Expression of epigenetic modifiers is not significantly altered by exposure to secondhand smoke.
Los Angeles, United States. In Lung Cancer, Nov 2015
RESULTS AND CONCLUSION: Quantification of the expression of genes encoding DNA methyltransferases (Dnmt1, Dnmt3a, Dnmt3b and Dnmt3l), methyl binding domain proteins (Mecp2, Mbd2 and Mbd3) and histone deacetylases (Hdac1 and Hdac2) by quantitative reverse-transcription polymerase chain reaction analysis showed modest but not statistically significant differences in the relative transcription of these key epigenetic regulators between SHS-exposed mice and age-matched controls.
Positive and negative regulators of the metallothionein gene (review).
Sagamihara, Japan. In Mol Med Report, Jul 2015
The present review focuses on PU.1 and several other negative regulators of this gene, including PZ120, DNA methyltransferase 3a with Mbd3 and Brg1 complex, CCAAT enhancer binding protein α and Ku protein, and describes the suppression of the MT genes through these transcription factors.
Reprogramming barriers and enhancers: strategies to enhance the efficiency and kinetics of induced pluripotency.
Yazd, Iran. In Cell Regen (lond), 2014
Different strategies have been applied for enhancing reprogramming efficiency, including depletion/inhibition of barriers (p53, p21, p57, p16(Ink4a)/p19(Arf), Mbd3, etc.), overexpression of enhancing genes (e.g., FOXH1, C/EBP alpha, UTF1, and GLIS1), and administration of certain cytokines and small molecules.
MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner.
Cambridge, United Kingdom. In Cell Stem Cell, 2014
By knocking out MBD3, an essential scaffold subunit of the NuRD complex, at different time points in reprogramming, we found that efficient formation of reprogramming intermediates and induced pluripotent stem cells from neural stem cells requires NuRD activity.
MBD2 and MBD3: elusive functions and mechanisms.
Nijmegen, Netherlands. In Front Genet, 2013
This review focuses on two members of the methyl binding proteins, namely MBD2 and MBD3 that reside in very similar complexes, yet appear to have very different biological roles.
Deterministic direct reprogramming of somatic cells to pluripotency.
Israel. In Nature, 2013
Here we show that depleting Mbd3, a core member of the Mbd3/NuRD (nucleosome remodelling and deacetylation) repressor complex, together with OSKM transduction and reprogramming in naive pluripotency promoting conditions, result in deterministic and synchronized iPS cell reprogramming (near 100% efficiency within seven days from mouse and human cells).