The Mediator complex and transcription regulation.
Boulder, United States. In Crit Rev Biochem Mol Biol, 2013
Mediator and pol II function within the pre-initiation complex (PIC), which consists of Mediator, pol II, TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH and is approximately 4.0 MDa in size.
Human TFIID binds to core promoter DNA in a reorganized structural state.
Berkeley, United States. In Cell, 2013
This analysis revealed that TFIID coexists in two predominant and distinct structural states that differ by a 100 Å translocation of TFIID's lobe A. The transition between these structural states is modulated by TFIIA, as the presence of TFIIA and promoter DNA facilitates the formation of a rearranged state of TFIID that enables promoter recognition and binding.
TFIIA and the transactivator Rap1 cooperate to commit TFIID for transcription initiation.
Illkirch-Graffenstaden, France. In Nature, 2010
Transcription of eukaryotic messenger RNA (mRNA) encoding genes by RNA polymerase II (Pol II) is triggered by the binding of transactivating proteins to enhancer DNA, which stimulates the recruitment of general transcription factors (TFIIA, B, D, E, F, H) and Pol II on the cis-linked promoter, leading to pre-initiation complex formation and transcription.