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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Dec 2016.

LacZ beta-D-galactosidase

lacZ, beta-Galactosidase
Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527]. (from NCBI)
Top mentioned proteins: CAN, V1a, ACID, HAD, OUT
Papers using lacZ antibodies
Macrophages, dendritic cells, and kidney ischemia-reperfusion injury.
Chatziantoniou Christos, In PLoS ONE, 2009
... Mice of this line were bred with Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J transgenic mice expressing rtTA under ...
Complementary cooperation between two syntrophic bacteria in pesticide degradation.
Kao Katy, In PLoS ONE, 2008
... I recognition sites, had been subcloned into the corresponding site of the plasmid, pPROTet.E333-lacZ (Clontech), using the following primers, ...
Diabetes impairs the vascular recruitment of normal stem cells by oxidant damage, reversed by increases in pAMPK, heme oxygenase-1, and adiponectin
Vasquez Elisardo C et al., In Lipids in Health and Disease, 2008
... lacZ gene) transgenic mice (12-week-old) were used ...
Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds
Xie Xin et al., In Cell Research, 2007
... OG2 mice, which carry the Rosa26-lacZ allele and a transgenic Oct4 promoter driving GFP ...
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Papers on lacZ
Spontaneous formation of extensive vessel-like structures in murine engineered heart tissue.
Eschenhagen et al., Hamburg, Germany. In Tissue Eng Part A, Feb 2016
Here we generated fibrin-based mini EHT from a transgenic mouse line (Cdh5-CreERT2 x Rosa26-LacZ) in which EC were specifically and inducible labelled by applying tamoxifen (EC<sup>iLacZ</sup>). EHT were generated from an unpurified cell mix of new-born mouse hearts and were cultured under standard, serum-containing conditions.
Genetic lineage tracing discloses arteriogenesis as the main mechanism for collateral growth in the mouse heart.
Zhou et al., Shanghai, China. In Cardiovasc Res, Feb 2016
METHODS AND RESULTS: Using Apln-GFP and Apln-LacZ reporter mice, we demonstrate that APLN expression is enriched in coronary vascular endothelial cells.
Subretinal AAV2.COMP-Ang1 Suppresses Choroidal Neovascularization and Vascular Endothelial Growth Factor in a Murine Model of Age-Related Macular Degeneration.
Ambati et al., Salt Lake City, United States. In Exp Eye Res, Feb 2016
We treated mice with subretinal injections of AAV2.COMP-Ang1 or control (AAV2.AcGFP, AAV2.LacZ, and phosphate-buffered saline).
Fam83h null mice support a neomorphic mechanism for human ADHCAI.
Simmer et al., Montréal, Canada. In Mol Genet Genomic Med, Jan 2016
We generated and characterized Fam83h-knockout/lacZ-knockin mice.
The Origin of Mutants Under Selection: How Natural Selection Mimics Mutagenesis (Adaptive Mutation).
Roth et al., Davis, United States. In Cold Spring Harb Perspect Biol, Jul 2015
This provides sufficient LacZ activity to support plasmid replication but not cell division.
[Role of the sweet taste receptor in glucose metabolism: no sweets for diabetes?].
Kawahara et al., In Yakugaku Zasshi, 2014
To clarify the physiological roles of T1R2 in glucose homeostasis, T1r2-lacZ knock-in/knock-out mice were generated.
A correlation study on the effects of DNMT1 on methylation levels in CD4(+) T cells of SLE patients.
Xu et al., Shanghai, China. In Int J Clin Exp Med, 2014
and a control plasmid (pLenti6.3/V5-GW/LacZ)
Maternal uniparental isodisomy causing autosomal recessive GM1 gangliosidosis: a clinical report.
Schmidt et al., Washington, D.C., United States. In J Genet Couns, 2014
Lysosomal enzyme testing identified an isolated deficiency of beta-galactosidase.
[Neuronal ageing].
Sunderland et al., In Postepy Biochem, 2013
Some markers of senescence, whose one of them is SA-beta-galactosidase were used to show the process of neuronal ageing both in vitro, and in vivo.
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.
Clevers et al., Utrecht, Netherlands. In Nature, 2013
Here we show that Lgr5-lacZ is not expressed in healthy adult liver, however, small Lgr5-LacZ(+) cells appear near bile ducts upon damage, coinciding with robust activation of Wnt signalling.
[What will happen to molecular cell biomarkers of aging in case we cancel its program (of course, if it does exist)?].
Khokhlov, In Adv Gerontol, 2012
In addition, there are reasons to believe that the biomarkers of aging, proposed by these studies (and in particular, the most popular of them--the activity of senescence-associated beta-galactosidase), are related, as a rule, to the proliferative status of the cells, which in the whole body is generally determined by proper implementing the program of development and differentiation, leading to the emergence of tissues and organs composed of postmitotic or very slowly proliferating cells.
IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia.
Colonna et al., Saint Louis, United States. In Nat Immunol, 2012
Through the use of IL-34-deficient (Il34(LacZ/LacZ)) reporter mice, we found that keratinocytes and neurons were the main sources of IL-34.
