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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

Lymphocyte cytosolic protein 2

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: LAT, Src, ZAP-70, Syk, NBS1
Papers using SLP-76 antibodies
Physical and functional association of c-Src and adhesion and degranulation-promoting adaptor protein (ADAP) inosteoclastogenesis in vitro.
Rénia Laurent, In PLoS ONE, 2004
... Nck (recognizing Nck1 and Nck2, Becton Dickinson GmbH, Heidelberg, Germany, #610099), ADAP (BD, #610945), and SLP-76 (Santa Cruz Biotechnology Inc., Santa Cruz, USA, ...
Fc∈RI-mediated recruitment of p53/56lyn to detergent-resistant membrane domains accompanies cellular signaling
Rivera Juan et al., In The Journal of Experimental Medicine, 1994
... Mouse mAbs to Vav, Rac1, LAT, phosphotyrosine (4G10), Grb2, SLP-76, and GFP were purchased from Upstate Biotechnology, Transduction Laboratories, and Clontech.
Papers on SLP-76
Platelet immunoreceptor tyrosine-based activation motif (ITAM) and hemITAM signaling and vascular integrity in inflammation and development.
Bergmeier et al., Chapel Hill, United States. In J Thromb Haemost, Feb 2016
These receptors share common downstream effectors including Syk, SLP-76 and PLCγ2.
Imbalanced signal transduction in regulatory T cells expressing the transcription factor FoxP3.
Benoist et al., Boston, United States. In Proc Natl Acad Sci U S A, Jan 2016
Seeking a comprehensive view, we found that Treg cells have a broadly dampened activation of several pathways and signaling nodes upon TCR-mediated activation, with low phosphorylation of CD3ζ, SLP76, Erk1/2, AKT, or S6 and lower calcium flux.
Novel Molecular Insights into Classical and Alternative Activation States of Microglia as Revealed by Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based Proteomics.
Stevens et al., Tampa, United States. In Mol Cell Proteomics, Dec 2015
The M2c state appears to center around the down-regulation of a key member in the formation of actin-rich phagosomes, SLP-76.
The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells.
Rudd et al., Cambridge, United Kingdom. In Mol Cell, Oct 2015
Here, we show that the immune cell adaptor SLP-76 binds directly to SUMO-RanGAP1 of cytoplasmic fibrils of the NPC, and that this interaction is needed for optimal NFATc1 and NF-κB p65 nuclear entry in T cells.
Evidence for inducible recruitment of Wiskott-Aldrich syndrome protein to T cell receptor-CD3 complex in Jurkat T cells.
Pongcharoen et al., Phitsanulok, Thailand. In Asian Pac J Allergy Immunol, Sep 2015
METHODS: Jurkat T cells were stimulated with anti-TCR antibody and then the cell lysates were immunoprecipitated with anti-CD3 antibody before immunoblotting with antibodies specific to WASp, Nck1, Nck2, SLP-76 and CD3ε molecules.
6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells.
Yen et al., Ithaca, United States. In Plos One, 2014
Moreover, FICZ augments the expression of a number of the members of the RA-induced signalsome, such as c-Cbl, Vav1, Slp76, PI3K, and the Src family kinases Fgr and Lyn.
Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub.
Malissen et al., Marseille, France. In Nat Immunol, 2014
Here we used quantitative mass spectrometry and activated primary CD4(+) T cells from mice in which a tag for affinity purification was knocked into several genes to determine the composition and dynamics of multiprotein complexes that formed around the kinase Zap70 and the adaptors Lat and SLP-76.
HIV-1 Nef limits communication between linker of activated T cells and SLP-76 to reduce formation of SLP-76-signaling microclusters following TCR stimulation.
Fackler et al., Heidelberg, Germany. In J Immunol, 2012
Nef employs a dual mechanism to disturb early TCR signaling by limiting the communication between LAT and SLP-76
Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET.
Barda-Saad et al., Ramat Gan, Israel. In Sci Signal, 2012
both T cell activation and the association between SLP-76 and Nck. After T cell receptor stimulation, SLP-76 was phosphorylated, which enabled the binding of Nck.
Integrin signalling and function in immune cells.
Wang et al., Shanghai, China. In Immunology, 2012
This review discusses the key signalling complexes regulating integrin activation and function in both 'inside-out' and 'outside-in' pathways in T lymphocytes, including kinases, SLP-76, VAV1, ADAP, SKAP-55, RapL, RIAM, Rap1, Talin and Kindlin.
The adaptor protein SLP-76 regulates HIV-1 release and cell-to-cell transmission in T cells.
Ganju et al., Columbus, United States. In J Immunol, 2012
our studies demonstrate a novel role for the adaptor molecule SLP-76 in regulating HIV-1 infection in T cells
Crucial role of SLP-76 and ADAP for neutrophil recruitment in mouse kidney ischemia-reperfusion injury.
Zarbock et al., Münster, Germany. In J Exp Med, 2012
SLP-76 and ADAP are involved in E-selectin-mediated integrin activation and neutrophil recruitment to inflamed kidneys, which may underlie the development of life-threatening ischemia-reperfusion-induced acute kidney injury in humans.
Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells.
Long et al., Rockville, United States. In Sci Signal, 2011
Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells.
The osteoclast and its unique cytoskeleton.
Teitelbaum, Saint Louis, United States. In Ann N Y Acad Sci, 2011
When occupied, α(v) β(3) activates a canonical signaling complex consisting of c-Src, Syk, Dap12, Slp76, Vav 3, and Rac that permits the cell to spread and form actin rings.
Functional nanoscale organization of signaling molecules downstream of the T cell antigen receptor.
Samelson et al., Bethesda, United States. In Immunity, 2011
Surprisingly, the adaptor SLP-76 localized to the periphery of LAT clusters.
The kinase GLK controls autoimmunity and NF-κB signaling by activating the kinase PKC-θ in T cells.
Tan et al., Taiwan. In Nat Immunol, 2011
TCR signaling activated GLK by inducing its direct interaction with the upstream adaptor SLP-76.
Platelets: covert regulators of lymphatic development.
Kahn et al., Philadelphia, United States. In Arterioscler Thromb Vasc Biol, 2010
Genetic experiments demonstrate that podoplanin, a transmembrane protein expressed on lymphatic endothelial cells, engages the platelet C-type lectin-like receptor 2 (CLEC-2) when exposed to blood, leading to SYK-SLP-76-dependent platelet activation.
T cell activation at the immunological synapse: vesicles emerge for LATer signaling.
Billadeau, Rochester, United States. In Sci Signal, 2009
Src homology 2 (SH2) domain-containing leukocyte phosphoprotein of 76 kD (SLP-76), which is cytosolic, and LAT, which is membrane-associated, are key adaptor proteins phosphorylated by TCR-associated ZAP-70, and they form microclusters at the IS.
T cell costimulation via the integrin VLA-4 inhibits the actin-dependent centralization of signaling microclusters containing the adaptor SLP-76.
Bunnell et al., Boston, United States. In Immunity, 2008
study reports that T cell costimulation by the integrin VLA-4 (alpha4beta1) required SLP-76 domains implicated in microcluster assembly
Complementation in trans of altered thymocyte development in mice expressing mutant forms of the adaptor molecule SLP76.
Koretzky et al., Philadelphia, United States. In Immunity, 2008
N-terminal tyrosines of SLP76 are required for thymocyte selection but can function on separate molecules to support TCR signaling
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