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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 19 Aug 2016.

Ribonuclease H1

RNase H, Ribonuclease H
Top mentioned proteins: CAN, POLYMERASE, ACID, HAD, V1a
Papers using RNase H antibodies
The simulated microgravity environment maintains key metabolic functions and promotes aggregation of primary porcine hepatocytes.
Supplier
Edgar Alasdair J, In BMC Biochemistry, 2000
... Total RNA was reversed transcribed with AMV RNase H- reverse transcriptase (ThermoScript, Life Technologies, UK) at 50°C using ...
Papers on RNase H
Conformational dynamics of stem II of the U2 snRNA.
New
Hoskins et al., Madison, United States. In Rna, Feb 2016
As noted in previous studies, the Mg(2+) toggling we observe parallels previous observations of U2/U6 and Prp8p RNase H domain Mg(2+)-dependent conformational changes.
Unfolding the HIV-1 reverse transcriptase RNase H domain - how to lose a molecular tug-of-war.
New
London et al., United States. In Nucleic Acids Res, Feb 2016
Since the ribonuclease H (RH) domain contains an occult cleavage site located near its center, cleavage must occur either prior to folding or subsequent to unfolding.
Fast Folding Dynamics of an Intermediate State in RNase H Measured by Single-Molecule FRET.
New
Nienhaus et al., In J Phys Chem B, Feb 2016
UNASSIGNED: We have studied the folding kinetics of the core intermediate (I) state of RNase H by using a combination of single-molecule FRET (smFRET) and hidden Markov model analysis.
Transcript RNA supports precise repair of its own DNA gene.
New
Storici et al., Atlanta, United States. In Rna Biol, Jan 2016
These results highlight an antagonistic role of RNase H in RNA-DNA recombination.
High-speed DNA-based rolling motors powered by RNase H.
New
Impact
Salaita et al., Atlanta, United States. In Nat Nanotechnol, Dec 2015
The motors are made from DNA-coated spherical particles that hybridize to a surface modified with complementary RNA; the motion is achieved through the addition of RNase H, which selectively hydrolyses the hybridized RNA.
Is the role of human RNase H2 restricted to its enzyme activity?
Review
New
Cao et al., Hengyang, China. In Prog Biophys Mol Biol, Dec 2015
UNASSIGNED: In human cells, ribonuclease (RNase) H2 complex is the predominant source of RNase H activities with possible roles in nucleic acid metabolism to preserve genome stability and to prevent immune activation.
A computational chemistry perspective on the current status and future direction of hepatitis B antiviral drug discovery.
Review
New
Kulp et al., United States. In Antiviral Res, Nov 2015
Homology modeling is a computational simulation to predict protein structure and has been used to construct conformers of the viral polymerase (reverse transcriptase domain and RNase H domain) and the HBV X protein.
Mechanisms of DNA Transposition.
Review
New
Dyda et al., In Microbiol Spectr, Apr 2015
Here, we review the mechanisms of the four known types of transposition reactions catalyzed by (1) RNase H-like transposases (also known as DD(E/D) enzymes); (2) HUH single-stranded DNA transposases; (3) serine transposases; and (4) tyrosine transposases.
Tyrosine Recombinase Retrotransposons and Transposons.
Review
New
Butler et al., In Microbiol Spectr, Apr 2015
The three groups form distinct clades on phylogenetic trees based on alignments of reverse transcriptase/ribonuclease H (RT/RH) and YR sequences, and also having some structural distinctions.
Mariner and the ITm Superfamily of Transposons.
Review
New
Chalmers et al., In Microbiol Spectr, Apr 2015
The metal ions are coordinated by three or four acidic amino acid residues located within an RNase H-like structural fold.
Enzyme dynamics from NMR spectroscopy.
Review
New
Impact
Palmer, New York City, United States. In Acc Chem Res, Mar 2015
This Account reviews NMR spin relaxation studies of the enzymes ribonuclease HI from mesophilic (Escherichia coli) and thermophilic (Thermus thermophilus) bacteria, E. coli AlkB, and Saccharomyces cerevisiae triosephosphate isomerase to illustrate the contributions of conformational flexibility and dynamics to diverse steps in enzyme mechanism.
Catalytic metal ions and enzymatic processing of DNA and RNA.
Review
New
Impact
De Vivo et al., Genova, Italy. In Acc Chem Res, Mar 2015
In this Account, we discuss the role of multiscale molecular simulations in further disclosing mechanistic insights of 2M-aided catalysis for two prototypical enzymatic targets for drug discovery, namely, ribonuclease H (RNase H) and type II topoisomerase (topoII).
Ancient Origin of the U2 Small Nuclear RNA Gene-Targeting Non-LTR Retrotransposons Utopia.
Jurka et al., Tokyo, Japan. In Plos One, 2014
Oomycete Utopia families contain an "archaeal" RNase H domain upstream of reverse transcriptase (RT), which likely originated from a plant RNase H gene. Analysis of Utopia from oomycetes indicates that multiple lineages of Utopia have been maintained inside of U2 genes with few copy numbers.
Enzyme-assisted target recycling (EATR) for nucleic acid detection.
Review
Impact
Kolpashchikov et al., Orlando, United States. In Chem Soc Rev, 2014
Nucleases used for EATR include sequence-dependent restriction or nicking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII, AP endonuclease, duplex-specific nuclease, DNase I, or T7 exonuclease.
Comparative analysis of RNA sequencing methods for degraded or low-input samples.
Impact
Levin et al., Cambridge, United States. In Nat Methods, 2013
We found that the RNase H method performed best for chemically fragmented, low-quality RNA, and we confirmed this through analysis of actual degraded samples.
Structural basis of the RNase H1 activity on stereo regular borano phosphonate DNA/RNA hybrids.
GeneRIF
Germann et al., Atlanta, United States. In Biochemistry, 2011
On the basis of its nuclear magnetic resonance (NMR) nucleic acid structure, a boranophosphonate-modified, fully R(P) BH(3) DNA/RNA hybrid is predicted not to be a substrate for RNase H1.
Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD.
GeneRIF
Yang et al., Bethesda, United States. In Embo J, 2008
Characterization of full-length enzymes with defective hybrid binding domain indicates that this domain dramatically enhances both the specific activity and processivity of RNase H1.
Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription.
GeneRIF
Yang et al., Bethesda, United States. In Mol Cell, 2007
Report crystal structures of RNase H1 in complex with RNA/DNA hybrids.
The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis.
GeneRIF
Crooke et al., Carlsbad, United States. In Mol Pharmacol, 2007
method for enhancing the human RNase H1 activity of chimeric antisense oligonucleotides
Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate.
GeneRIF
Crooke et al., Carlsbad, United States. In Mol Pharmacol, 2007
THE role substrate structure plays in directing human RNase H1 activity as well as the design of effective antisense oligodeoxyribonucleotides.
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