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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

PR domain containing 10

The protein encoded by this gene is a transcription factor that contains C2H2-type zinc-fingers. It also contains a positive regulatory domain, which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: CAN, ACID, fibrillin-1, SDSL, V1a
Papers using Tris antibodies
Srt1720, Srt2183, Srt1460, and resveratrol are not direct activators of SIRT1
Todd Matthew H., In PLoS ONE, 2009
... Human serum albumin (HSA) and TTN buffer (0.05 M Tris, pH 7.5, 0.3 M NaCl, 1% v/v Tween 20) were from Statens Serum Institut (Copenhagen, Denmark) ...
Interacting physical, chemical and biological forcing of phytoplankton thin-layer variability in Monterey Bay, California.
Zhao Zhongming, In PLoS ONE, 2009
... probes (139 ng/uL in 0.28 mg/µL strepavidin (Sigma), 497 mM NaCl, 3 mM EDTA, 100 mM Tris-HCl, pH 7.4) were printed on Optitran BAS83 (Whatman, [33]) using a non-contact microarray printer (Piezorray, PerkinElmer, Downers Grove, IL) ...
Differential regulation of full-length genome and a single-stranded 7S DNA along the cell cycle in human mitochondria.
Larsson Nils-Göran, In PLoS Genetics, 2009
... P-labeled dATP or dGTP were used, DNA was dissolved in 20 mM Tris-acetate, 50 mM potassium acetate, 10 mM magnesium acetate, and 1 mM dithiothreitol, pH 7.9, and digested with AccI (New England Biolabs, Ipswich, MA) ...
DNA interaction with PtCl2(LL) (LL = Chelating diamine ligand: N, N-dimethyltrimethylendiamine) complex
Sourinejad Noorkaram et al., In Bioinorganic Chemistry and Applications, 2008
... , and Tris-HCl were purchased from Merck.
A robust pathway for protocell growth and division under plausible prebiotic conditions
Ehrenfreund Pascale et al., In Origins of Life and Evolution of the Biosphere, 2008
... acid, 1-decanol, pyrene, 1-hydroxypyrene, 9-anthracene carboxylic acid, 1-pyrene carboxaldehyde, 9-fluorenone, 1,4 chrysene quinone and 1 M Tris buffered solution (pH 7.5) were obtained from Sigma Aldrich.
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Papers on Tris
Tris-acetate polyacrylamide gradient gel electrophoresis for the analysis of protein oligomerization.
Rosa et al., Barcelona, Spain. In Anal Bioanal Chem, Feb 2016
We combined the use of a crosslinking reagent for sample preparation, such as glutaraldehyde, with the analysis of oligomers by Tris-acetate polyacrylamide gel electrophoresis.
Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus.
Harley et al., Washington, D.C., United States. In Int J Pediatr Otorhinolaryngol, Jan 2016
Each tissue sample was extracted with MSD Tris Lysis Buffer and protein lysates were analyzed for CD 19, B-Cell Activating Factor and B-Cell Activating Receptor by western blot methods.
Tris-hydroxymethyl-aminomethane enhances capsaicin-induced intracellular Ca(2+) influx through transient receptor potential V1 (TRPV1) channels.
Uezono et al., Sakura, Japan. In J Pharmacol Sci, Jan 2016
Tris-hydroxymethyl aminomethane (THAM) is widely used as an alkalizing agent; however, the effects of THAM on TRPV1 channels have not been defined.
Experimental Genome-Wide Determination of RNA Polyadenylation in Chlamydomonas reinhardtii.
Hunt et al., Frankfort, United States. In Plos One, Dec 2015
Specifically, PAT-Seq was used to study poly(A) site choice in cultures grown in four different media types-Tris-Phosphate (TP), Tris-Phosphate-Acetate (TAP), High-Salt (HS), and High-Salt-Acetate (HAS).
Acute Exposure to Tris(1,3-dichloro-2-propyl) Phosphate (TDCIPP) Causes Hepatic Inflammation and Leads to Hepatotoxicity in Zebrafish.
Gong et al., Wuhan, China. In Sci Rep, Dec 2015
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in environmental media and has adverse health effect on wildlife and humans.
Microenvironmental acidosis in carcinogenesis and metastases: new strategies in prevention and therapy.
Gatenby et al., Roma, Italy. In Cancer Metastasis Rev, 2014
This may be achieved with existing molecules such as proton pump inhibitors (PPIs) and buffers such as sodium bicarbonate, citrate, or TRIS.