Substitution for Asn460 cripples β-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability.
Huber et al., Calgary, Canada. In Arch Biochem Biophys, 2012
Substrate affinity to N460D-beta-galactosidase was enhanced through greater binding enthalpy (stronger H-bonds through the bridging water) while better affinity to N460T-beta-galactosidase occurred because of greater binding entropy.
Disulfide bond formation and activation of Escherichia coli β-galactosidase under oxidizing conditions.
García-Fruitós et al., Barcelona, Spain. In Appl Environ Microbiol, 2012
study demonstrate an enhancement of beta-galactosidase activity when the enzyme is produced in an oxidizing environment and, consequently, the formation of a disulfide bond in E. coli beta-galactosidase under these conditions
Effects of Mg(2+) on in vivo transcriptional dynamics of the lar promoter.
Ribeiro et al., Tampere, Finland. In Biosystems, 2012
Results on lar, a lac promoter variant indicate the increase as well as the decrease in RNA production with increasing Mg2+ concentration; these changes are, at least partially, caused by changes in the dynamics of formation of the open complex, during transciption initiation.
Ser-796 of β-galactosidase (Escherichia coli) plays a key role in maintaining a balance between the opened and closed conformations of the catalytically important active site loop.
Huber et al., Calgary, Canada. In Arch Biochem Biophys, 2012
the hydrophobic and H-bonding properties of Ser-796 result in interactions strong enough to stabilize the open or closed conformations of the loop but weak enough to allow loop movement during the reaction
Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events.
Lovett et al., Waltham, United States. In Genetics, 2011
Studies reengineered reporters that detect specific base substitutions or frameshifts in lacZ with the reverting lacZ locus on the chromosome rather than an F' element.
Imaging chromophores with undetectable fluorescence by stimulated emission microscopy.
Xie et al., Cambridge, United States. In Nature, 2009
We demonstrate a variety of applications of stimulated emission microscopy, such as visualizing chromoproteins, non-fluorescent variants of the green fluorescent protein, monitoring lacZ gene expression with a chromogenic reporter, mapping transdermal drug distributions without histological sectioning, and label-free microvascular imaging based on endogenous contrast of haemoglobin.
A crucial role for adipose tissue p53 in the regulation of insulin resistance.
Komuro et al., Chiba, Japan. In Nat Med, 2009
We found that excessive calorie intake led to the accumulation of oxidative stress in the adipose tissue of mice with type 2 diabetes-like disease and promoted senescence-like changes, such as increased activity of senescence-associated beta-galactosidase, increased expression of p53 and increased production of proinflammatory cytokines.
Effects of antibiotics and a proto-oncogene homolog on destruction of protein translocator SecY.
Silhavy et al., Princeton, United States. In Science, 2009
LacZ hybrid proteins have long been used to study translocation in Escherichia coli.
More papers using lacZ antibodies
Type I interferon signaling is required for activation of the inflammasome during Francisella infection.
Mokrousov Igor, In PLoS ONE, 2006
... lacZVector based on pQE30 (Qiagen); T5lacO; ColE1 replicon; Cam ...
Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila
O'Farrell Patrick H, In PLoS Biology, 2006
[20] (we screened a battery of transgenic LacZ reporter flies carrying ...
High efficiency site-specific genetic engineering of the mosquito genome
Arcà Bruno et al., In BMC Research Notes, 2005
... LacZ expression analysis in transgenic adult mosquitoes ...
Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions.
Lobaccaro Jean-Marc A., In PLoS ONE, 2004
... TCF/Lef-LacZ transgenic mice were a kind gift ...
eIF4E is a central node of an RNA regulon that governs cellular proliferation
Borden Katherine L.B. et al., In The Journal of Cell Biology, 2004
... For the TetON system, chimeric lacZ constructs were cloned into pTREMyc vector (CLONTECH Laboratories, Inc.) using EcoRI ...
Hydrocephalus due to diffuse villous hyperplasia of the choroid plexus. Case report and review of the literature
Mohanraj Rajesh, In PLoS ONE, 2003
... JAM-C deficient mice produced by insertion of an IRES-LacZ-pA cassette and neomycin resistance cassette and transgenic mice with endothelial-specific overepression ...
JunB is required for endothelial cell morphogenesis by regulating core-binding factor β
Schorpp-Kistner Marina et al., In The Journal of Cell Biology, 2003
... -lacZ (Schlaeger et al., 1997) transgenic mice were provided by ...
Transmission of mutations in the lacI transgene to the offspring of ENU-treated Big Blue male mice
Seligy Vern L. et al., In Mutagenesis, 2001
... lacZ DNA per diploid genome were made by mixing 1 μg of non-transgenic mouse CD2F ...
Novel chemiluminescence assay for serum periostin levels in women with preeclampsia and in normotensive pregnant women
Zwijsen An et al., In Stem Cells International, 2001
... BRE : LacZ embryos are transgenic for a gene composed of ...
Astrocyte inactivation of the pRb pathway predisposes mice to malignant astrocytoma development that is accelerated by PTEN mutation.
