Chromosome position determines the success of double-strand break repair.
Waltham, United States. In Proc Natl Acad Sci U S A, Feb 2016
The repair efficiency of a donor locus can be improved by four factors: slower 5' to 3' resection of the DSB ends, increased abundance of replication protein factor A (RPA), longer shared homology, or presence of a recombination enhancer element adjacent to a donor.
HELB Is a Feedback Inhibitor of DNA End Resection.
Toronto, Canada. In Mol Cell, Feb 2016
HELB is recruited to ssDNA by interacting with RPA and uses its 5'-3' ssDNA translocase activity to inhibit EXO1 and BLM-DNA2, the nucleases catalyzing resection.
In vitro chromatin templates to study nucleotide excision repair.
West Lafayette, United States. In Dna Repair (amst), Dec 2015
At least three systems have been used to analyze the effect of nucleosome folding on nucleotide excision repair (NER) in vitro: (a) human cell extracts that have to rely on labeling of repair synthesis to monitor DNA repair, due to very low repair efficacy; (b) Xenopus oocyte nuclear extracts, that have very robust DNA repair efficacy, have been utilized to follow direct removal of DNA damage; (c) six purified human DNA repair factors (RPA, XPA, XPC, TFIIH, XPG, and XPF-ERCC1) that have been used to reconstitute excision repair in vitro.
hPso4/hPrp19: a critical component of DNA repair and DNA damage checkpoint complexes.
Tampa, United States. In Oncogene, Oct 2015
It not only senses DNA damage, binds double-stranded DNA in a sequence-independent manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, but also regulates RNA splicing and mitotic spindle formation in its integral capacity as a scaffold for a multimeric core complex.
Telomere elongation chooses TERRA ALTernatives.
Zürich, Switzerland. In Rna Biol, 2014
TERRA and telomeric hybrids act in concert with a number of other factors, including the RNA endoribonuclease RNaseH1 and the single stranded DNA binding protein RPA.