Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.
Lausanne, Switzerland. In Cell, Jan 2016
We have solved crystal structures of a human Brf2-TBP complex bound to natural promoters, obtaining a detailed view of the molecular interactions occurring at Brf2-dependent Pol III promoters and highlighting the general structural and functional conservation of human Pol II and Pol III pre-initiation complexes.
Anti-transcription Factor RNA Aptamers as Potential Therapeutics.
Rochester, United States. In Nucleic Acid Ther, Nov 2015
We then review examples of unnatural RNA aptamers selected to inhibit TFs nuclear factor-kappaB (NF-κB), TATA-binding protein (TBP), heat shock factor 1 (HSF1), and runt-related transcription factor 1 (RUNX1).
ChIP-nexus enables improved detection of in vivo transcription factor binding footprints.
Kansas City, United States. In Nat Biotechnol, Apr 2015
To better map transcription factor binding genome-wide at nucleotide resolution in vivo, we have developed a robust ChIP-exo protocol called ChIP-nexus (chromatin immunoprecipitation experiments with nucleotide resolution through exonuclease, unique barcode and single ligation), which utilizes an efficient DNA self-circularization step during library preparation.
[Regulatory Genomics: Integrated Experimental and Computer Approaches].
In Genetika, Apr 2015
These include (a) an analysis of the factors affecting the affinity of TBP (TATA-binding protein) for the TATA box; (b) research on the patterns of chromatin mark distributions and their role in the regulation of gene expression; (c) a study of 3D chromatin organization; (d) an estimation of the effects of polymorphisms on gene expression via high-resolution Chip-seq and DNase-seq techniques.
Evolution and diversification of the basal transcription machinery.
More papers using
San Diego, United States. In Trends Biochem Sci, Mar 2015
Gradually it became apparent that the basal transcription machinery greatly diversified during evolution and new studies now demonstrate that diversification of the TATA-binding protein (TBP) family yielded specialized and largely independent transcription systems.