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Signal transducer and activator of transcription 3

STAT3, signal transducer and activator of transcription 3
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: Interleukin-6, V1a, CAN, Akt, HAD
Papers using STAT3 antibodies
A novel U-STAT3-dependent mechanism mediates the deleterious effects of chronic nicotine exposure on renal injury
Supplier
Goumans Marie Jose, In PLoS ONE, 2011
... Fluorescein-5-maleimide was from Pierce Biotechnology (Rockford, lL USA) and recombinant human STAT3 was from OriGene Technologies (Rockville, MD) ...
Quantitation of ursolic acid in human plasma by ultra performance liquid chromatography tandem mass spectrometry and its pharmacokinetic study.
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Jagetia Ganesh Chandra, In PLoS ONE, 2010
... Antibodies against phospho-STAT3 (Tyr 705), phospho-AKT (Ser 473), STAT3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Molecular control of endothelial cell behaviour during blood vessel morphogenesis
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Tang Yao Liang, In PLoS ONE, 2010
... LY294002 and antibodies against phosphorylated (p-) STAT3 (Tyr705) and STAT3 were from Cell Signaling Technology (Beverly, MA) ...
The use of nanoparticle-mediated targeted gene silencing and drug delivery to overcome tumor drug resistance
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Su Wu-Chou et al., In International Journal of Nanomedicine, 2009
... The following antibodies were used: anti-Stat3 (Tyr705), anti-Stat1, anti-α-tubulin (Cell Signaling Technology, Beverly, MA), anti-Stat3 (Santa Cruz Biotechnology, Santa Cruz, CA), and ...
Transcriptomic analysis of mild hypothermia-dependent alterations during endothelial reperfusion injury.
Supplier
Cheriyath Venugopalan, In PLoS ONE, 2009
... (BETHYL Laboratories, Inc), anti-cleaved PARP, anti-total- and phospho-AKT (Ser473), -ERK1/2 (Thr202/Tyr204), -STAT3, Tyr705) and horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG secondary antibody were from Cell Signaling Technology ...
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Papers on STAT3
Cryptotanshinone, a novel tumor angiogenesis inhibitor, destabilizes tumor necrosis factor-α mRNA via decreasing nuclear-cytoplasmic translocation of RNA-binding protein HuR.
New
Lu et al., Nanjing, China. In Mol Carcinog, 27 Sep 2015
Moreover, the underlying mechanism responsible for the regulation in angiogenesis by CT was partially related to the suppression of NF-κB, and STAT3 activity.
Oncostatin M Confers Neuroprotection against Ischemic Stroke.
New
Li et al., Wuhan, China. In J Neurosci, 26 Sep 2015
Mechanistically, OSM/OSMRβ activate the JAK2/STAT3 prosurvival signaling pathway.
Growth-inhibitory and chemosensitizing effects of microRNA-31 in human glioblastoma multiforme cells.
New
Wang et al., Linhai, China. In Int J Mol Med, 13 Sep 2015
UNASSIGNED: The constitutive activation of signal transducer and activator of transcription 3 (STAT3) contributes to resistance to temozolomide (TMZ) in glioblastoma multiforme (GBM).
dsRNA Released by Tissue Damage Activates TLR3 to Drive Skin Regeneration.
New
Impact
Garza et al., Baltimore, United States. In Cell Stem Cell, 06 Sep 2015
Here, we show that dsRNA, which is released from damaged skin, activates Toll-Like Receptor 3 (TLR3) and its downstream effectors IL-6 and STAT3 to promote hair follicle regeneration.
STAT3-dependent reactive astrogliosis in the spinal dorsal horn underlies chronic itch.
New
Impact
Tsuda et al., Fukuoka, Japan. In Nat Med, 31 Aug 2015
We found that reactive astrogliosis depended on the activation of signal transducer and activator of transcription 3 (STAT3).
Genome, Exome, and Targeted Next-Generation Sequencing in Neonatal Diabetes.
