Selection and evaluation of reference genes for expression studies with quantitative PCR in the model fungus Neurospora crassa under different environmental conditions in continuous culture.
Washington, D.C., United States. In Plos One, 2013
Based on combined stability values from NormFinder and Best Keeper software packages, the following are the most appropriate reference genes under conditions of: (1) light/dark cycling: btl, asl, and vma1; (2) all-dark growth: btl, tbp, vma1, and vma2; (3) temperature flux: btl, vma1, act, and asl; (4) all conditions combined: vma1, vma2, tbp, and btl.
A new fusion protein platform for quantitatively measuring activity of multiple proteases.
Hefei, China. In Microb Cell Fact, 2013
RESULTS: We tested seven different protease cleavage sequences (rhinovirus 3C, TEV protease, factor Xa, Ssp DnaB intein, Sce VMA1 intein, thrombin and enterokinase), three different tags (His6, GST, CBD and MBP) and two different DALs (eDAL and sDAL), for their performance as substrate to the seven corresponding proteases.
Reflections on protein splicing: structures, functions and mechanisms.
Tokyo, Japan. In Proc Jpn Acad Ser B Phys Biol Sci, 2008
VMA1 consists of a single open reading frame and contains two independent genetic information for Vma1p (a catalytic 70-kDa subunit of the vacuolar H(+)-ATPase) and VDE (a 50-kDa DNA endonuclease) as an in-frame spliced insert in the gene.
Protein splicing: its chemistry and biology.
Tokyo, Japan. In Genes Cells, 1997
This unique autocatalytic reaction was first discovered in the yeast VMA1 protein, a 120kDa spliced polypeptide encoded by the VMA1 gene of Saccharomyces cerevisiae.
Protozyme: emerging evidence in nature.
Tokyo, Japan. In J Biochem, 1994
The VMA1 locus in Saccharomyces cerevisiae contains a nested genetic element, the VDE gene, and expresses two functional proteins.