Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes.
Bielefeld, Germany. In Hum Reprod, Jan 2016
Denuded in vivo or in vitro matured MII oocytes were postovulatory aged and analyzed by semiquantitative confocal microscopy for abundance and localization of polyadenylated RNAs, proteins of maternal effect genes (transcription activator BRG1 also known as ATP-dependent helicase SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4) and NOD-like receptor family pyrin domain containing 5 (NLRP5) also known as MATER), RNA-binding proteins (MSY2 also known as germ cell-specific Y-box-binding protein, YBX2), and post-transcriptionally modified histones (trimethylated histone H3K9 and acetylated histone H4K12), as well as pericentromeric ATRX (alpha thalassemia/mental retardation syndrome X-linked, also termed ATP-dependent helicase ATRX or X-linked nuclear protein (XNP)).
Search for regulatory factors of the pituitary-specific transcription factor PROP1 gene.
Japan. In J Reprod Dev, Jan 2016
Furthermore, a synergy effect with SOX2 was observed for an additional 10 factors, FOXJ1, HES1, HEY1, HEY2, KLF6, MSX1, RUNX1, TEAD2, YBX2 and ZFP36Ll, which did not show substantial independent action.
Mechanisms of translational repression of the Smcp mRNA in round spermatids.
Boston, United States. In Reproduction, 2015
RNA affinity chromatography and mass spectrometry sequencing identified Y-box protein 2 (YBX2/MSY2) as the major protein that interacts with the 3' terminus of the Smcp 3'-UTR and a Y-box recognition sequence, GCCACCU, in the translation control element that is necessary for Prm1 mRNA repression.
Oocyte-specific genes affect folliculogenesis, fertilization, and early development.
Bethesda, United States. In Semin Reprod Med, 2007
Mouse transgenesis has been particularly useful in defining germ-cell specific genes and their roles in folliculogenesis (e.g., DAZLA, FIGLA, NOBOX, SOHLH1, YBX2, CPEB1, GDF9), fertilization (e.g., ZP1, ZP2, ZP3), and preimplantation embryonic development (e.g., NPM2, ZAR1, NALP5, DPPA3).