Review
New
Ellard et al., Exeter, United Kingdom. In Pediatr Clin North Am, 31 Aug 2015
For neonatal diabetes, the use of these technologies has resulted in the identification of 2 novel disease genes (GATA6 and STAT3) and a novel regulatory element of PTF1A, in which mutations cause pancreatic agenesis.
Chronic inflammation and the development of malignancy in the GI tract.
Review
New
Strober et al., Regensburg, Germany. In Trends Immunol, 31 Aug 2015
This secondary level is uniquely dependent on a single epithelial cell signaling pathway centered on STAT3, and it is this pathway upon which stimulation of mucosal cytokine production and microbiota effects converge.
Annexin A1 is involved in the acquisition and maintenance of a stem cell-like/aggressive phenotype in prostate cancer cells with acquired resistance to zoledronic acid.
New
Petrella et al., Salerno, Italy. In Oncotarget, 28 Aug 2015
DU145R80 show a cancer stem cell (CSC)-like signature with a high expression of CSC markers including CD44, CD133, NANOG, Snail, Oct4 and ALDH7A1 and CSC-related genes as STAT3.
Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma.
New
Impact
Chen et al., Shanghai, China. In Nat Genet, 20 Aug 2015
Recurrent mutations were most frequently located in the RNA helicase gene DDX3X (21/105 subjects, 20.0%), tumor suppressors (TP53 and MGA), JAK-STAT-pathway molecules (STAT3 and STAT5B) and epigenetic modifiers (MLL2, ARID1A, EP300 and ASXL3).
Serum hepcidin concentrations and type 2 diabetes.
Review
New
Tuomainen et al., Kuopio, Finland. In World J Diabetes, 10 Aug 2015
Some of the suggested pathways are via transcription modulator of hepcidin (STAT3); ferroportin 1 expression on the cells involved in iron transport; transmembrane protease 6 enzyme; and pro-inflammatory cytokines, interleukin (IL)-1, IL-6, tumor necrosis factor-α and IL-10.
Rhodomycin A, a novel Src-targeted compound, can suppress lung cancer cell progression via modulating Src-related pathways.
New
Chen et al., T'ai-chung-shih, Taiwan. In Oncotarget, 03 Aug 2015
Among them, rhodomycin A was the most efficient at reducing the activity and expression of Src in a dose-dependent manner, which was also the case for Src-associated proteins, including EGFR, STAT3, and FAK.
Asymmetric Action of STAT Transcription Factors Drives Transcriptional Outputs and Cytokine Specificity.
New
Impact
O'Shea et al., Bethesda, United States. In Immunity, Jun 2015
To evaluate the specificity and redundancy for these cytokines, we quantified their global transcriptomic changes and determined the relative contributions of STAT1 and STAT3 using genetic models and chromatin immunoprecipitation-sequencing (ChIP-seq) approaches.
Convergent mutations and kinase fusions lead to oncogenic STAT3 activation in anaplastic large cell lymphoma.
New
Impact
European T-Cell Lymphoma Study Group et al., Torino, Italy. In Cancer Cell, May 2015
We identified activating mutations of JAK1 and/or STAT3 genes in ∼20% of 88 [corrected] ALK(-) ALCLs and demonstrated that 38% of systemic ALK(-) ALCLs displayed double lesions.
The Complex Role of STAT3 in Viral Infections.
Review
New
Kuchipudi, Nottingham, United Kingdom. In J Immunol Res, Dec 2014
Signal transducer and activators of transcription-3 (STAT3) regulates diverse biological functions including cell growth, differentiation, and apoptosis.
Targeting Apoptosis and Multiple Signaling Pathways with Icariside II in Cancer Cells.
Review
New
Ma et al., Dalian, China. In Int J Biol Sci, Dec 2014
Icariside II has been found to induce apoptosis in various human cancer cell lines of different origin by targeting multiple signaling pathways including STAT3, PI3K/AKT, MAPK/ERK, COX-2/PGE2 and β-Catenin which are frequently deregulated in cancers, suggesting that this collective activity rather than just a single effect may play an important role in developing Icariside II into a potential lead compound for anticancer therapy.
