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LEU2 Leu2p

LEU2, Lyt-3, 3-Isopropylmalate Dehydrogenase
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010] (from NCBI)
Top mentioned proteins: CAN, ACID, TRPC1, HAD, lacZ
Papers using LEU2 antibodies
Global analysis of protein localization in budding yeast
Lesuisse Emmanuel et al., In Traffic (Copenhagen, Denmark), 2002
... leu2Δmet15Δura5Δhis5ΔARN1-GFP::HIS3Clontech ...
The peroxisome biogenesis disorder group 4 gene, PXAAA1, encodes a cytoplasmic ATPase required for stability of the PTS1 receptor
Gould Stephen J. et al., In The Journal of Cell Biology, 1995
... also cloned into the following vectors: pPC86/LEU2, a dihybrid GAL4 activation domain (AD) fusion vector, creating pPC86/LEU2-PEX19; pMBP, a modified form of the pMALc2 expression vector (New England Biolabs Inc.); and pT7-6xHis, a modified ...
Papers on LEU2
Connective tissue growth factor differentially binds to members of the cystine knot superfamily and potentiates platelet-derived growth factor-B signaling in rabbit corneal fibroblast cells.
Schultz et al., Gainesville, United States. In World J Biol Chem, Dec 2015
EYG48 reporter strain that carried a wild-type LEU2 gene under the control of LexA operators and a lacZ reporter plasmid (p80p-lacZ) containing eight high affinity LexA binding sites were used in the yeast two-hybrid analysis.
Analysis of Meiotic Recombination and Homolog Interaction during Yeast Meiosis.
Cha et al., Bangor, United Kingdom. In Cold Spring Harb Protoc, Oct 2015
Here we present a general protocol for visualizing chromosomal proteins and homolog interaction on surface-spread nuclei and a widely used protocol for analyzing meiotic recombination based on an engineered hotspot referred to as HIS4::LEU2.
Auxotrophic Mutations Reduce Tolerance of Saccharomyces cerevisiae to Very High Levels of Ethanol Stress.
Thevelein et al., Leuven, Belgium. In Eukaryot Cell, Sep 2015
Analysis of other auxotrophies also revealed significant linkage for LYS2, LEU2, HIS3, and MET15.
Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Jin et al., Urbana, United States. In Appl Environ Microbiol, 2014
We successfully constructed a quadruple auxotrophic mutant of a popular industrial polyploid yeast strain, Saccharomyces cerevisiae ATCC 4124, with ura3, trp1, leu2, and his3 auxotrophies through RNA-guided Cas9 nuclease.
Metabolic engineering of Saccharomyces cerevisiae for the production of isobutanol and 3-methyl-1-butanol.
Hahn et al., Seoul, South Korea. In Appl Microbiol Biotechnol, 2014
In this study, we engineered CEN.PK2-1C, a leucine auxotrophic strain having a LEU2 gene mutation, for the production of isobutanol and 3-methyl-1-butanol.
Scarless gene deletion in methylotrophic Hansenula polymorpha by using mazF as counter-selectable marker.
Zhang et al., Beijing, China. In Anal Biochem, 2014
The applicability of the strategies in markerless or scarless deletion of PEP4, LEU2, and TRP1 indicates that this study provides easy, time-efficient, and host-independent protocols for single or multiple genetic manipulations in H. polymorpha.
Dissecting the cis and trans elements that regulate adjacent-gene coregulation in Saccharomyces cerevisiae.
McAlear et al., Middletown, United States. In Eukaryot Cell, 2014
We observed that coupled corepression of the gene pair under heat shock was abrogated when the two genes were separated by an actively expressed RNA polymerase (Pol) II transcription unit (the LEU2 gene) but not when the inserted LEU2 gene was repressed.
Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins.
Kirchhoff et al., Ulm, Germany. In J Virol, 2012
Nef-mediated down-modulation of CD8alphabeta is a fundamental property of primate lentiviruses.
The multiple roles of the CD8 coreceptor in T cell biology: opportunities for the selective modulation of self-reactive cytotoxic T cells.
