miRNAs trigger widespread epigenetically activated siRNAs from transposons in Arabidopsis.
New York City, United States. In Nature, 2014
In plants, post-transcriptional gene silencing (PTGS) is mediated by DICER-LIKE 1 (DCL1)-dependent microRNAs (miRNAs), which also trigger 21-nucleotide secondary short interfering RNAs (siRNAs) via RNA-DEPENDENT RNA POLYMERASE 6 (RDR6), DCL4 and ARGONAUTE 1 (AGO1), whereas transcriptional gene silencing (TGS) of transposons is mediated by 24-nucleotide heterochromatic (het)siRNAs, RDR2, DCL3 and AGO4 (ref.
SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation.
Los Angeles, United States. In Nature, 2014
RNA-directed DNA methylation in Arabidopsis thaliana depends on the upstream synthesis of 24-nucleotide small interfering RNAs (siRNAs) by RNA POLYMERASE IV (Pol IV) and downstream synthesis of non-coding transcripts by Pol V. Pol V transcripts are thought to interact with siRNAs which then recruit DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) to methylate DNA.
Control of female gamete formation by a small RNA pathway in Arabidopsis.
Irapuato, Mexico. In Nature, 2010
Mutations in SUPPRESSOR OF GENE SILENCING 3 and RNA-DEPENDENT RNA POLYMERASE 6 exhibit an identical defect to ago9 mutants, indicating that the movement of small RNA (sRNAs) silencing out of somatic companion cells is necessary for controlling the specification of gametic cells.
Diverse small RNA-directed silencing pathways in plants.
Lubbock, United States. In Biochim Biophys Acta, 2008
These regulatory small RNAs are produced through pathways that involve several evolutionarily conserved protein families, including DICER (DCR) or DICER-LIKE (DCL), ARGONAUTE (AGO), and RNA-DEPENDENT RNA POLYMERASE (RDR).