Selective STAT3-α or -β expression reveals spliceform-specific phosphorylation kinetics, nuclear retention and distinct gene expression outcomes.
GeneRIF
Bogoyevitch et al., Melbourne, Australia. In Biochem J, 2012
results highlight STAT3beta as a significant transcriptional regulator in its own right, with additional actions to cross-regulate STAT3alpha phosphorylation and nuclear retention after cytokine stimulation
STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4(+) T cells.
GeneRIF
Egwuagu et al., Bethesda, United States. In J Biol Chem, 2012
STAT3 protein interacts with Class O Forkhead transcription factors in the cytoplasm and regulates nuclear/cytoplasmic localization of FoxO1 and FoxO3a proteins in CD4(+) T cells.
STAT3 transcription factor promotes instability of nTreg cells and limits generation of iTreg cells during acute murine graft-versus-host disease.
Impact
GeneRIF
Fowler et al., Bethesda, United States. In Immunity, 2012
STAT3 limited FoxP3+ Treg cell numbers following allogeneic bone marrow transplantation by two pathways: instability of natural Treg (nTreg) cells and inhibition of induced Treg (iTreg) cell polarization from naive CD4+ T cells.
The stat3/socs3a pathway is a key regulator of hair cell regeneration in zebrafish. [corrected].
GeneRIF
Burgess et al., Bethesda, United States. In J Neurosci, 2012
The results of this study suggested that the stat3/socs3 pathway is a key response in all tissue regeneration in zebrafish.
Activation of Stat3 in endothelial cells following hypoxia-reoxygenation is mediated by Rac1 and protein Kinase C.
GeneRIF
Becker et al., Baltimore, United States. In Biochim Biophys Acta, 2012
Stat3 forms a multiprotein complex with Rac1 and PKC in an hypoxia-reoxygenation-dependent manner.
More papers using STAT3 antibodies
STAT3 transcriptional factor activated by reactive oxygen species induces IL6 in starvation-induced autophagy of cancer cells
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Viola Joao P. B., In PLoS ONE, 2009
... Anti-STAT3 and anti-p-STAT3 (Y705) were purchased from Cell Signaling.
Persistently activated Stat3 maintains constitutive NF-kappaB activity in tumors
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Fagard Remi et al., In BMC Cell Biology, 2008
... with binding buffer, complexes were separated on SDS-polyacrylamide (8%) gel, subjected to immunoblotting using an anti-phospho-STAT3 antibody (Cell Signaling); input was determined by ...
Plasma and urine biomarkers in acute viral hepatitis E.
Supplier
Tavis John E., In PLoS ONE, 2008
... The antibodies to phospho-Akt, Akt, HNF4, PARP and STAT3 were purchased from Cell Signaling Technology (Beverly, MA, USA) ...
Notch1 signaling stimulates proliferation of immature cardiomyocytes
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Giacca Mauro et al., In The Journal of Cell Biology, 2007
... anti–GATA-4 antibodies were obtained from Santa Cruz Biotechnology, Inc.; anti–cleaved Notch-1 (Val1744), anti–phospho-p38 MAPK, and anti–phospho-STAT3 (Tyr705) were obtained from Cell Signaling Technology; antisarcomeric α-actinin (EA-53) ...
Spatially and temporally controlled electroporation of early chick embryos.
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Najbauer Joseph, In PLoS ONE, 2007
... Jak I inhibitor 0.6 µM (Calbiochem) was used to block LIF-Stat3 pathway; SB431542 10 µM (Tocris Bioscience) and rhNoggin 500 ...
Rho GTPase activation assays.
Supplier
Beaudoin Claude, In PLoS ONE, 2007
... Antibodies to total and phosphorylated STAT3 were from Cell Signaling Technology (Danvers, MA, USA) ...
The putative tumor suppressor gene PTPN13/PTPL1 induces apoptosis through insulin receptor substrate-1 dephosphorylation
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Chang Zhijie et al., In Breast Cancer Research : BCR, 2006
... Anti-Myc (9E10), anti-HA (F-7), anti-GFP (FL), anti-pSTAT3(Tyr705, B-7), anti-pSTAT3(Ser727), anti-STAT3 (F-2) and anti-STAT3 (C-20) antibodies, and protein G/A plus agarose beads were purchased from Santa Cruz Biotechnology, and anti-β-Actin (AC-15) antibody ...
