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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

Synaptosomal-associated protein, 25kDa

SNAP-25, Synaptosomal-Associated Protein 25, SUP
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: CAN, Synaptobrevin, V1a, HAD, VAMP2
Papers using SNAP-25 antibodies
Class II Phosphoinositide 3-Kinase Regulates Exocytosis of Insulin Granules in Pancreatic β Cells*
Maffucci Tania et al., In The Journal of Biological Chemistry, 2007
... anti-p110β (1:500), anti-phospho-Thr-308Akt (1:500), anti-Akt (1:1000), anti-ERK2 (1:1000), anti-IRS2 (1:500), anti-syntaxin 1 (1:1000), anti-syntaxin 4 (1:500), and anti-SNAP25 (1:1000) were from Santa Cruz Biotechnology (Santa Cruz, CA), anti-phosphotyrosine ...
Cortactin in tumor invasiveness
Hotchin Neil A., In PLoS ONE, 2007
... 6) were transfected with 5 micrograms of plasmid DNA using SuperFect (Qiagen).
Structure of the 70S ribosome complexed with mRNA and tRNA
Wang En-Duo et al., In Nucleic Acids Research, 2005
... 2+–NTA Superflow was purchased from Qiagen, Inc ...
Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor βlll spectrin
Neefjes Jacques et al., In The Journal of Cell Biology, 2004
... Glued fusion proteins, the same fragments were cloned into the EcoRI and BamHI sites of vector pMAL-c2X (New England Biolabs).
Roles for gliotransmission in the nervous system
Meldolesi Jacopo et al., In The Journal of Cell Biology, 2004
... and anti-VAMP2 mAb’s and anti-Munc18, anti-Syt4, and anti-SNAP23 rabbit pAb’s were obtained from Synaptic Systems; anti-SNAP25 mAb was obtained from Covance; anti-S100β rabbit pAb was purchased from Dako; anti-NPY sheep pAb, anti-GFAP, ...
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Papers on SNAP-25
Structure, Mechanism and Therapeutic Utility of Immunosuppressive Oligonucleotides.
Klinman et al., Frederick, United States. In Pharmacol Res, Feb 2016
This work reviews the types of immunosuppressive oligodeoxynucleotide (Sup ODN) and compares their therapeutic activity against diseases characterized by pathologic levels of immune stimulation ranging from autoimmunity to septic shock to cancer (see graphical abstract).
Role of P-glycoprotein in mediating rivastigmine effect on amyloid-β brain load and related pathology in Alzheimer's disease mouse model.
Kaddoumi et al., Monroe, United States. In Biochim Biophys Acta, Feb 2016
Moreover, rivastigmine demonstrated a P-gp expression dependent neuroprotective effect that was highest in APP(+)/mdr1(+/+)>APP(+)/mdr1(+/-)>APP(+)/mdr1(-/-) as determined by expression of synaptic markers PSD-95 and SNAP-25 using Western blot analysis.
SNARE-mediated cholesterol movement to mitochondria supports steroidogenesis in rodent cells.
Kraemer et al., Palo Alto, United States. In Mol Endocrinol, Feb 2016
Our results from reconstitution experiments along with knockdown studies in rat primary granulosa cells and in a Leydig cell line show that NSFα, SNAP25, syntaxin-5 and syntaxin-17 facilitate the transport of cholesterol to mitochondria.
Caffeine exposure during rat brain development causes memory impairment in a sex selective manner that is offset by caffeine consumption throughout life.
Porciúncula et al., Porto Alegre, Brazil. In Behav Brain Res, Feb 2016
Both caffeine treatment regimens decreased GFAP (as an astrocyte marker) and SNAP-25 (as a nerve terminals marker) in the hippocampus from male rats.
Quantitative proteomics analysis to identify diffuse axonal injury biomarkers in rats using iTRAQ coupled LC-MS/MS.
Li et al., Chongqing, China. In J Proteomics, Jan 2016
Of the ten candidate DAI biomarkers, four proteins, citrate synthase (CS), synaptosomal-associated protein 25 (Snap25), microtubule-associated protein 1B (MAP1B) and Rho-associated protein kinase 2 (Rock2), were validated by subsequent Western blot and immunohistochemistry analyses.
Relationship of cell-free urine MicroRNA with lupus nephritis in children.
Brunner et al., Cincinnati, United States. In Pediatr Rheumatol Online J, Dec 2015
METHODS: miRNAs (125a, 127, 146a, 150 and 155) were measured using real-time polymerase chain reaction in the urine pellet (PEL) and supernatant (SUP) in 14 patients with active LN, 10 patients with active extra-renal lupus, and 10 controls.
Botulinum neurotoxin type A: Actions beyond SNAP-25?
Lacković et al., Zagreb, Croatia. In Toxicology, Oct 2015
It prevents synaptic vesicle neuroexocytosis by proteolytic cleavage of synaptosomal-associated protein of 25 kDa (SNAP-25).
Impacts of thyroxine combined with donepezil on hippocampal ultrastructures and expressions of synaptotagmin-1 and SNAP-25 in adult rats with hypothyroidism.
Zhu et al., Hefei, China. In Int J Clin Exp Med, 2014
The study aims to observe the impacts of thyroxine (T4) combined with donepezil (DON) on hippocampal ultrastructures and expressions of synaptotagmin-1 and SNAP-25 in adult rats with hypothyroidism.
Risks and benefits of stress ulcer prophylaxis in adult neurocritical care patients: a systematic review and meta-analysis of randomized controlled trials.
Siddiqi et al., Colton, United States. In Crit Care, 2014
The aim of this meta-analysis was to evaluate the risks and benefits of stress ulcer prophylaxis (SUP) in this patient group.