Efficacy of behavioural interventions for transport behaviour change: systematic review, meta-analysis and intervention coding.
Araujo-Soares et al., Newcastle upon Tyne, United Kingdom. In Int J Behav Nutr Phys Act, 2013
Searches were conducted on all records in Applied Social Sciences Index and Abstracts (ASSIA), Ovid Embase, Ovid Medline, Ovid PsycInfo, Scopus, Sociological Abstracts, Transportation Research Information Service (TRIS), Transportation Research International Documentation (TRID), and Web of Science databases.
Complex formation of cadmium with sugar residues, nucleobases, phosphates, nucleotides, and nucleic acids.
Sigel et al., Zürich, Switzerland. In Met Ions Life Sci, 2012
With buffers like Tris or Bistris mixed ligand complexes are formed.
Leung, Bethesda, United States. In Unknown Journal, 2012
Mishra et al. (4) prepared (99m)Tc-1,4,7-tris(carboxymethyl)-10-(4-aminoethyl)-1,4,7,10-tetraazacyclododecane-folate ((99m)Tc-DO3A-EA-Folate) as a new (99m)Tc-based folate conjugate for SPECT imaging studies.
(111)In-2-(4,7,10-Tris(carboxymethyl)-1,4,7,10-tetracyclododecan-1-yl)pentanedioic acid-Trastuzumab
Leung, Bethesda, United States. In Unknown Journal, 2012
Moreau et al. (18) conjugated 2-(4,7,10-tris(carboxymethyl)-1,4,7,10-tetracyclododecan-1-yl)pentanedioic acid (DOTAGA), which is a derivative of 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA), to trastuzumab and labeled it with (111)In as (111)In-DOTAGA-trastuzumab for single-photon emission computed tomography (SPECT) imaging of HER2 expression in tumors.
Expression patterns of PRDM10 during mouse embryonic development.
Kim et al., Ch'unch'ŏn, South Korea. In Bmb Rep, 2010
PRDM10 might be a transcriptional regulator for normal tissue differentiation during mouse embryonic development.
Gd-hydroxypyridinone (HOPO)-based high-relaxivity magnetic resonance imaging (MRI) contrast agents.
Raymond et al., Berkeley, United States. In Acc Chem Res, 2009
Tris-bidentate HOPO-based ligands developed in our laboratory were designed to complement the coordination preferences of Gd(3+), especially its oxophilicity.
A trivalent system from vancomycin.D-ala-D-Ala with higher affinity than avidin.biotin.
Whitesides et al., Cambridge, United States. In Science, 1998
Tris(vancomycin carboxamide) binds a trivalent ligand derived from D-Ala-D-Ala with very high affinity: dissociation constant (Kd) approximately 4 x 10(-17) +/- 1 x 10(-17) M. High-affinity trivalent binding and monovalent binding are fundamentally different.
Electron microscopic visualization of recA-DNA filaments: evidence for a cyclic extension of duplex DNA.
Griffith et al., In Cell, 1982
As visualized by electron microscopy, RecA protein binds in a highly cooperative manner to single-stranded fd DNA in solutions of 0.01 M Tris (pH 7.5).
Letter: Prostaglandin-F2alpha receptor in human corpora lutea.
Sjöberg et al., In Lancet, 1974
Corpora lutea removed during surgery were suspended in a 0.01M Tris-HCL buffer; the mixture was homogenized and centrifuged at 600 g for 10 minutes, and then at 100,000 g for 60 minutes.
Growth-inhibiting action of tris(1-aziridinyl)-phosphine oxide in grasses.
Leasure et al., In Science, 1966
Tris(1-aziridinyl)-phosphine Oxide inhibits the growth of seedling grasses with concomitant accumulation of anthocyanins and exudation of sap.
More papers using Tris antibodies
A disposable, self-contained PCR chip
Michel Kristin, In PLoS ONE, 2008
... Acetonitrile, ethanol, and Tris-acetate EDTA (TAE) buffer (10×) were purchased from Sigma Aldrich and used without further ...
Kinetic characterization of hydrolysis of camel and bovine milk proteins by pancreatic enzymes
Saari Nazamid et al., In International Journal of Molecular Sciences, 2007
... Ethanol, acetic acid, sodium acetate, potassium phosphate, Tris-base and potassium chloride were obtained from Merck Co ...
Electrostatic fields near the active site of human aldose reductase: 1. New inhibitors and vibrational stark effect measurements.