Inhibition of interleukin-1beta-induced group IIA secretory phospholipase A2 expression by peroxisome proliferator-activated receptors (PPARs) in rat vascular smooth muscle cells: cooperation between PPARbeta and the proto-oncogene BCL-6
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DeCoster Mark A et al., In Journal of Neuroinflammation, 2006
... The antibodies against β-actin, phospho-JAK3, phospho-STAT3, JAK3, and STAT3 were purchased from Cell Signaling Technology Inc ...
The expression of the von Hippel-Lindau gene product and its impact on invasiveness of human breast cancer cells
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Rozakis-Adcock M et al., In Oncogene, 2006
... Antibodies to phospho-Met Y1234/5, phospho-Akt Ser 473, Akt, phospho-STAT3, STAT3, phosphotyrosine residues (4G10) and HRP-linked secondary antibodies were purchased from Cell Signaling Technologies (Beverly, MA, USA) ...
STAT3 polymorphism predicts interferon-alfa response in patients with metastatic renal cell carcinoma
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Bauer Joseph Alan, In PLoS ONE, 2006
... For fluorescence polarization assays, STAT3 protein (>90% purity) was purchased from Abcam (Cambridge, MA) ...
The effect of bovine serum albumin on the membrane potential and reactive oxygen species generation in succinate-supported isolated brain mitochondria.
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Combs Colin, In PLoS ONE, 2006
... Primary antibody to GFAP was from Dako (rabbit, Carpenteria, CA), to tyrosine705-phosphorylated STAT3 (pSTAT3) was from Cell Signaling Technology, Inc ...
Raf kinase inhibitory protein knockout mice: expression in the brain and olfaction deficit.
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Yamaoka Yoshio, In PLoS ONE, 2006
... The antibodies to STAT3 pS727 and pY705 and PARP were purchased from Cell Signaling Technology (Beverly, MA) and ...
The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1
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Benistant Christine et al., In The Journal of Cell Biology, 2005
... Stat3 from Cell Signaling Technology, anti-Stat3 from Millipore, ...
Inhibition of interleukin-6 with CNTO328, an anti-interleukin-6 monoclonal antibody, inhibits conversion of androgen-dependent prostate cancer to an androgen-independent phenotype in orchiectomized mice
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Bromberg Jacqueline et al., In Breast Cancer Research : BCR, 2005
... ] by using a radiolabeled high-affinity m67 DNA binding probe and an anti-Stat3 antibody for supershifting (Stat3 (K-15): sc-483 X, Santa Cruz Biotechnology, Santa Cruz, CA, USA) ...
Assessing implicit models for nonpolar mean solvation forces: the importance of dispersion and volume terms.
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Schneider-Stock Regine, In PLoS ONE, 2005
... 14-3-3σ, 14-3-3θ, 14-3-3β, GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA), 14-3-3γ, 14-3-3η, Stat3, pY705-Stat3, pS727-Stat3 antibodies (Cell Signaling, Danvers, MA), HA tag, ...
Targeting signal-transducer-and-activator-of-transcription-3 for prevention and therapy of cancer: modern target but ancient solution
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Claret Francois X et al., In Breast Cancer Research : BCR, 2005
... The following antibodies were used in the study: C/EBP-α (N-19), C/EBP-β (C-19), GATA-1 (H-200), and Stat3 (C-20) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) ...
The signal transduction networks required for phosphorylation of STAT1 at Ser727 in mouse epidermal JB6 cells in the UVB response and inhibitory mechanisms of tea polyphenols
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Verma Ajit K. et al., In Oncogene, 2004
... pStat3Tyr705, Bcl-xL, COX-2, β-actin, donkey anti-goat immunoglobulin (IgG)-FITC for PKCε and donkey anti-rabbit IgG-rhodamine for Stat3 (Santa Cruz Biotechnology, Santa Cruz, CA); and ...
