ARL4, an ARF-like Protein That Is Developmentally Regulated and Localized to Nuclei and Nucleoli
In PLoS ONE, 1999
... ATPase, Bax (Santa Cruz Biotechnology, Santa Cruz, CA, USA), VDAC (Cell Signaling Technology, Danvers, MA, USA), ...
Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells.
Edmonton, Canada. In Methodsx, 2014
simple to replicate or adjust; this method does not require expensive reagents or use of commercial kits•The protocol can be applied to tissue samples or cultured cells without changing buffer components•Yields purified fractions of cytosolic, membrane bound and nuclear proteins, with the proper distribution of the appropriate subcellular markers: GAPDH, VDAC, SERCA2 and lamin A/C.