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Dihydrofolate reductase

dihydrofolate reductase, DHFR
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: CAN, ACID, HAD, V1a, STEP
Papers using dihydrofolate reductase antibodies
Lipoprotein release by bacteria: potential factor in bacterial pathogenesis.
Chakravortty Dipshikha, In PLoS ONE, 1997
... Construction of expression vectors with a DHFR amplifiable markerTo obtain high level expression constructs we modified the pIRES (Clontech, Palo Alto, CA) vector ...
Human chromosomal fragile site FRA16B is an amplified AT-rich minisatellite repeat.
Albertson Donna G et al., In Genome Biology, 1996
... DHFR* into HCT116+chr3 cells, we used the Phoenix retroviral system (Orbigen, San Diego, CA, USA) ...
Papers on dihydrofolate reductase
Applications of Receptor- and Ligand-based Models in Inverse Docking Experiments: Recognition of Dihydrofolate Reductase using 7, 8-dialkyl-1,3-diaminopyrrolo[3, 2-f]quinazolines.
Pandya et al., Ahmadābād, India. In Curr Comput Aided Drug Des, Feb 2016
The present method was validated using 7,8-dialkyl-1,3-diaminopyrrolo[3,2-f]quinazolines that inhibit Candida albicans dihydrofolate reductase (DHFR) in vitro.
Whole genome sequencing of diverse Shiga toxin-producing and non-producing Escherichia coli strains reveals a variety of virulence and novel antibiotic resistance plasmids.
Feng et al., Rockville, United States. In Plasmid, Jan 2016
Among the novel plasmids identified were eight that carried resistance genes to aminoglycosides, carbapenems, penicillins, cephalosporins, chloramphenicol, dihydrofolate reductase inhibitors, sulfonamides, tetracyclines and resistance to heavy metals.
E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression.
de Bruin et al., Utrecht, Netherlands. In Methods Mol Biol, Dec 2015
Afterwards it was shown that E2F also binds to promoters of nonviral genes such as C-MYC and DHFR, which were already known at that time to be important for cell growth and DNA metabolism, respectively.
New techniques in antibiotic discovery and resistance: Raman spectroscopy.
Heidari-Torkabadi et al., Cleveland, United States. In Ann N Y Acad Sci, Sep 2015
Results are discussed for two classes of compounds inhibiting either β-lactamase or dihydrofolate reductase enzymes in a number of Gram-positive or Gram-negative cell lines.
Next-Generation Sequencing of Plasmodium vivax Patient Samples Shows Evidence of Direct Evolution in Drug-Resistance Genes.
Winzeler et al., San Diego, United States. In Acs Infect Dis, Sep 2015
In contrast we find more than three haplotypes in putative drug-resistance genes, including the gene encoding dihydrofolate reductase-thymidylate synthase and the P. vivax multidrug resistance associated transporter, suggesting that resistance mutations have arisen independently.
Cancer metabolism and oxidative stress: Insights into carcinogenesis and chemotherapy via the non-dihydrofolate reductase effects of methotrexate.
Khasawneh et al., Huntington, United States. In Bba Clin, Jun 2015
Though inhibition of dihydrofolate reductase is its best known mechanisms of action, its non-dihydrofolate reductase dependent mechanisms disrupt metabolic pathways resulting in a depletion of NAD(P)H and increasing oxidative stress.
The molecular basis of antifolate resistance in Plasmodium falciparum: looking beyond point mutations.
Kirkman et al., In Ann N Y Acad Sci, Apr 2015
The primary determinants of resistance in Plasmodium falciparum are well-described point mutations in the enzymes dihydropteroate synthase and dihydrofolate reductase targeted by the combination sulfadoxine-pyrimethamine. Recent work has highlighted the contributions of additional parasite adaptation to antifolate resistance.
Perspectives on electrostatics and conformational motions in enzyme catalysis.
Benkovic et al., United States. In Acc Chem Res, Mar 2015
We have used a combination of site-directed mutagenesis, pre-steady-state kinetics, X-ray crystallography, nuclear magnetic resonance (NMR), vibrational and fluorescence spectroscopies, resonance energy transfer, and computer simulations to study the implications of conformational motions and electrostatic interactions on enzyme catalysis in the enzyme dihydrofolate reductase (DHFR).