Epigenetic changes to the MDR1 locus in response to chemotherapeutic drugs.
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Virgin Skip W., In PLoS Biology, 2004
... Antibodies for the following targets were used: STAT1 (BD Biosciences Franklin Lakes, NJ, USA), STAT3 (Santa Cruz Biotech, Santa Cruz, CA, USA), HA (Cell Signaling Technology, Beverly, MA, USA), ...
The JAK3 inhibitor CP-690550 selectively reduces NK and CD8+ cell numbers in cynomolgus monkey blood following chronic oral dosing.
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Bunting Kevin D., In PLoS ONE, 2003
... Phospho-STAT3 (Tyr705) D3A7 rabbit monoclonal antibody, Cell Signaling Technology (CST) #9145 ...
Transformation fingerprint: induced STAT3-C, v-Src and Ha-Ras cause small initial changes but similar established profiles in mRNA.
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Zimmer Jacques, In PLoS ONE, 2003
TLR4 (clone UT41), CD25 Abs and Stat3 inhibitory peptide (Stat3I) were from eBioscience (San Diego, CA) ...
Mitochondrial survivin inhibits apoptosis and promotes tumorigenesis.
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Schneider Guenter, In PLoS ONE, 2003
... phospho (p)-EGFR (Y1068, Y1045), Bcl-xL, Mcl-1, Bid, p-Bad (S112), Bad, PI3K, ERK, p-ERK (T202/Y204), STAT3, p-STAT3 (Y705), aldolase, and H3 (Cell Signaling, Danvers, MA, USA) ...
Anti-invasive gene expression profile of curcumin in lung adenocarcinoma based on a high throughput microarray analysis.
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Singh Rana Pratap, In PLoS ONE, 2003
... -pSTAT3 and STAT3 were purchased from Cell Signaling Technology (Beverly,MA) ...
Thrombin Up-regulates Tissue Factor Pathway Inhibitor-2 Synthesis through a Cyclooxygenase-2-dependent, Epidermal Growth Factor Receptor-independent Mechanism
Supplier
Rosenbaum Jean et al., In Comparative Hepatology, 2003
... mAb B-R3 was from Diaclone (Besançon, France), anti-gp80 mAb M91 was from Coulter-Immunotech (Marseille, France), anti-phospho-STAT-3 (Tyr705) was from Cell Signaling Technology (Beverly, MA) ...
Apoptosis pathway in cancer and cancer therapy
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Fahmy Hesham et al., In Marine Drugs, 2003
... Mouse monoclonal antibodies against β-actin, Cdk4 and STAT-3, rabbit antibodies against cycline D1 and D2, goat anti-mouse and goat anti-rabbit both horseradish-conjugated (HRP) secondary antibodies were purchased from Santa Cruz Biotechnology, Inc ...
Matrix metalloproteinase activity and immunohistochemical profile of matrix metalloproteinase-2 and -9 and tissue inhibitor of metalloproteinase-1 during human dermal wound healing
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He Shaoheng et al., In BMC Cell Biology, 2003
... AG490, LY294002, phospho-p44/42 MAPK antibody, unphospho-p44/42 MAPK antibody, phospho-Akt antibody, unphospho-Akt antibody, phospho-STAT3 antibody and unphospho-STAT3 antibody were purchased from Cell Signaling Technology (Beverly, MA, USA) ...
The tyrosine kinase Tyk2 controls IFNAR1 cell surface expression.
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Buday Laszlo, In PLoS ONE, 2002
... : Antibodies against pStat3, Stat3 (Cell Signaling), against IL-10R1 (polyclonal, C20 ...
Receptor trafficking controls weak signal delivery: a strategy used by c-Met for STAT3 nuclear accumulation
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Parker Peter J. et al., In The Journal of Cell Biology, 2001
... were the following: anti–phospho–c-Met (Tyrosine 1349 or Tyrosine 1234/1235), pan-ERK, phospho-p42/p44 ERK, mouse monoclonal anti–pan-STAT3, phospho-STAT3 (Y706; S727), PKCα (BD Biosciences), anti–pan-STAT3, and chc (Santa ...
