Inhibition of the anti-apoptotic PI(3)K/Akt/Bad pathway by stress
In The Journal of Cell Biology, 1997
... For stable transfection, Rat-1/Wnt-1 cells were cotransfected with pcDNA3-flag-DN-Tcf-4, encoding the dominant-negative mutant (DN) of Tcf-4, or control empty vector and pBabe vector, containing a puromycin selectable marker, with Superfect (QIAGEN), according to the manufacturer's ...
Oncogenic Wnt/β-catenin signalling pathways in the cancer-resistant epididymis have implications for cancer research.
Yantai, China. In Mol Hum Reprod, Feb 2013
The low cancer incidence in the epididymis may be due to factors present in the human epididymis that regulate this oncogenic Wnt/β-catenin pathway, including (i) 14 of 17 secreted pathway inhibitors, (ii) the majority of the micro-RNAs known to target this pathway, (iii) plasma membrane-associated E-cadherin and CEACAM1 that anchor β-catenin, preventing its availability for nuclear entry and oncogenic transcriptional activity, (iv) the recently identified membrane-located tumourigenesis inhibitors RNF43 and ZNRF3 that mediate the degradation of the Wnt receptor components Fzds and Lrp5/6 and (v) nuclear KLF4, which competes with TCF for β-catenin, limiting its transcriptional activity and stabilizing telomeres, thereby reducing mutation incidence.
Monogenic diabetes mellitus due to defects in insulin secretion.
Switzerland. In Swiss Med Wkly, 2011
The knowledge of typical clinical features such as mode of inheritance, age at diagnosis and impaired insulin secretion, as well as genetic testing establishes the diagnosis of MODY, mitochondrial diabetes and neonatal diabetes.