Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
Guangzhou, China. In Stem Cells Dev, May 2015
Cell proliferation, multilineage differentiation, and the expression of Oct4B1, Sox2, Nanog, Klf4, c-Myc, and Utf1 in DPCs after Oct4A transfection were detected by cell counting kit-8, Alizarin red/Oil red O staining, immunofluorescent staining, alkaline phosphatase analysis, and real-time PCR.
Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity.
Århus, Denmark. In Stem Cell Res Ther, 2014
The iPSC-MSCs and the tri-lineage differentiated cells (osteoblasts, chondrocytes, adipocytes) all lack expression of "stemness" genes: OCT4, SOX2, GDF3, CRIPTO, UTF1, DPPA4, DNMT3B, LIN28a, and SAL4.
Reprogramming barriers and enhancers: strategies to enhance the efficiency and kinetics of induced pluripotency.
Yazd, Iran. In Cell Regen (lond), 2014
Different strategies have been applied for enhancing reprogramming efficiency, including depletion/inhibition of barriers (p53, p21, p57, p16(Ink4a)/p19(Arf), Mbd3, etc.), overexpression of enhancing genes (e.g., FOXH1, C/EBP alpha, UTF1, and GLIS1), and administration of certain cytokines and small molecules.
Utf1: Goldilocks for ESC bivalency.
Sacramento, United States. In Cell Stem Cell, 2013
Recently in Cell, Jia et al. (2012) reported novel Utf1-controlled mechanisms of maintaining pluripotency and self-renewal in embryonic stem cells (ESCs).