAK1 complexes resulting in increased adhesion turnover and phosphorylation of actin-binding protein filamin A. Increased adhesion turnover is the basis for cell migration and phosphorylated filamin A stimulates the kinase activity of PAK1 and increases actin-regulating activity to facilitate cell motility.
Multi-targeted molecular therapeutic approach in aggressive neuroblastoma: the effect of Focal Adhesion Kinase-Src-Paxillin system.
Theocharis et al., Philadelphia, United States. In Expert Opin Ther Targets, 2014
The focal adhesion kinase (FAK)-Src-paxillin complex, a major mediator of the integrin pathway, contributes to cell migration and motility.
Intracellular signaling and perception of neuronal scaffold through integrins and their adapter proteins.
Bastmeyer et al., Genève, Switzerland. In Prog Brain Res, 2013
While we address some newer findings concerning the recruitment of paxillin to the integrin-talin-kindlin complex, we will also highlight the central function of paxillin in integrin signaling and how it can interface with other signaling systems controlling neuronal or synaptic functions.
Structural basis for hijacking of cellular LxxLL motifs by papillomavirus E6 oncoproteins.
Travé et al., Illkirch-Graffenstaden, France. In Science, 2013
We solved the crystal structures of bovine (BPV1) and human (HPV16) papillomavirus E6 proteins bound to LxxLL peptides from the focal adhesion protein paxillin and the ubiquitin ligase E6AP, respectively.
Cell adhesion-dependent serine 85 phosphorylation of paxillin modulates focal adhesion formation and haptotactic migration via association with the C-terminal tail domain of talin.
Lee et al., Seoul, South Korea. In J Biol Chem, 2012
cell adhesion-dependent Ser-85 phosphorylation of paxillin is important for its interaction with talin and regulation of dynamic FAs and cell migration.
Paxillin phosphorylation by JNK and p38 is required for NFAT activation.
Lai et al., Taipei, Taiwan. In Eur J Immunol, 2012
results demonstrate that dual phosphorylation of paxillin by JNK and p38 MAPK is essential for T-cell activation and suggest that NFAT is a functional target of the JNK/p38 phosphorylated paxillin
Identification of paxillin domains interacting with β-catenin.
Birukov et al., Chicago, United States. In Febs Lett, 2012
analysis of paxillin domains interacting with beta-catenin
Paxillin mediates extranuclear and intranuclear signaling in prostate cancer proliferation.
Hammes et al., Rochester, United States. In J Clin Invest, 2012
paxillin is a liaison between extranuclear MAPK signaling and nuclear transcription in response to androgens and growth factors
Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.
Lewis et al., Cambridge, United Kingdom. In Gene Expr Patterns, 2012
Although Pxn expression is visible throughout the cochlear duct, at pre- and early post-natal stages it is always most marked in the pillar cells.
Nanoscale architecture of integrin-based cell adhesions.
Waterman et al., Bethesda, United States. In Nature, 2010
Our results reveal that integrins and actin are vertically separated by a ∼40-nm focal adhesion core region consisting of multiple protein-specific strata: a membrane-apposed integrin signalling layer containing integrin cytoplasmic tails, focal adhesion kinase and paxillin; an intermediate force-transduction layer containing talin and vinculin; and an uppermost actin-regulatory layer containing zyxin, vasodilator-stimulated phosphoprotein and α-actinin.
A distinctive role for focal adhesion proteins in three-dimensional cell motility.
Wirtz et al., Baltimore, United States. In Nat Cell Biol, 2010
Live-cell microscopy shows that for cells fully embedded in a 3D matrix, focal adhesion proteins, including vinculin, paxillin, talin, alpha-actinin, zyxin, VASP, FAK and p130Cas, do not form aggregates but are diffusely distributed throughout the cytoplasm.
More papers using paxillin antibodies
Heparanase is involved in angiogenesis in esophageal cancer through induction of cyclooxygenase-2.
Orgel Joseph P. R. O., In PLoS ONE, 2004
... Antibodies to syndecan-4 and paxillin were purchased from Santa Cruz Biotechnology (Sanata Cruz, CA) ...
In situ measurement of degranulation as a biosensor based on RBL-2H3 mast cells.
Zhang Xin, In PLoS ONE, 2003
... Mouse monoclonal antibody to Csk and Paxillin were from BD Biosciences (San Jose, CA, USA) ...
Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
Beckerle Mary C. et al., In The Journal of Cell Biology, 2003
... Commercial antibody sources included ICN Biomedicals (α-actinin), Sigma-Aldrich (talin and vinculin), BD Biosciences (caldesmon, ILK, paxillin, and integrins α3, αV, β1, β3), Upstate Cell Signaling (FAK 4–47, pY-4G10, and ...
PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility
Schlaepfer David D. et al., In The Journal of Cell Biology, 2002
... mAbs to p190RhoGAP (clone 30), Pyk2 (clone 11), paxillin (clone M107), and phosphospecific paxillin pTyr-118 (clone 30) were obtained from BD Biosciences.
Beta-arrestin- and c-Src-dependent degradation of G-protein-coupled receptor kinase 2.
Buday Laszlo, In PLoS ONE, 2000
... Antibodies against paxillin were purchased from Abcam.
The relationship between force and focal complex development
Sheetz Michael P. et al., In The Journal of Cell Biology, 1999
... Anti–human vinculin antibody was obtained from Sigma-Aldrich, and ligand-occupied β1 (9EG7) and anti–paxillin antibodies were obtained from BD Biosciences.
Phosphorylation of actopaxin regulates cell spreading and migration
Turner Christopher E. et al., In The Journal of Cell Biology, 1999
... Primary antibodies to the Xpress epitope were purchased from Invitrogen and paxillin (clone 165) were purchased from BD Biosciences.
Rap1 promotes cell spreading by localizing Rac guanine nucleotide exchange factors
Cooper Jonathan A. et al., In The Journal of Cell Biology, 1998
... The following antibodies were obtained: rabbit anti-Rap1 (Santa Cruz Biotechnology, Inc.), mouse anti-Rac1 and -Paxillin (BD Biosciences), VAV2 rabbit anti-sera (provided ...
Quantifying Lamella Dynamics of Cultured Cells by SACED, a New Computer-Assisted Motion Analysis.
Cimini Daniela, In PLoS ONE, 1998
... Monoclonal antibodies (mAb) to Rac1, beta-catenin and paxillin were purchased from Transduction Laboratories (Becton Dickinson, Amsterdam, The Netherlands) ...
Cell adaptive response to extracellular matrix density is controlled by ICAP-1–dependent β1-integrin affinity
Albiges-Rizo Corinne et al., In The Journal of Cell Biology, 1998
... Paxillin mAb (349) was purchased from BD Biosciences.
Camp-sensitive and rapamycin-sensitive regulation of the association of eukaryotic initiation-factor 4e and the translational regulator Phas-I in aortic smooth-muscle cells
Bond Mark et al., In Journal of Molecular and Cellular Cardiology, 1994
... hyperphosphorylated-Rb, phospho-ERK, ERK, phospho-JNK, JNK (#9308, #9101, #9102, #9251, #9258; Cell Signalling), V5-tag (#R960-25; Invitrogen), paxillin (#610051; BD Biosciences), BrdU (#B2531; Sigma) and ...
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