The importance of ensemble averaging in enzyme kinetics.
Truhlar et al., Barcelona, Spain. In Acc Chem Res, Mar 2015
We first illustrate the theory by discussing the application to both wild-type and mutant Escherichia coli dihydrofolate reductase and hyperthermophilic Thermotoga maritima dihydrofolate reductase (DHFR); DHFR is of special interest because the protein conformational changes have been widely studied.
Keep on moving: discovering and perturbing the conformational dynamics of enzymes.
Fraser et al., San Francisco, United States. In Acc Chem Res, Mar 2015
Examples are the catalytic cycles of dihydrofolate reductase (DHFR) and cyclophilin A (CypA).
Synthesis, Biological Evaluation and Molecular Docking Studies of 6-Aryl-2-Styrylquinazolin-4(3H)-Ones.
Ramasami et al., Pretoria, South Africa. In Molecules, 2014
Molecular docking studies (in silico) were conducted on compounds 5a, b, d and 6a, b, d-f to recognize the hypothetical binding motif of the title compounds within the active site of the dihydrofolate reductase and thymidylate synthase enzymes.
Frequencies distribution of dihydrofolate reductase and dihydropteroate synthetase mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium falciparum population from Hadhramout Governorate, Yemen.
Lim et al., Kuala Lumpur, Malaysia. In Malar J, 2014
This study aimed to screen mutations in the dihydrofolate reductase (dhfr) and dihydropteroate synthetase (dhps) genes associated with SP resistance among P. falciparum population in Hadhramout governorate, Yemen.
Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria.
Kato et al., Hiroshima, Japan. In Subcell Biochem, 2014
In particular, the backbone structure of the deep-sea DHFR from Moritella profunda (mpDHFR) almost overlapped with the normal homolog from Escherichia coli (ecDHFR).
Protein conformational populations and functionally relevant substates.
Agarwal et al., Oak Ridge, United States. In Acc Chem Res, 2014
For example, the conformational landscape of the enzyme dihydrofolate reductase has multiple substates, which facilitate the binding and the release of the cofactor and substrate and catalyze the hydride transfer.
Automated identification of functional dynamic contact networks from X-ray crystallography.
Fraser et al., Stanford, United States. In Nat Methods, 2013
Contact networks determined for Escherichia coli dihydrofolate reductase (ecDHFR) predict the observed long-range pattern of NMR chemical shift perturbations of an allosteric mutation.
Association of dihydrofolate reductase (DHFR) -317AA genotype with poor response to methotrexate in patients with rheumatoid arthritis.
Krajinovic et al., Belgrade, Serbia. In Clin Exp Rheumatol, 2012
Rheumatoid arthritis patients with DHFR-317AA genotype had less favourable response to methotrexate.
Protein unfolding and degradation by the AAA+ Lon protease.
Sauer et al., Beersheba, Israel. In Protein Sci, 2012
Robust and processive unfolding/degradation of some substrates with very stable protein domains, including mDHFR and titin(I27) .
Identification of endogenous ligands bound to bacterially expressed human and E. coli dihydrofolate reductase by 2D NMR.
Wright et al., Los Angeles, United States. In Febs Lett, 2011
human dihydrofolate reductase is bound to NADP.
Importance of gene variants and co-factors of folate metabolic pathway in the etiology of idiopathic intellectual disability.
Mukhopadhyay et al., Calcutta, India. In Nutr Neurosci, 2011
Constituents of the folate cycle could be involved in the etiology of idiopathic intellectual disability.
Molecular monitoring of resistant dhfr and dhps allelic haplotypes in Morogoro and Mvomero districts in south eastern Tanzania.
Roper et al., Morogoro, Tanzania. In Afr Health Sci, 2011
This report has clearly shown that the population rate of change of resistant dhfr and dhps alleles is contingent to the sulfadoxine-pyrimethamine usage in the population in the Morogor-Mvomero district
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