Ramchandran Ramani, In PLoS ONE, 2007
... Abl:bosutinib isomer complexes were prepared by mixing Abl kinase domain (in sample buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM DTT) with a three-fold excess of bosutinib (Tocris Bioscience) or bosutinib isomer ...
An Enzyme-Based E-DNA Sensor for Sequence-Specific Detection of Femtomolar DNA Targets
Wanunu Meni, In PLoS ONE, 2007
... water), propylamine, sodium chloride, platinum wire 99.99% (76 µm diameter), 1.0 M tris (2-carboxy-ethyl) phosphine hydrochloride (TRIS buffer) of pH 7.0, were obtained from Sigma Aldrich.
Evidence of the indirect hormonal activity of prohormones using liver S9 metabolic bioactivation and an androgen bioassay
Bovee T. F. H. et al., In Analytical and Bioanalytical Chemistry, 2007
... -(+)-glucose, Titriplex III (EDTA), tris(hydroxymethyl)aminomethane (Tris), calcium chloride dehydrate, potassium chloride, sodium chloride, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, and dimethyl sulfoxide (DMSO) were purchased from Merck (Darmstadt, Germany) ...
SERS-Based Detection in an Optofluidic Ring Resonator Platform
Fan Xudong et al., In Sensors (Basel, Switzerland), 2006
... Lysis buffer consisting of Tris-HCL, Triton X-100, MgCl2, and sucrose used materials obtained from Sigma Aldrich.
Quantitative determination of lipase activity by Liquid Chromatography-Mass Spectrometry.
Netto Luis Eduardo Soares, In PLoS ONE, 2006
... For His-hLA purification, after folding protein was dialyzed first against 10 mM Tris-HCl (pH 8.0) and then against PBS, and next purified using TALON metal affinity resin (Clontech).
Key elements of bioanalytical method validation for small molecules
Jouyban Abolghasem et al., In The Scientific World Journal, 2006
... Analytical-grade ethanol, hydrochloric acid (HCl), methanol, 2-propanol, acetonitrile, and tris-(hydroxymethyl) aminomethane (Tris) were obtained from Merck (Darmstadt, Germany), terbium(III) chloride ...
The outer membrane protein OmpW forms an eight-stranded β-Barrel with a hydrophobic channel.
Santos Pedro, In PLoS ONE, 2005
... Urea, thiourea, Tris-HCl, NaCl and glycine were from Merck, India; ...
Estrogen deficiency and bone loss: An inflammatory tale
Murk Albertinka J. et al., In Toxicological Sciences, 2005
... 2,3-Bis(4-hydroxyphenyl)-propionitrile (DPN) and 4,4′,4″-(4-propyl-[1H]pyrazole-1,3,5-triyl) Tris-phenol (PPT) were purchased from Tocris Cookson Ltd (Bristol, UK) ...
Transcriptional regulation via the NF-kappaB signaling module.
Defossez Pierre-Antoine, In PLoS ONE, 2005
... washed twice and thereafter treated with 30 µl exonuclease mixture (50 mM KAc, 20 mM Tris-HAc pH 7.5, 3 mM MgAc, 1 mM DTT (Invitrogen), 5 U lambda exonuclease (New England Biolabs), 10 U phi29 DNA polymerase ...
Relationship of patients' age to histopathological features of breast tumours in BRCA1, BRCA2, and in mutation negative breast cancer families
Nevanlinna Heli et al., In Breast Cancer Research : BCR, 2004
... CK-14 (dilution 1:20; Novocastra, Newcastle upon Tyne, UK) were first pretreated in a microwave oven in Tris-EDTA (pH 9.0), incubated with the primary antibodies for 30 minutes and visualized using the Envision detection system (Dako) and LabVision autostainer (Thermo ...
Folate intake and risk of breast cancer characterized by hormone receptor status
Giles G G et al., In British Journal of Cancer, 2004
... routine dewaxing procedure was followed by heat-induced epitope retrieval with either citrate buffer pH 6 or TRIS EDTA pH 8 using a DAKO (Carpinteria, CA, USA) Pascal ...
Cationic liposomes containing mycobacterial lipids: a new powerful Th1 adjuvant system.
Cardona Pere-Joan, In PLoS ONE, 2004
... (extra pure), chloroform (extra pure) and 1 M hydrochloric acid, used to adjust pH in the Tris-buffer, were purchased from Merck (Darmstadt, Germany) ...
