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nebulin, Neb
This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein. The encoded protein contains approximately 30-amino acid long modules that can be classified into 7 types and other repeated modules. Protein isoform sizes vary from 600 to 800 kD due to alternative splicing that is tissue-, species-,and developmental stage-specific. Of the 183 exons in the nebulin gene, at least 43 are alternatively spliced, although exons 143 and 144 are not found in the same transcript. Of the several thousand transcript variants predicted for nebulin, the RefSeq Project has decided to create three representative RefSeq records. Mutations in this gene are associated with recessive nemaline myopathy. [provided by RefSeq, Sep 2009] (from NCBI)
Top mentioned proteins: Actin, titin, CAN, HAD, ACID
Papers using nebulin antibodies
Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors
Leong Kam W et al., In Molecular Therapy. Nucleic Acids, 2010
... coding sequence from pmax-GFP with SacI digestion, blunting by DNA polymerase I Klenow fragment, NheI-HF digestion (NEB, Ipswich, MA), and gel extraction (QIAquick Gel Extraction Kit; QIAGEN, Hilden, Germany) ...
Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli
Zhu Jess et al., In Journal of Biomedicine and Biotechnology, 2010
... -NTA resin, and mouse anti-human CysC monoclonal antibodies were obtained from NEB (Beijing LTD., Beijing, China), QIAGEN (Dusseldorf, Germany), and Sigma ...
Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules
Fortier Michel A. et al., In Frontiers in Pharmacology, 2009
... TAQ DNA polymerase and buffer used for polymerase chain reaction were from NEB (New England Biolabs, ON, Canada) ...
SLC45A3-ELK4 is a novel and frequent erythroblast transformation-specific fusion transcript in prostate cancer.
Preiss Thomas, In PLoS ONE, 2008
... For poly(A) tailing, 50 ng RNA was incubated with 5U poly(A) polymerase (NEB) and 1 mM ATP, then cleaned on QIAQuick columns (QIAgen).
Silencing Mycobacterium smegmatis by using tetracycline repressors.
Doherty T. Mark, In PLoS ONE, 2006
... NEB-10β (New England Biolabs UK Ltd) were used ...
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Papers on nebulin
Response to dietary-induced energy restriction in dairy sheep divergently selected for resistance or susceptibility to mastitis.
Rupp et al., France. In J Dairy Sci, Jan 2016
Dairy ruminants experiencing a severe postpartum negative energy balance (NEB) are considered to be more susceptible to mastitis.
Understanding the carbon-monoxide oxidation mechanism on ultrathin palladium nanowires: a density functional theory study.
Hsieh et al., Kao-hsiung, Taiwan. In Nanoscale, Jan 2016
The nudged elastic band (NEB) method was adopted to obtain the transition state configuration and the minimum energy pathways (MEPs).
Theoretical study of carbon dioxide activation by metals (Co, Cu, Ni) supported on activated carbon.
Cam et al., Vietnam. In J Mol Model, Dec 2015
The activation of carbon dioxide (CO2) by catalytic systems comprising a transition metal (Co, Cu,Ni) on an activated carbon (AC) support was investigated using a combination of different theoretical calculation methods: Monte Carlo simulation, DFT and DFT-D, molecular dynamics (MD), and a climbing image nudged elastic band (CI-NEB) method.
Targeted next-generation sequencing assay for detection of mutations in primary myopathies.
Hackman et al., Helsinki, Finland. In Neuromuscul Disord, Dec 2015
The large number of possible candidate genes and overlapping phenotypes, as well as an enormous size of some of the genes such as DMD, TTN and NEB, constitute difficult challenges for molecular genetic diagnostics using conventional sequencing.
Hyperplasia of pulmonary neuroendocrine cells in infancy and childhood.
Cutz, Toronto, Canada. In Semin Diagn Pathol, Nov 2015
Pulmonary neuroendocrine cells (PNEC) are widely distributed throughout the airway mucosa of mammalian lung as solitary cells and as distinctive innervated clusters, neuroepithelial bodies (NEB).
Targeting the Atypical Chemokine Receptor ACKR3/CXCR7: Phase 1 - Phage Display Peptide Identification and Characterization.
Taylor et al., Greensboro, United States. In Curr Top Med Chem, Oct 2015
A 20 amino acid region of the ACKR3/CXCR7 N-terminus was synthesized and targeted with the NEB PhD™-7 Phage Display Peptide Library.
Molecular insights into the mechanisms of liver-associated diseases in early-lactating dairy cows: hypothetical role of endoplasmic reticulum stress.
Eder et al., Gießen, Germany. In J Anim Physiol Anim Nutr (berl), Aug 2015
Despite the fact that all high-yielding dairy cows are affected by marked metabolic stress due to a severe negative energy balance (NEB) during early lactation, not all cows develop liver-associated diseases.
An update on the LIM and SH3 domain protein 1 (LASP1): a versatile structural, signaling, and biomarker protein.
Grunewald et al., Würzburg, Germany. In Oncotarget, 2015
As the first protein of a class comprising one N-terminal LIM and one C-terminal SH3 domain, LASP1 founded a new LIM-protein subfamily of the nebulin group.
β-Hydroxybutyrate Facilitates Fatty Acids Synthesis Mediated by Sterol Regulatory Element-Binding Protein1 in Bovine Mammary Epithelial Cells.
Li et al., In Cell Physiol Biochem, 2014
Ketotic cows are characterized by excessive negative energy balance (NEB), which can further increase the blood BHBA concentration.
Roles of Nebulin Family Members in the Heart.
