GoPubMed Proteins lists recent and important papers and reviews for
proteins. Page last changed on 14 Mar 2013.
RPO21 Rpo21p
Rpb1, RNA polymerase II subunit, RPO21, POLR2A
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008] (from
NCBI)
Sponsored links
Papers using
Rpb1
antibodies
p38 mitogen-activated protein kinase pathway promotes skeletal muscle differentiation. Participation of the Mef2c transcription factor
Supplier
In The EMBO Journal, 1998
... antibody (Millipore 06-755), Myog (Santa Cruz SC-576), Suz12 (Abcam ab12073), Mef2 (Santa Cruz sc-17785, sc-13917), RPB1 (Abcam ab5408), and Ezh2 (Zymed ...
Papers on
Rpb1
Biogenesis of RNA Polymerases II and III Requires the Conserved GPN Small GTPases in Saccharomyces cerevisiae.
New
Vancouver, Canada. In Genetics, 31 Mar 2013
In this study, we show that the nuclear import defect of iwr1Δ, but not the GPN2 or GPN3 mutant defects, is partially suppressed by fusion of a nuclear localization signal to the RNA polymerase II subunit Rpb3.
Genome-assisted development of nuclear intergenic sequence markers for entomopathogenic fungi of the Metarhizium anisopliae species complex.
New
Beltsville, United States. In Mol Ecol Resour, 31 Mar 2013
This prior work was conducted with commonly used markers in fungal phylogenetics (BTUB, RPB1, RPB2 and TEF), which likely under-represent diversity in the M. anisopliae complex.
Magnaporthiopsis, a new genus in Magnaporthaceae (Ascomycota).
New
New Brunswick, United States. In Mycologia, 28 Mar 2013
The phylogenetic relationships among taxa in the Magnaporthaceae are investigated based on DNA sequences of multiple genes including SSU, ITS, LSU, MCM7, RPB1 and TEF1.
A phylogeny of the highly diverse cup-fungus family Pyronemataceae (Pezizomycetes, Ascomycota) clarifies relationships and evolution of selected life history traits.
New
Cambridge, United States. In Mol Phylogenet Evol, 08 Mar 2013
Three hundred ninety four new sequences were generated during this project, with the following numbers for each gene: RPB1 (124), RPB2 (99), EF-1α (120) and LSU rDNA (51).
Antitumor activity of a pyrrole-imidazole polyamide.
New
Pasadena, United States. In Proc Natl Acad Sci U S A, 01 Mar 2013
Polyamide treatment results in a time- and dose-dependent depletion of the RNAP2 large subunit RPB1 that is preventable with proteasome inhibition.
The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.
New
Frederick, United States. In J Biol Chem, 25 Feb 2013
Alleles of RPB1 (RPO21) with elevated slippage rates were identified among 6-azauracil-sensitive mutants and were also isolated using a slippage-dependent reporter gene.
Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria.
New
Peoria, United States. In Fungal Genet Biol, 25 Feb 2013
We conducted maximum likelihood (ML), maximum parsimony (MP) and Bayesian (B) analyses on partial DNA-directed RNA polymerase II largest (RPB1) and second largest subunit (RPB2) nucleotide sequences of 93 fusaria to infer the first comprehensive and well-supported phylogenetic hypothesis of evolutionary relationships within the genus and 20 of its near relatives.
Sequence variation in two protein-coding genes correlates with Mycelial Compatibility Groupings in Sclerotium rolfsii.
New
Córdoba, Spain. In Phytopathology, 09 Feb 2013
These included restriction fragment length polymorphism patterns of the Internal Transcribed Spacer region of nuclear rDNA (ITS-RFLP) and sequence analysis of two protein-coding genes: Translation Elongation Factor-1 alpha (EF1α) and RNA polymerase II subunit two (RPB2).
RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3' end processing.
Impact
GeneRIF
New York City, United States. In Science, 2011
evidence that phosphorylation of Rpb1 CTD Thr4 residues is required specifically for histone mRNA 3' end processing, functioning to facilitate recruitment of 3' processing factors to histone genes
Parcs/Gpn3 is required for the nuclear accumulation of RNA polymerase II.
GeneRIF
San Luis Potosí, Mexico. In Biochim Biophys Acta, 2011
Parcs/Gpn3 plays a critical role in the nuclear accumulation of RNAP II, and this function explains the relative importance of Parcs/Gpn3 in cell proliferation.
The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing.
GeneRIF
Ramat Gan, Israel. In Plos Biol, 2010
kinetics of RNA polymerase II elongation during co-transcriptional splicing
von Hippel-Lindau-dependent patterns of RNA polymerase II hydroxylation in human renal clear cell carcinomas.
GeneRIF
Cincinnati, United States. In Clin Cancer Res, 2010
Elevated PHD1 concomitant with decreased PHD2 are causatively related to Rpb1 hydroxylation and oncogenesis in human renal clear cell carcinomas with WT VHL gene.
RNA polymerase II binding patterns reveal genomic regions involved in microRNA gene regulation.
GeneRIF
Indianapolis, United States. In Plos One, 2009
Data show that MicroRNA promoter identification based upon RPol II binding patterns provides important temporal and spatial measurements regarding the initiation of transcription.
A phylogenetic overview of the Agaricomycotina.
Review
Worcester, United States. In Mycologia, 2006
Recent phylogenetic analyses by P. Matheny and colleagues combining nuclear rRNA genes with the protein-coding genes rpb1, rpb2 and tef1 support the division of Agaricomycotina into Tremellomycetes, Dacrymycetes and Agaricomycetes.
Evolutionary relationships among basal fungi (Chytridiomycota and Zygomycota): Insights from molecular phylogenetics.
Review
Ibaraki, Japan. In J Gen Appl Microbiol, 2005
Evolutionary relationships of the two basal fungal phyla Chytridiomycota and Zygomycota are reviewed in light of recent molecular phylogenetic investigation based on rDNA (nSSU, nLSU rDNA), entire mitochondrial genomes, and nuclear protein coding gene sequences (e.g., EF-1alpha, RPB1).
CTD phosphatase: role in RNA polymerase II cycling and the regulation of transcript elongation.
Review
In Prog Nucleic Acid Res Mol Biol, 2001
The repetitive C-terminal domain (CTD) of the largest RNA polymerase II subunit plays a critical role in the regulation of gene expression.
Complementary DNA sequencing: expressed sequence tags and human genome project.
Impact
Bethesda, United States. In Science, 1991
Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor.
RNA polymerase II: subunit structure and function.
Review
Cambridge, United States. In Trends Biochem Sci, 1990
Epitope tagging and other experiments made possible by the cloning of these genes have provided a clearer picture of RNA polymerase II subunit composition, stoichiometry and function, and set the stage for further investigating the dialogue between RNA polymerase II and transcription factors.
A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases.
Impact
Cambridge, United States. In Cell, 1989
Two of these suppressors, SIT1 and SIT2, are encoded by RPB1 and RPB2, the genes for the two largest subunits of RNA polymerase II.
Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.
Impact
In Cell, 1985
We have determined the nucleotide sequence of two yeast RNA polymerase genes, RPO21 and RPO31, which encode the largest subunits of RNA polymerases II and III, respectively.
