Development and functional characterization of extrahepatic cholangiocyte lines from normal rats.
Temple, United States. In Dig Liver Dis, Nov 2015
At ∼85% confluence, in extrahepatic cholangiocytes we measured: (i) cell size and distribution, and expression for cytokeratin-19, secretin, secretin receptor and somatostatin receptor type II (SSTR2), cystic fibrosis transmembrane conductance regulator (CFTR), chloride bicarbonate anion exchanger 2 (AE2), vascular endothelial growth factor-A (VEGF-A) and nerve growth factor (NGF); and (ii) the effect of secretin and/or somatostatin on 3'-5'-cyclic adenosine monophosphate (cAMP) levels and proliferation.
Directed differentiation of cholangiocytes from human pluripotent stem cells.
Toronto, Canada. In Nat Biotechnol, Aug 2015
Using three-dimensional culture, the protocol yields cystic and/or ductal structures that express mature biliary markers, including apical sodium-dependent bile acid transporter, secretin receptor, cilia and cystic fibrosis transmembrane conductance regulator (CFTR).
Cerebellar secretin modulates eyeblink classical conditioning.
Burlington, United States. In Learn Mem, 2014
In Experiment 1, rats received intracerebellar infusions of the secretin receptor antagonist 5-27 secretin or vehicle into the lobulus simplex of cerebellar cortex immediately prior to sessions 1-3 of acquisition.
Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells.
Villejuif, France. In Hepatology, 2014
hESC- and HepaRG-derived cholangiocyte-like cells expressed markers of cholangiocytes including cytokeratin 7 and osteopontin, and the transcription factors SOX9 and hepatocyte nuclear factor 6. The cells also displayed specific proteins important for cholangiocyte functions including cystic fibrosis transmembrane conductance regulator, secretin receptor, and nuclear receptors.
Prolonged administration of secretin to normal rats increases biliary proliferation and secretin-induced ductal secretory activity.
Temple, United States. In Hepatobiliary Surg Nutr, 2014
Enhanced biliary proliferation [for example after bile duct ligation (BDL) and partial hepatectomy] is associated with increased expression of secretin receptor (SR), cystic fibrosis transmembrane conductance regulator (CFTR) and Cl(-)/HCO3 (-) anion exchanger 2 and secretin-stimulated ductal secretion, whereas loss/damage of bile ducts [for example after acute carbon tetrachloride (CCl4) administration] is associated with reduced secretin-stimulated ductal secretory activity.
The central mechanisms of secretin in regulating multiple behaviors.
Hong Kong, Hong Kong. In Front Endocrinol (lausanne), 2013
SCT and its receptor (SCTR) are widely expressed in different brain regions, where they exert multiple cellular functions including neurotransmission, gene expression regulation, neurogenesis, and neural protection.