Chen et al., Milano, Italy. In Circ J, 2014
The members of the nebulin protein family, including nebulin, nebulette, LASP-1, LASP-2, and N-RAP, contain various numbers of nebulin repeats and bind to actin, but are otherwise heterogeneous with regard to size, expression pattern, and function.
A two-segment model for thin filament architecture in skeletal muscle.
Fowler et al., Los Angeles, United States. In Nat Rev Mol Cell Biol, 2013
This is in contrast to the classic 'nebulin ruler' model, which postulates that thin filaments are uniform structures, the lengths of which are dictated by nebulin.
Carrier state for the nebulin exon 55 deletion and abnormal prenatal ultrasound findings as potential signs of nemaline myopathy.
Pras et al., Ramat Gan, Israel. In Prenat Diagn, 2012
Ultrasonographic findings suggestive of a myopathy and a carrier state for the NEB exon 55 deletion in one of the parents should trigger a thorough investigation for nemaline.
Nemaline myopathy caused by mutations in the nebulin gene may present as a distal myopathy.
Wallgren-Pettersson et al., Helsinki, Finland. In Neuromuscul Disord, 2011
distal nemaline myopathy caused by four different compound heterozygous nebulin mutations
Disrupted myosin cross-bridge cycling kinetics triggers muscle weakness in nebulin-related myopathy.
Larsson et al., Uppsala, Sweden. In Faseb J, 2011
The mechanics as well as the X-ray diffraction patterns of human membrane-permeabilized single muscle fibers expressing nebulin mutations are reported.
Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation.
Endo et al., Chiba, Japan. In Science, 2011
findings show the nebulin-N-WASP complex caused actin nucleation for unbranched actin filament formation from the Z bands without the Arp2/3 complex
Nebulin regulates actin filament lengths by a stabilization mechanism.
Gregorio et al., Tucson, United States. In J Cell Biol, 2010
Nebulin regulates thin filament architecture by a mechanism that includes stabilizing the filaments and preventing actin depolymerization.
Muscle giants: molecular scaffolds in sarcomerogenesis.
Bloch et al., Baltimore, United States. In Physiol Rev, 2009
Three giant, muscle-specific proteins, titin (3-4 MDa), nebulin (600-800 kDa), and obscurin (approximately 720-900 kDa), have been proposed to play important roles in the assembly and stabilization of sarcomeres.
Molecular diversity of myofibrillar proteins: gene regulation and functional significance.
Reggiani et al., Padova, Italy. In Physiol Rev, 1996
Additional isoforms, including products of tropomyosin, myosin light chain 1 fast, troponin T, titin, and nebulin genes, can be generated from the same gene through alternative splicing or use of alternative promoters.
A physiological role for titin and nebulin in skeletal muscle.
Podolsky et al., In Nature, 1986
Recently, the two exceptionally large proteins titin (also called connectin) and nebulin (originally called band 3) have been implicated in passive elasticity (for review see refs 7, 8).
More papers using nebulin antibodies
A novel enzyme-linked immunosorbent assay for diagnosis of Mycobacterium avium subsp. paratuberculosis infections (Johne’s disease) in cattle
Mundo Silvia Leonor et al., In Veterinary Medicine International, 2005
... -specific immunomagnetic beads (NEB, New England Biolabs, Ipswich, MA, USA) [ ...
DNA motifs associated with aberrant CpG island methylation
van Steensel Bas, In PLoS Genetics, 2005
... μl containing 20 U of SssI methylase, 320 μM S-adenosylmethionine (SAM, New England Biolabs), and 1× NEB buffer 2 (New England Biolabs).
Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family
Terwilliger Thomas C. et al., In Journal of Structural and Functional Genomics, 2004
... The PCR amplicon was digested with NdeI and BamHI restriction endonucleases (NEB), and cleaned using Qiaquick PCR spin column (Qiagen).
Hsp70-2 is required for tumor cell growth and survival
O'Rand Michael G et al., In Reproductive Biology and Endocrinology : RB&E, 2004
... Restriction enzymes and DNA ligase were obtained from NEB (New England Biolabs, Ipswich, MA) ...
Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction
Perler Francine B. et al., In Biochemistry, 2003
... cells (NEB TURBO and T7 Express) were obtained from New England Biolabs (NEB) ...
Insights into the conformational equilibria of maltose-binding protein by analysis of high affinity mutants
Riggs Paul D. et al., In Applied Microbiology and Biotechnology, 2002
... Host strains TB1, ER1992, ER2502, ER2984, NEB 5-alpha, NEB Turbo, and NEB Express were obtained from New England Biolabs.
Using sequence logos and information analysis of Lrp DNA binding sites to investigate discrepancies between natural selection and SELEX.
Guttman David S., In PLoS Genetics, 1998
... The DNA was made double stranded by second strand synthesis with Klenow (NEB), and the fragments were purified with a QIAquick PCR purification kit (Qiagen).
Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses
Rossi John J. et al., In Nucleic Acids Research, 1996
... all chemicals were purchased from Sigma-Aldrich, all restriction enzymes were obtained from New England BioLabs (NEB) and all cell culture products were purchased from GIBOC (Gibco BRL/Life Technologies, a division of Invitrogen) ...
Maximum likelihood phylogenetic estimation from DNA sequences with variable rates over sites: approximate methods.
Kolokotronis Sergios-Orestis, In PLoS ONE, 1993
... 5 μg of chloroplast DNA was fragmented using dsDNA Fragmentase (NEB, USA) at 37 °C for 30 min, then fragmented DNA was purified using MinElute column (Qiagen, Germany) and eluted in ...
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