In vitro chromatin templates to study nucleotide excision repair.
West Lafayette, United States. In Dna Repair (amst), Dec 2015
At least three systems have been used to analyze the effect of nucleosome folding on nucleotide excision repair (NER) in vitro: (a) human cell extracts that have to rely on labeling of repair synthesis to monitor DNA repair, due to very low repair efficacy; (b) Xenopus oocyte nuclear extracts, that have very robust DNA repair efficacy, have been utilized to follow direct removal of DNA damage; (c) six purified human DNA repair factors (RPA, XPA, XPC, TFIIH, XPG, and XPF-ERCC1) that have been used to reconstitute excision repair in vitro.
eNERgizing pluripotent gene transcription.
Boston, United States. In Cell Stem Cell, 2011
(2011) in the most recent issue of Cell demonstrates that the XPC/RAD23B/CETN2 nucleotide excision repair complex additionally functions as a transcriptional coactivator of Oct4/Sox2, critically regulating maintenance and reestablishment of stem cell pluripotency.