Conformational changes and protein stability of the pro-apoptotic protein Bax
Zeth Kornelius et al., In Journal of Bioenergetics and Biomembranes, 2004
... µl samples were mixed with 1 µl ammonium peroxydisulfate (APS, 25 mM) and 2 µl ruthenium (II) Tris-bipyridyldication (5 mM Sigma-Aldrich) in the dark and then immediately exposed to illumination with visible light (400 to 700 nm, generated by a Xenon lamp, 100 W Leica, filters: KG4, GG 395 ...
HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases.
Luban Jeremy, In PLoS Pathogens, 2004
... lysed by sonication in 500 mM NaCl, 0.5 mM PMSF, 20 mM imidazole, 50 mM Tris HCl, pH 7.4, and the pre-cleared lysate was incubated with NiNTA agarose (Qiagen).
Likelihood-enhanced fast rotation functions
Virgin Skip, In PLoS Biology, 2003
... Insect cell supernatants containing secreted CgE were buffer-exchanged into nickel-binding buffer (40 mM Tris [pH 8], 300 mM NaCl, 10 mM imidazole) and passed over a Ni-NTA agarose column (Qiagen, Valencia, California, United States) ...
Stage-specific activity of potential antimalarial compounds measured in vitro by flow cytometry in comparison to optical microscopy and hypoxanthine uptake
Avery Vicky M. et al., In The American Journal of Tropical Medicine and Hygiene, 2003
... NaOH, D-sorbitol, chloroquine diphosphate salt, artemisinin, 10× phosphate buffered saline (PBS) solution, EDTA, 0.5 M solution, Tris-HCl, saponin (from Quillaja Bark), Triton X-100, Giemsa stain, paraformaldehyde, dimethylformamide, and Petri dishes were purchased from Sigma Aldrich (St ...
Persistent activation of the Akt pathwayin head and neck squamous cell carcinoma: a potential target for UCN-01
Pinto-Júnior Décio-dos Santos et al., In Medicina Oral, Patología Oral y Cirugía Bucal, 2003
... After washing with Tris buffer (pH 7.4), the slides were incubated with the primary antibodies anti-TWIST (H-81 – Sc:15396; Santa Cruz Biotechnology, CA, USA), dilution 1:100, ...
Dnmt1 expression in pre- and postimplantation embryogenesis and the maintenance of IAP silencing
Badge Richard M. et al., In Molecular Biotechnology, 2003
... 25 mM EDTA, 20 mM Tris-HCl pH 8.0) and 1 ml of solution B (1% SDS, 12.5 mM EDTA, 10 mM Tris-HCl pH 8.0) to which 30 μl of Proteinase K solution (25 mg/ml) was added in a 15 ml Qiagen MaXtract tube ...
Immune stimulation mediated by autoantigen binding sites within small nuclear RNAs involves Toll-like receptors 7 and 8
Krieg Arthur M. et al., In The Journal of Experimental Medicine, 2003
... ODNs (Coley Pharmaceutical GmbH) and ORNs (BioSpring GmbH) were suspended in sterile, endotoxin-free Tris-EDTA (Sigma-Aldrich) or DNase- and RNase-free dH2O (Life Technologies), respectively ...
Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agent
Li Feng et al., In Molecular therapy. Nucleic acids, 2003
... (Burlingame, CA); β-actin monoclonal antibody (1:1,000): Abcam (Cambridge, MA)) at 4 °C overnight, washed in TBST (Tris-Buffered Saline Tween 20), and detected by a horseradish peroxidase-conjugated goat antirabbit secondary antibody (1:4,000) (Abcam) at room temperature for ...
Inhibition of hypoxia inducible factor 1 alpha causes oxygen-independent cytotoxicity and induces p53 independent apoptosis in glioblastoma cells
Djonov Valentin et al., In Breast Cancer Research, 2002
... in a graded series of decreasing ethanol concentration and then rinsed in Tris-buffered saline (50 mM Tris-HCl [pH 7.4] containing 100 mM sodium chloride).Immunostaining for hif-1α was performed according to the Catalyzed Signal Amplification System (Dako, Carpinteria, CA, USA), which ...
Synthesis and Ca2+-mobilizing activity of purine-modified mimics of adenophostin A: a model for the adenophostin-Ins(1,4,5)P3 receptor interaction
Potter Barry V. L. et al., In Journal of Medicinal Chemistry, 2002
... ATP, creatine phosphate, EGTA, Tris, and NaCl were provided from Sigma Aldrich.