The Synaptic Vesicle Release Machinery.
Xu et al., Dallas, United States. In Annu Rev Biophys, 2014
The SNAREs Syntaxin-1, Synaptobrevin, and SNAP-25 play a central role in membrane fusion, forming SNARE complexes that bridge the vesicle and plasma membranes and that are disassembled by NSF-SNAPs.
Structural basis for recognition of synaptic vesicle protein 2C by botulinum neurotoxin A.
Kammerer et al., China. In Nature, 2014
BoNT/A application causes long-lasting flaccid paralysis of muscles through inhibiting the release of the neurotransmitter acetylcholine by cleaving synaptosomal-associated protein 25 (SNAP-25) within presynaptic nerve terminals.
Reconstitution of the vital functions of Munc18 and Munc13 in neurotransmitter release.
Rizo et al., Wuhan, China. In Science, 2013
Neurotransmitter release depends critically on Munc18-1, Munc13, the Ca(2+) sensor synaptotagmin-1, and the soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptors (SNAREs) syntaxin-1, synaptobrevin, and SNAP-25.
Distinct initial SNARE configurations underlying the diversity of exocytosis.
Tokumaru et al., Tokyo, Japan. In Physiol Rev, 2012
The initial SNARE configurations depend on the particular SNARE subtype (syntaxin, SNAP25, or VAMP), priming proteins (Munc18, Munc13, CAPS, complexin, or snapin), triggering proteins (synaptotagmins, Doc2, and various protein kinases), and the submembraneous cytomatrix, and they are the key to determining the kinetics of subsequent exocytosis.
Accumulation of SNAP25 in mouse gustatory and somatosensory cortices in response to food and chemical stimulation.
Misaka et al., Yokohama, Japan. In Neuroscience, 2012
recently ingested chemical signals in the oral cavity during weaning increase the accumulation of SNAP25 in the gustatory and somatosensory cortices and promote neural plasticity during the development of the gustatory and somatosensory nervous systems.
Identification of CSPα clients reveals a role in dynamin 1 regulation.
Chandra et al., New Haven, United States. In Neuron, 2012
Using unbiased, systematic proteomics to identify cysteine string protein alpha (CSPalpha) client proteins, study shows that SNAP-25 and the endocytic GTPase dynamin 1 are key clients of the Hsc70-CSPalpha chaperone complex.
Reduced thyroid hormones with increased hippocampal SNAP-25 and Munc18-1 might involve cognitive impairment during aging.
Chen et al., Hefei, China. In Behav Brain Res, 2012
The results of this study suggested that increased hippocampal SNAP-25 and Munc18-1 which seemingly result from decreased serum THs might involve the age-related impairment of spatial learning and memory.
Phospholipase C-related but catalytically inactive protein (PRIP) modulates synaptosomal-associated protein 25 (SNAP-25) phosphorylation and exocytosis.
Hirata et al., Fukuoka, Japan. In J Biol Chem, 2012
PRIP is involved in the regulation of the phospho-states of SNAP-25 by modulating the activity of PP1, thus regulating exocytosis.
Derlin-1 deficiency is embryonic lethal, Derlin-3 deficiency appears normal, and Herp deficiency is intolerant to glucose load and ischemia in mice.
Kokame et al., Suita, Japan. In Plos One, 2011
Derlin-1 deficiency is embryonic lethal, Derlin-3 deficiency appears normal, and Herp deficiency is intolerant to glucose load and ischemia in mice
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Südhof et al., Palo Alto, United States. In Nat Cell Biol, 2011
Data show that deletion of CSPalpha produces an abnormal SNAP-25 conformer that inhibits SNARE-complex formation, and is subject to ubiquitylation and proteasomal degradation.
Fast vesicle fusion in living cells requires at least three SNARE complexes.
Sørensen et al., Göttingen, Germany. In Science, 2010
Simultaneous expression of wild-type SNAP-25 and a mutant unable to support exocytosis progressively altered fusion kinetics and fusion-pore opening, indicating that both proteins assemble into heteromeric fusion complexes.
More papers using SNAP-25 antibodies
Neuronal Death Induced by Nanomolar Amyloid β Is Mediated by Primary Phagocytosis of Neurons by Microglia*
Brown Guy C. et al., In The Journal of Biological Chemistry, 2002
... Dako, β-tubulin III antibody was from Sigma, synapsin I antibody was from Millipore, synaptosomal-associated protein 25 (SNAP-25) (SMI 81) antibody was from Covance, phosphatidylserine antibody was from Abcam, and mouse control IgG ...
Identification of a small, very acidic constitutive nucleolar protein (NO29) as a member of the nucleoplasmin family
Altman Sidney et al., In The Journal of Cell Biology, 1996
... 5 cells grown in 60 × 15 mm style petri dishes containing glass coverslips were transfected with plasmid DNA (2–5 μg) using the SuperFect reagent (Qiagen) following the manufacturer's instructions ...
Targeted interleukin-2 therapy for spontaneous neuroblastoma metastases to bone marrow
Lesniak Maciej S., In PLoS ONE, 1996
... 2 flask in Mesencult basal medium supplemented with Mesenchymal Stem Cell Stimulatory Supplement (StemCell Technologies, Vancouver, BC, Canada) ...
Folding, Quality Control, and Secretion of Pancreatic Ribonuclease in Live Cells*
Helenius Ari et al., In The Journal of Biological Chemistry, 1952
... 35S]methionine/cysteine were from GE Healthcare, and Superfect® Transfection Reagent was from Qiagen.
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