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Ribosomal protein S18

ribosomal protein S18, Rps18, KE3, HKE3
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] (from NCBI)
Top mentioned proteins: ACID, GAPDH, CAN, POLYMERASE, Actin
Papers on ribosomal protein S18
Verification of suitable and reliable reference genes for quantitative real-time PCR during adipogenic differentiation in porcine intramuscular stromal-vascular cells.
Yang et al., China. In Animal, Feb 2016
NormFinder and geNorm uncovered ACTB, ALDOA and RPS18 as the most three stable genes.
Reference Gene Selection and Evaluation for Gene Expression Studies Using qRT-PCR in the White-Backed Planthopper, Sogatella furcifera (Hemiptera: Delphacidae).
Liu et al., Beijing, China. In J Econ Entomol, Dec 2015
In the present study, nine commonly used reference genes, elongation factor 1-α (EF1-α), polyubiquitin (UB), ribosomal protein S18 (RPS18), actin 1 (ACT), α-1 tubulin (TUB), glyceraldehyde-3-phosphate (GAPDH), ribosomal protein L9 (RPL9), ribosomal protein L10 (RPL10), and 18S ribosomal RNA (18S), were evaluated by qRT-PCR for their expression stability under four different experimental conditions (different developmental stages, acquisition of Southern rice black-streaked dwarf virus (SRBSDV), different tissues, and different temperature stress).
Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts.
Kashuba et al., Stockholm, Sweden. In Oncotarget, Sep 2015
We have shown earlier that overexpression of the human mitochondrial ribosomal protein MRPS18-2 (S18-2) led to immortalization of primary rat embryonic fibroblasts.
Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate.
Auburger et al., Frankfurt am Main, Germany. In Neurogenetics, Jul 2015
Quantitative reverse transcriptase PCR in liver tissue validated >1.2-fold upregulations for the ribosomal biogenesis modulator Nop10, the ribosomal components Rps10, Rps18, Rpl14, Rpl18, Gnb2l1, the translation initiation factors Eif2s2, Eif3s6, Eif4b, Pabpc1 and the rER translocase factors Srp14, Ssr1, Sec61b.
Identification of stably expressed reference genes for RT-qPCR data normalization in defined localizations of cyclic bovine ovaries.
Kaessmeyer et al., Berlin, Germany. In Anat Histol Embryol, Jun 2015
Expression profiles of twelve potential reference genes (GAPDH, ACTB, YWHAZ, HPRT1, SDHA, UBA52, POLR2C, RPS9, ACTG2, H3F3B, RPS18 and RPL19) were analysed.
Selection of appropriate reference genes for RT-qPCR analysis in Berkshire, Duroc, Landrace, and Yorkshire pigs.
Kim et al., South Korea. In Gene, Apr 2015
In this study, we assessed the expression stability of 15 well-known candidate reference genes (ACTB, ALDOA, B2M, GAPDH, HPAR1, HSPCB, PGK1, POLR2G, PPIA, RPL4, RPS18, SDHA, TBP, TOP2B, and YWHAZ) in seven body tissues (liver, lung, kidney, spleen, stomach, small intestine, and large intestine) of Berkshire, Landrace, Duroc, and Yorkshire pigs using three excel-based programs, geNorm, NormFinder, and BestKeeper.
Selection of suitable reference genes for normalization of quantitative RT-PCR in peripheral blood samples of bottlenose dolphins (Tursiops truncatus).
Yang et al., Davis, United States. In Sci Rep, 2014
Seventy-five blood samples collected from 7 bottlenose dolphins were used to analyze 15 candidate HKGs (ACTB, B2M, GAPDH, HPRT1, LDHB, PGK1, RPL4, RPL8, RPL18, RPS9, RPS18, TFRC, YWHAZ, LDHA, SDHA).
Transcriptomics profiling and steroid production in mummichog (Fundulus heteroclitus) testes after treatment with 5α-dihydrotestosterone.
Martyniuk et al., Saint John, Canada. In Gen Comp Endocrinol, 2014
While the gene expression levels of actb, efla, rps12, rps18, star, and hsd11b3 remained unchanged, esr2a (esrba), esr2b (esrbb) and cyp11a1 were significantly lower in incubated tissue compared to flash frozen tissue.
