A thermostable transketolase evolved for aliphatic aldehyde acceptors.
Darmstadt, Germany. In Chem Commun (camb), 11 Feb 2015
Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.
Structure and functioning mechanism of transketolase.
Moscow, Russia. In Biochim Biophys Acta, Sep 2014
Transketolase, discovered independently by Racker and Horecker in 1953 (and named by Racker) , did not receive much attention until 1992, when crystal X-ray structure analysis of the enzyme from Saccharomyces cerevisiae was performed .
Engineering stereoselectivity of ThDP-dependent enzymes.
London, United Kingdom. In Febs J, Dec 2013
Both structurally different enzyme families differ also in stereoselectivity: enzymes from the decarboxylase family are predominantly R-selective, whereas those from the transketolase family are S-selective.
[Vitamin B1 (thiamine)].
Dijon, France. In Rev Prat, Oct 2013
The biological exploration of vitamin B1 status is based on the measurement of thiamine pyrophosphate concentration or of the activity of a thiamine-dependent enzyme, transketolase, in erythrocytes.
The role of thiamine in cancer: possible genetic and cellular signaling mechanisms.
Westminster, United States. In Cancer Genomics Proteomics, Jul 2013
Genetic studies have helped identify a number of factors that link thiamine to cancer, including the solute carrier transporter (SLC19) gene, transketolase, transcription factor p53, poly(ADP-ribose) polymerase-1 gene, and the reduced form of nicotinamide adenine dinucleotide phosphate.
Riboneogenesis in yeast.
Princeton, United States. In Cell, 2011
Riboneogenesis begins with synthesis, by the combined action of transketolase and aldolase, of the seven-carbon bisphosphorylated sugar sedoheptulose-1,7-bisphosphate.