A physiologic role for testosterone in limiting estrogenic stimulation of the breast
Bondy Carolyn et al., In Breast Cancer Research, 2002
... The slides were mounted with 0.1 M Tris and viewed in a Leica DMR fluorescent microscope ...
Efficient protein crystallization.
Bongard Josh, In PLoS ONE, 2002
... mes, phosphate buffered saline (PBS), glutamic acid (Glu), succinic acid (Succ), hepes, bicine and trizma (Tris) were purchased from Sigma Aldrich.
Expanding the genetic code
Roy Siddhartha et al., In Nucleic Acids Research, 2001
... Magnesium chloride, sodium chloride, calcium chloride, 2-mercaptoethanol, glycerol and Tris were purchased from E-Merck (India) ...
Raster3D. Photorealistic molecular graphics.
Egli Martin, In PLoS ONE, 2001
... The ammonium sulfate precipitate was resuspended in 20 mM Tris-HCl, pH 8.0 and purified by Ni-NTA chromatography following the manufacturer's recommendation (Qiagen, Valencia, CA) ...
Sensitivity of tyrosyl radical g-values to changes in protein structure: A high-field EPR study of mutants of ribonucleotide reductase.
Croft Anna Kristina, In PLoS ONE, 2000
... Commercial agents were used for chemicals (as obtained): 4(-2hydroxyethyl)-2-piperazineethanesulfonic acid (HEPES) (Sigma), tris(hydroxymethyl)-aminomethane (Tris) (Sigma), ferrous ammonium sulphate hexahydrate (Merck), ampicillin (Sigma), manganese(II) chloride ...
Flow cytometric platform for high-throughput single nucleotide polymorphism analysis.
Lee Edmund Jon Deoon et al., In Biological Procedures Online, 2000
... The oligonucleotide coupling and hybridization reagents: 2[N-Morpholino] ethanesulfonic acid (MES) and Tris-EDTA buffer were purchased from Sigma Aldrich, NaCl from Merck, 10% ...
An efficient recombination system for chromosome engineering in Escherichia coli.
Rutherford Julian, In PLoS ONE, 1999
... Blue stain, One-Step NBT-BCIP reagent, BCA protein assay reagents), Bio-Rad (Criterion PreCast 4–20% polyacrylamide gradient Tris-HCl protein gels), IDTDNA (oligonucleotides), New England Biolabs (restriction enzymes, DNA polymerase, ...
Restless legs syndrome: Evidence for nocturnal hypothalamic-pituitary-adrenal system activation.
Hochman Shawn, In PLoS ONE, 1999
... Tissue slices were then rinsed for 48 h at 4°C in 20% sucrose in Tris buffered saline (pH 7.4), frozen in −45°C isopentane and cut into 50 µm frontal sections with a cryostat (Leica).
The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucl.
Permyakov Eugene A., In PLoS ONE, 1999
... S100A9 protein solutions were prepared in 10 mM Tris-HCl buffer at pH 7.4, and dialyzed to pH 3.0 in 20 mM Glycine buffer using Slide-A-Lyzer Dialysis Cassettes (Thermo Scientific, USA) at 4 ...
Host and transmissible spongiform encephalopathy agent strain control glycosylation of PrP.
Koutsopoulos Sotirios, In PLoS ONE, 1998
... homogenates were prepared in homogenization buffer [0.01 M Tris-HCl, 0.15 M NaCl, 5 mM EDTA (pH 8.0), 1% v/v Triton X-100, 0.5% w/v sodium deoxycholate], using a FastPrep homogenizer (Thermo Scientific, Waltham, MA, U.S.A.) ...
Cloning and characterization of a cDNA encoding an A-kinase anchoring protein located in the centrosome, AKAP450
Milgram Sharon L. et al., In The Journal of Cell Biology, 1998
... Excess biotin was removed by dialysis in 10 mM Tris-HCl, pH 7.4, + 0.15 M NaCl.Biotin-RIIα was used as a probe to screen a λTriplEx human lung cDNA library (CLONTECH).
Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase.
Blagosklonny Mikhail, In PLoS ONE, 1998
... PVDF membrane, NuPage 4–12% Bis-Tris gels, hygromycin B, lipofectamine 2000, Dulbecco's modified Eagle's medium (DMEM), antibiotic-antimycotic solution and fetal bovine serum were from Invitrogen Life Technologies.
Glucocorticoids repress induction by thiazolidinediones, fibrates, and fatty acids of phosphoenolpyruvate carboxykinase gene expression in adipocytes.