Castro Maria G., In PLoS ONE, 2001
... acidic protein promoter; hPDGFAl, human platelet-derived growth factor A long; IRES, internal ribosome entry site; βGEO, beta-galactosidase/neomycine fusion; pA, polyadenylation signal.)
Myogenic specification of side population cells in skeletal muscle
Rudnicki Michael A. et al., In The Journal of Cell Biology, 2000
... MD6.0-lacZ (Asakura et al., 1995) transgenic mice were provided from ...
Aging alters the apoptotic response to genotoxic stress.
Chen David J., In PLoS ONE, 2000
... mice on a C57/BL/6J background were crossed with C57BL/6J pUR288-(lacZ)-transgenic mice line 60 (integration ...
A computational approach to identify genes for functional RNAs in genomic sequences.
Marinus Martin G., In PLoS ONE, 2000
... , ER2566 (lacZ::T7 gene1) (New England Biolabs, Ipswich, MA, USA), DM49 ...
Requirement of Bmp8b for the generation of primordial germ cells in the mouse.
Abdelhay Eliana Saul Furquim Werneck, In PLoS ONE, 1999
... The female ESC line ES Rosa26 was generated from Rosa26-LacZ transgenic mouse line as described ...
Toll-like receptor family and signalling pathway
Dangl Jeffery L, In PLoS Biology, 1999
... purified plasmid DNAs from AD vectors and BD vectors were cotransformed into the yeast cell pEGY48/p8op-LacZ (Clontech) using the Yeast transformation ...
Virulence role of V antigen of Yersinia pestis at the bacterial surface.
Nilles Matthew L et al., In BMC Microbiology, 1998
... -lacZClontech ...
Ku70: a candidate tumor suppressor gene for murine T cell lymphoma
van Steeg Harry et al., In Pathobiology of Aging & Age Related Diseases, 1997
... pUR288(lacZ) transgenic line 30 mice ( ...
The human ZBP-89 homolog, located at chromosome 3q21, represses gastrin gene expression
Leder Philip et al., In The Journal of Experimental Medicine, 1997
... ) was subcloned into the HindIII site of the promoterless LacZ (Escherichia coli β-galactosidase) reporter vector pβgal-Basic (Clontech ...
G protein β interacts with the glucocorticoid receptor and suppresses its transcriptional activity in the nucleus
Chrousos George P. et al., In The Journal of Cell Biology, 1997
... pOP8-LacZ was purchased from CLONTECH Laboratories, Inc ...
Disruption of a putative SH3 domain and the proline-rich motifs in the 53-kDa substrate of the insulin receptor kinase does not alter its subcellular localization or ability to serve as a substrate
Ahmed Sohail et al., In The Journal of Cell Biology, 1997
... histidine and in the presence of 25 mM 3-AT were streaked out and assayed for LacZ (β-galactoside) activity using X-Gal (Life Technologies).
Molecular interactions in cell adhesion complexes
Aota Shin-ichi et al., In The Journal of Cell Biology, 1996
... -lacZ) was transformed first with the bait plasmid, pGB410, and subsequently with a human placenta cDNA library (Clontech ...
A concentrated and stable aerosol formulation of cationic lipid:DNA complexes giving high-level gene expression in mouse lung
Alton Eric WFW et al., In Respiratory Research, 1996
... Synthetic siRNAs (CAT, GFP, LacZ) were synthesized by Xeragon using 29-O-(tri-isopropyl) silyloxymethyl chemistry (Qiagen Inc ...
Mutations affecting somite formation and patterning in the zebrafish, Danio rerio.
VijayRaghavan K. et al., In The Journal of Cell Biology, 1995
... MHC-lacZ transgenic strain contains the regulatory ...
Endogenous beta-galactosidase activity in the larval, pupal, and adult stages of the fruit fly, Drosophila melanogaster, indicates need for caution in lacZ fusion-gene studies
Monesi Nadia et al., In BMC Molecular Biology, 1995
... BhC4-1-lacZ mRNA expression in an independent (-57/+40) transgenic line ...
Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos
Ekker Marc et al., In BMC Evolutionary Biology, 1992
... LacZ expression in a stable transgenic line under MmURE2 regulation ...
The regulation of myogenin gene expression during the embryonic development of the mouse.
Svensson Erik I., In PLoS ONE, 1992
... lacZ-pA reporter construct on its own does not have tissue-specific activity in transgenic mice ...
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Zwaka Thomas, In PLoS ONE, 1992
... 1151-Duf-lacZ and 1151-GFP are double transgenic strains generated from the ...
Ionic selectivity of Ih channels of rod photoreceptors in tiger salamanders.
Schwartz Arnold, In PLoS ONE, 1991
... lacZ reporter with enhanced green fluorescence protein (Clontech Inc, Palo Alto, CA) ...
Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitro
Dunnwald Martine et al., In The Journal of investigative dermatology, 1988
... MCS9.7-LacZ is a stable transgenic mouse strain that carries ...
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Pan Ying-Xian et al., In BMC Molecular Biology, 1986
... Comparison of tau/LacZ and tau/GFP reporter expression between two transgenic lines ...
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