Using Phos-Tag in Western Blotting Analysis to Evaluate Protein Phosphorylation.
Japan. In Methods Mol Biol, Dec 2015
Here, we describe our method, which is based on in vitro kinase assay and Western blotting analysis using biotinylated Phos-tag and horseradish peroxidase-conjugated streptavidin, to determine the sites of TRPC6 (transient receptor potential canonical 6) channel phosphorylated by protein kinase A.
Melanopsin signalling in mammalian iris and retina.
Baltimore, United States. In Nature, 2011
Ablating in mouse the expression of both TRPC6 and TRPC7, members of the TRP channel superfamily, also essentially eliminated the M1-ipRGC light response but the intrinsic PLR was not affected.