Sewell et al., Cardiff, United Kingdom. In J Leukoc Biol, 2011
[review] Coreceptor CD8alphabeta contributes to the antigen-recognition process by binding to a largely invariant region of the MHCI molecule and by promoting intracellular signaling.
Novel CD8+ Treg suppress EAE by TGF-beta- and IFN-gamma-dependent mechanisms.
Weiner et al., Boston, United States. In Eur J Immunol, 2009
Data show that CD8+LAP+ cells produce IFN-gamma, and these cells suppress EAE that is dependent on both TGF-beta and IFN-gamma.
Structural basis of the CD8 alpha beta/MHC class I interaction: focused recognition orients CD8 beta to a T cell proximal position.
Margulies et al., Bethesda, United States. In J Immunol, 2009
The crystal structure of CD8alphabeta in complex with H-2Dd antigen indicates that CD8beta is crucial in orienting the CD8alphabeta heterodimer.
A new function for LAT and CD8 during CD8-mediated apoptosis that is independent of TCR signal transduction.
Potter et al., Denver, United States. In Eur J Immunol, 2009
Results identify novel functions for both CD8 and LAT during CD8-mediated apoptosis that are independent of TCR signal transduction and suggest a mechanism for signal transduction leading to apoptosis upon CD8 crosslinking.
Genetic basis of individual differences in the response to small-molecule drugs in yeast.
Kruglyak et al., Cambridge, United States. In Nat Genet, 2007
We also experimentally verified that a deficiency in leucine biosynthesis caused by a deletion of LEU2 underlies sensitivity to niguldipine, which is structurally related to therapeutic calcium channel blockers, and that a natural coding-region polymorphism in the inorganic phosphate transporter PHO84 underlies sensitivity to two polychlorinated phenols that uncouple oxidative phosphorylation.
Leucine biosynthesis in fungi: entering metabolism through the back door.
Kohlhaw, West Lafayette, United States. In Microbiol Mol Biol Rev, 2003
First, the genes and enzymes specific for the leucine pathway are considered: LEU4 and LEU9 (encoding the alpha-isopropylmalate synthase isoenzymes), LEU1 (isopropylmalate isomerase), and LEU2 (beta-isopropylmalate dehydrogenase).
Break-induced replication: a review and an example in budding yeast.
Haber et al., Waltham, United States. In Proc Natl Acad Sci U S A, 2001
Linearized, centromere-containing plasmid DNA with the 5' end of the LEU2 gene at one end was transformed into a strain in which the 5' end of LEU2 was replaced by ADE1, preventing simple homologous gene replacement to become Leu2(+).
Transformation systems of non-Saccharomyces yeasts.
Lee et al., Québec, Canada. In Crit Rev Biotechnol, 2000
The LEU2 gene and G418 resistance gene are the two most popular markers used in the yeast transformation.
Role of an upstream regulatory element in leucine repression of the Saccharomyces cerevisiae leu2 gene.
Casadaban et al., In Nature, 1984
By constructing beta-galactosidase gene fusions, to facilitate measuring gene expression in vivo, we have now defined a cis-acting regulatory element of the Saccharomyces cerevisiae leu2 gene.
Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
Alani et al., In Cell, 1984
Each site, when placed upstream of the transcriptional initiation region of the yeast LEU2 gene, activates LEU2 transcription which is regulated by catabolite repression.
Expression of a bacterial modification methylase gene in yeast.
Venetianer et al., In Nature, 1983
Southern-type hybridization to DNAs isolated from the transformed yeast clones revealed that the yeast plasmid carrying the prokaryotic methylase gene, as well as the two chromosomal genes tested (his3 and leu2) were methylated, whereas the bulk of the yeast DNA remained largely unmethylated.
Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae.
Wu et al., In Nature, 1980
The frequency of unequal crossing over, as measured by the deletion or duplication of an inserted genetic marker (LEU2), is sufficient to maintain the sequence homogeneity of the rDNA repeat units.
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