Oncolytic reovirus preferentially induces apoptosis in KRAS mutant colorectal cancer cells, and synergizes with irinotecan.
Goel et al., United States. In Oncotarget, 2014
Reovirus efficacy was examined in the KRAS mutant HCT116, and the isogenic KRAS WT Hke3 cell line, and in the non-malignant rat intestinal epithelial cell line.
The complete chloroplast genome sequence of Taxus chinensis var. mairei (Taxaceae): loss of an inverted repeat region and comparative analysis with related species.
Zhang et al., Hangzhou, China. In Gene, 2014
mairei has a large inversion of ~110kb including 91 genes (from rps18 to accD) with gene contents unarranged.
Evaluation of normalization strategies used in real-time quantitative PCR experiments in HepaRG cell line studies.
De Spiegelaere et al., Evergem, Belgium. In Clin Chem, 2014
This study additionally provided clear evidence that the use of a single reference gene [glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein S18 (RPS18), or actin, beta (ACTB)] was insufficient for normalization in HepaRG cells.
Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density.
Singh et al., Karnāl, India. In Reprod Biol Endocrinol, 2013
RESULTS: Three of the genes, GAPDH, HMBS, and HPRT1 were found to be regulated by initial cell plating density, five of them, GAPDH, HMBS, HPRT1, RPLP0 and RPS18 under hypoxic conditions, but none of them after FSH stimulation.
Reference genes selection for quantitative real-time PCR using RankAggreg method in different tissues of Capra hircus.
Bakhtiarizadeh et al., Karaj, Iran. In Plos One, 2012
Since the underlying assumption of reference genes is that expressed at the exact same level in all sample types, in this study, we evaluated the expression stability of nine most commonly used endogenous controls (GAPDH, ACTB, 18S rRNA, RPS18, HSP-90, ALAS, HMBS, ACAC, and B2M) in four different tissues of the domestic goat, Capra hircus, including liver, visceral, subcutaneous fat and longissimus muscles, across different experimental treatments (a standard diet prepared using the NRC computer software as control and the same diet plus one mg chromium/day).
Ribosomal protein S18e as a putative molecular staple for the 18S rRNA 3'-major domain core.
Karpova et al., Russia. In Biochim Biophys Acta, 2011
Ribosomal protein S18e seems to act as a molecular staple fixing the 18S rRNA 3'-major domain core.
MRPS18-2 protein immortalizes primary rat embryonic fibroblasts and endows them with stem cell-like properties.
Szekely et al., Stockholm, Sweden. In Proc Natl Acad Sci U S A, 2009
Data show that S18-2 can immortalize rat embryonic fibroblasts and induces them to express stem cell traits.
Progression-associated genes in astrocytoma identified by novel microarray gene expression data reanalysis.
Lyons-Weiler et al., Washington, D.C., United States. In Methods Mol Biol, 2006
Four genes encoding ribosomal proteins (RPS2, RPS8, RPS18, RPL37A) were upregulated, and five genes (APOD, SORL1, SPOCK2, PRSS11, ID3) were downregulated in high-grade by all tests.
Leader sequence of a plant ribosomal protein gene with complementarity to the 18S rRNA triggers in vitro cap-independent translation.
Van Lijsebettens et al., Gent, Belgium. In Febs Lett, 2006
The RPS18C leader mediated cap-independent translation as demonstrated by dicistronic constructs consisting of luciferase and chloramphenicol acetyl transferase reporter genes in an in vitro wheat germ extract system
Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library.
Suzuki et al., Tokyo, Japan. In Mol Cell Biochem, 2004
cofilin interacts with S18 protein at the actin-binding site
Transcription of ten ribosomal protein genes from tobacco chloroplasts: a compilation of ribosomal protein genes found in the tobacco chloroplast genome.
Sugiura et al., Nagoya, Japan. In Plant Mol Biol, 1988
Transcription of rps2, rps4, rps7, rps11, rps14, rps15, rps18, rpl20, rpl33 and rpl36 from the tobacco chloroplast genome has been studied.
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