Dahlman Ingrid A., In PLoS ONE, 1997
... (SDS) – polyacrylamide gel electrophoresis (PAGE) was performed using a SDS-MOPS running buffer and a Novex 4–12% Bis-Tris gel from Invitrogen Life Technologies (Carlsbad, CA, USA) ...
Activation of nuclear factor-κB is necessary for myotrophin-induced cardiac hypertrophy
Sen Subha et al., In The Journal of Cell Biology, 1997
... DME F-12, nonessential amino acids, antibiotics, Tris-borate EDTA, and PBS were purchased from Life Technologies.
A new approach to identifying genotoxic carcinogens: p53 induction as an indicator of genotoxic damage
Cannistraro Salvatore et al., In International Journal of Nanomedicine, 1997
... Human glutathione S-transferase (GST)-p53 1.23 mM in 50 mM Tris-acetate, pH 7.5, 1 mM EDTA, 20% glycerol) was purchased from Millegen (Labège, France) ...
Electrochemical studies of porphyrin interacting with DNA and determination of DNA
Hadidi Saba et al., In Bioinorganic Chemistry and Applications, 1996
... O, EDTA, Tris-HCl, NaOH, potassium acetate, and chloroform, were purchased from Merck.
Pharmacokinetics of Tityus serralatus scorpion venom determined by enzyme-linked immunosorbent assay in the rat
Droudi Alireza et al., In Journal of Venom Research, 1995
... Sodium dodecyl phosphate, Hydrogen peroxide, potassium phosphate buffer, sulforic acid, sodium sulfate, phenylenediamine and Tris-buffer were from Merck (Darmastadt, Germany) ...
Allosteric enhancement of adenosine A1 receptor binding and function using 2-amino-3-benzoylthiophenes
Müller Christa E. et al., In Purinergic Signalling, 1995
... Tris was obtained from Acros Organics (Leverkusen, Germany), DMSO was from Fluka (Switzerland), HCl was from Merck, HAT supplement from Gibco, ...
Inhibition of actin filament depolymerization by the Dictyostelium30,000-D actin-bundling protein
Chen Bin et al., In The Journal of Cell Biology, 1991
... The 6xHis-tagged small espin constructs were isolated from 50 mM Tris-HCl, 10 mM 2-mercaptoethanol, pH 8.5, extracts of frozen-thawed, sonicated bacteria by batch affinity chromatography on Ni-NTA resin (Qiagen, Chatsworth, CA) using the ...
Identification of self peptides bound to purified HLA-B27
Sali Andrej, In PLoS Biology, 1990
... The resulting solution was adjusted to 50 mM Tris (pH 7.4)/300 mM NaCl/10 mM imidazole/10% glycerol, and loaded onto an 8-ml Ni-NTA column (Qiagen, Valencia, California, United States) ...
Retinoic acid receptors and cellular retinoid binding proteins. II. Their differential pattern of transcription during early morphogenesis in mouse embryos
Uversky Vladimir N., In PLoS ONE, 1990
... The anti-rabbit IgG HRP-linked antibodies, Tris buffered saline with Tween-20 (TBST-10X), and cell lysis buffer were provided by Cell Signaling Technology Co ...
Accurate Bond and Angle Parameters for X-Ray Protein-Structure Refinement.
Driscoll Paul C., In PLoS ONE, 1990
... The reservoir solution was composed of 0.2 M KSCN, 0.1 M Bis-Tris propane pH 7.5, 20% w/v poly-ethylene glycol (PEG) 3350 (condition 64 in the Qiagen PACT suite of crystallisation ...
Synthesis, characterization, DNA binding, and cytotoxic studies of some mixed-ligand palladium(II) and platinum(II) complexes of α-diimine and amino acids
Hassani Fatemeh et al., In Bioinorganic Chemistry and Applications, 1990
... Potassium tetrachloroplatinate, 2,2′-bipyridine, highly polymerized calf thymus DNA sodium salt, and Tris-HCl buffer were obtained from Merck (Germany) ...
Isolation and properties of a metalloproteinase from buckwheat (Fagopyrum esculentum) seeds
Araújo Mariana da Silva et al., In The Scientific World Journal, 1989
... coatless dry seeds triturated and homogenized in 0.10 M Tris (purchased from Merck KGaA, Darmstadt, Germany) buffer ...
Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity.
Combs Colin, In PLoS ONE, 1986
... Tris(hydroxymethyl)-aminomethan was from Merck (Lyon, France) ...
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