Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay.
Liverpool, United Kingdom. In Front Genet, 2013
For example, we have shown that the E3 ubiquitin ligase Mule, the tumor suppressor protein ARF, and the deubiquitylation enzyme USP47 modulate DNA repair by controlling cellular levels of DNA polymerase β, and also that polynucleotide kinase phosphatase levels are controlled by ATM-dependant phosphorylation and Cul4A-DDB1-STRAP-dependent ubiquitylation.
Selective Dual Inhibitors of the Cancer-Related Deubiquitylating Proteases USP7 and USP47.
United States. In Acs Med Chem Lett, 2012
Inhibitors of the cancer-related cysteine isopeptidase human ubiquitin-specific proteases 7 (USP7) and 47 (USP47) are considered to have potential as cancer therapeutics, owing to their ability to stabilize the tumor suppressor p53 and to decrease DNA polymerase β (Polβ), both of which are potential anticancer effects.