BMPs signal alternately through a SMAD or FRAP–STAT pathway to regulate fate choice in CNS stem cells
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McKay Ronald D.G. et al., In The Journal of Cell Biology, 2001
... DakoCytomation; monoclonal anti-SMA and anti-FLAG from Sigma-Aldrich; polyclonal anti-HA from BABCO; monoclonal anti-FKBP12, anti-FRAP, and anti-Stat3 from Transduction Laboratories; polyclonal anti-phosphoStat3 (Tyr705 and Ser727) from Santa Cruz Biotechnology, Inc.; monoclonal anti–BMPR-IA from ...
Processing of gene expression data generated by quantitative real-time RT-PCR.
Supplier
Harhaj Edward, In PLoS ONE, 2001
... Anti-phospho-Stat1 (Tyr701) and anti-phospho-Stat3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA, USA) ...
SOCS proteins: negative regulators of cytokine signaling.
Supplier
Hartl Dominik, In PLoS ONE, 2000
... 9205), phospho-STAT3 (Tyr705) (Cell Signaling, Cat ...
A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube
Supplier
Farrar William L et al., In Molecular Cancer, 1999
... LFM-A13 (Tocris, Ellisville, MO), MEK inhibitor PD98059 (Gibco, Carlsbad, CA), JAK inhibitor AG490 (Gibco), and STAT3 inhibitor Stattic (Sigma Aldrich, St ...
Generalized lacZ expression with the ROSA26 Cre reporter strain.
Supplier
O'Rahilly Stephen, In PLoS Biology, 1998
... was purchased from Santa Cruz, anti-JAK2 antibody from Invitrogen, anti-phosphotyrosine antibody from Upstate, anti-P-Stat3 and anti-Stat3 antibodies from Cell Signaling, and anti-actin antibody from ...
Rat embryo fibroblasts transformed by c-Jun display highly metastatic and angiogenic activities in vivo and deregulate gene expression of both angiogenic and antiangiogenic factors
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Gibson Spencer B et al., In BMC Cancer, 1998
... and total Erk1/2, XIAP and STAT3 antibodies, as well as siRNA for c-Jun (#6203) and Stat3 siRNA I (#6580) and control siRNA (#6201 fluorescein conjugate) were purchased from Cell Signaling Technology, VEGF antibody was ...
Signal transduction and transcription factors in rheumatic disease
Supplier
Peng Daizhi et al., In Evidence-based Complementary and Alternative Medicine : eCAM, 1998
... Rabbit anti-phospho-Jak2 (Tyr1007/1008), anti-STAT3 polyclonal antibodies were purchased from Biovision (CA, USA) ...
STAT3 mediates the survival signal in oncogenic ras-transfected intestinal epithelial cells
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Asano T et al., In British Journal of Cancer, 1997
... The antibodies used in this study were antibodies against phospho-STAT3 (p-STAT3, Cell Signaling Technology Inc., Beverly, MA, ...
Signal transduction via platelet-derived growth factor receptors.
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Samuel Jane-Lise, In PLoS ONE, 1997
... kinase 1/2 (ERK1/2), Akt, glycogen synthase kinase-3β (GSK-3β) and signal transducer and activator of transcription 3 (STAT3) were obtained from Cell Signaling technology (Danvers, MA) ...
Functional Inactivation of CXC Chemokine Receptor 4–mediated Responses through SOCS3 Up-regulation
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Mellado Mario et al., In The Journal of Experimental Medicine, 1993
... For immunostaining, we used anti-STAT3 and -phospho-STAT3 antibody (New England Biolabs, Inc.) ...
Anaesthesia techniques for midazolam and flumazenil-an overview
Supplier
Iida Hiroki et al., In Journal of Neuroinflammation, 1989
... SAPK/JNK, phospho-specific inhibitory kappa B (IκB), IκB, phospho-specific signal transducer and activator of transcription (STAT)3 and STAT3 antibodies were purchased from Cell Signaling (Beverly, MA) ...
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