Costantini Frank et al., In The Journal of Cell Biology, 1998
... 6.4-kb NheI-EcoRV fragment of MT–Axin–IRES2–GFP–hβG3′ was then inserted into the same restriction sites downstream of TRE2, which consisted of seven copies of the tet operator and a minimal CMV promoter (CLONTECH Laboratories, Inc.).
Vandenbol et al., Gembloux, Belgium. In Biotechnol Lett, 2007
The lack of secondary structure of the region flanking the TBC domain in TRE2 may explain why this region plays a role in the lack of GAP activity, even when a potentially functional TBC domain is present.