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Replication protein A2, 32kDa

RPA2, RPA32, RFA2, A135
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] (from NCBI)
Top mentioned proteins: RPA, 14-kDa, CAN, Chk1, POLYMERASE
Papers using RPA2 antibodies
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
Supplier
Kirkpatrick David T., In PLoS ONE, 2004
... The antibodies used were anti-RPA2 (Calbiochem), anti-phospho-RPA2 (S4, S8) (Bethyl Laboratories), anti-phospho-RPA2 (S33) (Bethyl ...
Papers on RPA2
TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism.
New
Impact
Jackson et al., Köln, Germany. In Nat Genet, Jan 2016
We establish that TRAIP relocalizes to sites of DNA damage, where it is required for optimal phosphorylation of H2AX and RPA2 during S-phase in response to ultraviolet (UV) irradiation, as well as fork progression through UV-induced DNA lesions.
Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.
New
Jäättelä et al., Copenhagen, Denmark. In Nucleic Acids Res, Jan 2016
Its depletion compromises the survival of human U2OS osteosarcoma and HeLa cervix carcinoma cells and impairs the DNA damage-induced phosphorylation of replication protein A2 (RPA2) and the recruitment of DNA endonuclease RBBP8/CtIP to DNA double strand breaks.
EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.
New
Hromas et al., Göttingen, Germany. In Plos Genet, Dec 2015
EEPD1 is also required for proper ATR and CHK1 phosphorylation, and formation of gamma-H2AX, RAD51 and phospho-RPA32 foci.
Extensive RPA2 hyperphosphorylation promotes apoptosis in response to DNA replication stress in CHK1 inhibited cells.
New
Meuth et al., Sheffield, United Kingdom. In Nucleic Acids Res, Dec 2015
When CHK1 is inhibited during DNA replication stress, RPA2 is extensively hyperphosphorylated.
Maternal BCAS2 protects genomic integrity in mouse early embryonic development.
New
Li et al., Beijing, China. In Development, Dec 2015
In addition, phosphorylated RPA2 cannot localise to the DNA damage sites in mouse zygotes with disrupted maternal BCAS2.
Cdk5 promotes DNA replication stress checkpoint activation through RPA-32 phosphorylation, and impacts on metastasis free survival in breast cancer patients.
New
Fernet et al., Orsay, France. In Cell Cycle, Nov 2015
Cdk5 depletion was associated with reduced priming phosphorylations of RPA32 serines 29 and 33 and SMC1-Serine 966 phosphorylation, lower levels of RPA serine 4 and 8 phosphorylation and DNA damage measured using the alkaline Comet assay, gamma-H2AX signal intensity, RPA and Rad51 foci, and sister chromatid exchanges resulting in impaired intra-S checkpoint activation and subsequently higher numbers of chromatin bridges.
The role of carrier ligands of platinum(II) anticancer complexes in the protein recognition of Pt-DNA adducts.
New
Guo et al., Nanjing, China. In Chem Commun (camb), Oct 2015
Interestingly, an important component of the replication protein A complex, RPA2, was found to bind to kiteplatin much more tightly than other proteins.
hPso4/hPrp19: a critical component of DNA repair and DNA damage checkpoint complexes.
Review
New
Mahajan, Tampa, United States. In Oncogene, Oct 2015
It not only senses DNA damage, binds double-stranded DNA in a sequence-independent manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, but also regulates RNA splicing and mitotic spindle formation in its integral capacity as a scaffold for a multimeric core complex.
Crystal structure of the 14-subunit RNA polymerase I.
Impact
Müller et al., Madrid, Spain. In Nature, 2013
The A49-A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2.
Discovery of ML367, inhibitor of ATAD5 stabilization
Review
Maloney et al., Bethesda, United States. In Unknown Journal, 2013
Interestingly, ML367 was found to block general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation.
4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2.
GeneRIF
Chang et al., Tainan City, Taiwan. In Febs Lett, 2012
4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2
The UNG2 Arg88Cys variant abrogates RPA-mediated recruitment of UNG2 to single-stranded DNA.
GeneRIF
Slupphaug et al., Trondheim, Norway. In Dna Repair (amst), 2012
Data show that the R88C variant impairs binding of the R88C variant impairs binding of uracil-DNA glycosylase UNG2 to replication protein A RPA2.
Mechanical loading induces the expression of a Pol I regulon at the onset of skeletal muscle hypertrophy.
GeneRIF
Nader et al., Stockholm, Sweden. In Am J Physiol Cell Physiol, 2012
Increased transcription and protein expression of polymerase I regulon is consistent with the increase in ribosomal RNA following skeletal muscle hypertrophy.
Replication protein A: a reliable biologic marker of prognostic and therapeutic value in human astrocytic tumors.
GeneRIF
Korkolopoulou et al., Athens, Greece. In Hum Pathol, 2011
Replication protein A1, replication protein A2, and cyclins D2 and D3 seem to have a parallel role in the promotion of cell cycle in astrocytic tumors being implicated in the malignant progression of these neoplasms.
DNA-PK-dependent RPA2 hyperphosphorylation facilitates DNA repair and suppresses sister chromatid exchange.
GeneRIF
Myung et al., Bethesda, United States. In Plos One, 2010
RPA2 hyperphosphorylation by DNA-PK in response to DNA double-strand breaks blocks unscheduled homologous recombination and delays mitotic entry.
Eukaryotic single-stranded DNA binding proteins: central factors in genome stability.
Review
Nasheuer et al., Galway, Ireland. In Subcell Biochem, 2009
Recently a human homologue to the RPA2 subunit, called RPA4, was discovered and RPA4 can substitute for RPA2 in the RPA complex resulting in an "alternative" RPA (aRPA), which can bind to ssDNA with similar affinity as canonical RPA.
Replication, repair, and reactivation.
Review
Martienssen et al., New York City, United States. In Dev Cell, 2005
In a recent issue of Current Biology, Kapoor et al. (2005) and Elmayan et al. (2005) illuminate the linkage between DNA replication and repair and transcriptional gene silencing in plants by showing that mutants in RPA2, a homolog of yeast and mammalian replication protein A, exhibit loss of silencing at transgene loci as well as some transposable elements.
Molecular pathology of the MEN1 gene.
Review
Marx et al., Bethesda, United States. In Ann N Y Acad Sci, 2004
It may partner with junD, NF-kB, PEM, SMAD3, RPA2, FANCD2, NM23beta, nonmuscle myosin heavy chain II-A, GFAP, and/or vimentin.
Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA.
Impact
Chazin et al., Los Angeles, United States. In Cell, 2000
We have shown that a globular domain at the C terminus of subunit RPA32 contains a specific surface that interacts in a similar manner with the DNA repair enzyme UNG2 and repair factors XPA and RAD52, each of which functions in a different repair pathway.
Evidence for chain molecules enriched in carbon, hydrogen, and oxygen in comet halley.
Impact
Mendis et al., In Science, 1987
In situ measurements of the composition and spatial distribution of heavy thermal positive ions in the coma of comet Halley were made with the heavy-ion analyzer RPA2-PICCA aboard the Giotto spacecraft.
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