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UPF3 Upf3p

UPF3, Upf3p, hUpf3a
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: caspase-3, UPF1, UPF2, CAN, ACID
Papers on UPF3
Human nonsense-mediated mRNA decay factor UPF2 interacts directly with eRF3 and the SURF complex.
New
Llorca et al., Madrid, Spain. In Nucleic Acids Res, Feb 2016
According to prevailing models, NMD begins by the assembly of the SURF (SMG1-UPF1-eRF1-eRF3) complex at the ribosome, followed by UPF1 activation by additional factors such as UPF2 and UPF3.
Unique Aspects of Plant Nonsense-Mediated mRNA Decay.
Review
New
Shaul, Ramat Gan, Israel. In Trends Plant Sci, Nov 2015
However, while the loop regulating UPF3 (up-frameshift 3) expression in not vital for mammalian NMD, the sensitivity of UPF3 to NMD is crucial for the overall regulation of plant NMD.
Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.
New
Wieslander et al., Stockholm, Sweden. In J Cell Biol, Nov 2015
The EJC core does not recruit the nonsense-mediated decay mediaters UPF2 and UPF3 until the BR messenger RNA protein complexes (mRNPs) enter the interchromatin.
The feedback control of UPF3 is crucial for RNA surveillance in plants.
New
Shaul et al., Ramat Gan, Israel. In Nucleic Acids Res, May 2015
The Arabidopsis NMD factor UPF3 is regulated by a negative feedback-loop that targets its own transcript for NMD.
An UPF3-based nonsense-mediated decay in Paramecium.
Villalobo et al., Sevilla, Spain. In Res Microbiol, 2014
One of its components, UPF3, is a molecular link bridging through its binding to the exon junction complex nonsense-mediated decay and splicing.
Cbc2p, Upf3p and eIF4G are components of the DRN (Degradation of mRNA in the Nucleus) in Saccharomyces cerevisiae.
Das et al., Calcutta, India. In Fems Yeast Res, 2014
In this study, we further identify three novel components of DRN (Cbc2p, Upf3p and Tif4631p) by employing a genetic screen and by considering proteins/factors that interact with Cbc1p.
Defining reference genes for quantitative real-time PCR analysis of anther development in rice.
Li et al., Wuhan, China. In Acta Biochim Biophys Sin (shanghai), 2014
UPF3, eIF4A-3, GAPDH, and PPP6 were identified as the most suitable reference genes for qPCR analysis of anther development in rice.
Phosphorylation of the N- and C-terminal UPF1 domains plays a critical role in plant nonsense-mediated mRNA decay.
Silhavy et al., Gödöllő, Hungary. In Plant J, 2013
If termination is slow, the UPF1 NMD factor binds the eRF3 protein of the termination complex and then recruits UPF2 and UPF3.
Nonsense-mediated decay of alternative precursor mRNA splicing variants is a major determinant of the Arabidopsis steady state transcriptome.
Wachter et al., Tübingen, Germany. In Plant Cell, 2013
To address this, we conducted transcriptome-wide splicing studies using Arabidopsis thaliana mutants in the NMD factor homologs UP FRAMESHIFT1 (UPF1) and UPF3 as well as wild-type samples treated with the translation inhibitor cycloheximide.
Arabidopsis plants having defects in nonsense-mediated mRNA decay factors UPF1, UPF2, and UPF3 show photoperiod-dependent phenotypes in development and stress responses.
GeneRIF
Wu et al., Jena, Germany. In J Integr Plant Biol, 2012
The involvement of UPF1, UPF2, and UPF3 in development and in response to stresses, wounding and infection by Pseudomonas syringae pv. tomato strain DC3000, were examined.
Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense.
GeneRIF
Shin et al., Seoul, South Korea. In Plant Cell Physiol, 2011
UPF1 and UPF3, which are key nonsense-mediated mRNA decay factors, may act as defense-related regulators associated with plant immunity.
Plant serine/arginine-rich proteins: roles in precursor messenger RNA splicing, plant development, and stress responses.
Review
Shad Ali et al., Fort Collins, United States. In Wiley Interdiscip Rev Rna, 2011
Most SR splice variants contain a premature termination codon and are degraded by up-frameshift 3 (UPF3)-mediated nonsense-mediated decay (NMD), suggesting a link between NMD and regulation of expression of the functional transcripts of SR proteins.
Extensive coupling of alternative splicing of pre-mRNAs of serine/arginine (SR) genes with nonsense-mediated decay.
GeneRIF
Reddy et al., Fort Collins, United States. In New Phytol, 2010
Here, we analyzed the abundances of all splice variants for each alternatively spliced gene in an Arabidopsis mutant that lacks UPF3, one of the core components of NMD machinery, to determine if the PTC-containing transcripts are degraded by NMD.
A UPF3-mediated regulatory switch that maintains RNA surveillance.
GeneRIF
Wilkinson et al., Houston, United States. In Nat Struct Mol Biol, 2009
Results suggest that UPF3A levels are tightly regulated by a post-transcriptional switch to maintain appropriate levels of NMD substrates in cells containing different levels of UPF3B.
Aberrant mRNA transcripts and the nonsense-mediated decay proteins UPF2 and UPF3 are enriched in the Arabidopsis nucleolus.
GeneRIF
Brown et al., Dundee, United Kingdom. In Plant Cell, 2009
The nonsense-mediated decay factor UPF3 localize to the nucleolus, suggesting that the Arabidopsis nucleolus is therefore involved in identifying aberrant mRNAs and NMD.
UPF1 P-body localization.
Review
Wen et al., Birmingham, United Kingdom. In Biochem Soc Trans, 2008
However, recent studies have indicated that the core NMD factors UPF1 (up-frameshift-1), UPF2 and UPF3 can associate with P-bodies (processing bodies), which are large cytoplasmic granules replete with proteins involved in general mRNA decay and related processes.
Mutations in UPF3B, a member of the nonsense-mediated mRNA decay complex, cause syndromic and nonsyndromic mental retardation.
Impact
Gécz et al., Cambridge, United Kingdom. In Nat Genet, 2007
By systematically sequencing 737 genes (annotated in the Vertebrate Genome Annotation database) on the human X chromosome in 250 families with X-linked mental retardation, we identified mutations in the UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B) leading to protein truncations in three families: two with the Lujan-Fryns phenotype and one with the FG phenotype.
Targeting of aberrant mRNAs to cytoplasmic processing bodies.
Impact
Parker et al., Tucson, United States. In Cell, 2006
Upf1p is sufficient for targeting mRNAs to P-bodies, whereas Upf2p and Upf3p act, at least in part, downstream of P-body targeting to trigger decapping.
Transcript selection and the recruitment of mRNA decay factors for NMD in Saccharomyces cerevisiae.
Review
Neeno-Eckwall et al., In Rna, 2005
In Saccharomyces cerevisiae, nonsense-mediated mRNA decay (NMD) requires Upf1p, Upf2p, and Upf3p to accelerate the decay rate of two unique classes of transcripts: (1) nonsense mRNAs that arise through errors in gene expression, and (2) naturally occurring transcripts that lack coding errors but have built-in features that target them for accelerated decay (error-free mRNAs).
Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon.
Impact
Steitz et al., New Haven, United States. In Cell, 2001
We describe three novel human proteins involved in NMD, hUpf2, hUpf3a, and hUpf3b.
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