CO-CBS-H2 S axis: From vascular mediator to cancer regulator.
Tokyo, Japan. In Microcirculation, Dec 2015
We have recently uncovered that stress-inducible CO in and around cancer cells suppresses CBS to result in decreased methylation of 6-phosphofructo-2-kinase/fructose-2,6-bis-phosphatase 3 (PFKFB3), the enzyme regulating phosphofructokinase-1 (PFK-1), leading to a shift of glucose biotransformation from glycolysis towards pentose phosphate pathway; such a metabolic remodeling causes chemoresistance through increasing NADPH and reduced glutathione under stress conditions for cancer cells.
Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.
San Francisco, United States. In Nature, Aug 2015
Phosphofructokinase-1 (PFK1), the 'gatekeeper' of glycolysis, catalyses the committed step of the glycolytic pathway by converting fructose-6-phosphate to fructose-1,6-bisphosphate. Allosteric activation and inhibition of PFK1 by over ten metabolites and in response to hormonal signalling fine-tune glycolytic flux to meet energy requirements.
How do glycolytic enzymes favour cancer cell proliferation by nonmetabolic functions?
Caen, France. In Oncogene, Jul 2015
HKII and phosphoglucose isomerase (PGI) have mainly an antiapoptotic effect; PGI and glyceraldehyde-3-phosphate dehydrogenase activate survival pathways (Akt and so on); phosphofructokinase 1 and triose phosphate isomerase participate in cell cycle activation; aldolase promotes epithelial mesenchymal transition; PKM2 enhances various nuclear effects such as transcription, stabilisation and so on.
T-cell metabolism in autoimmune disease.
Stanford, United States. In Arthritis Res Ther, 2014
This anti-Warburg effect results from insufficient activity of the glycolytic enzyme phosphofructokinase and differentiates the metabolic status in RA T cells from those in cancer cells.
The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4.
Seoul, South Korea. In Nat Biotechnol, 2005
The genome lacks recognizable genes for 6-phosphofructokinase, an essential enzyme in the Embden-Meyerhof-Parnas pathway, and for two enzymes in the tricarboxylic acid cycle, the 2-oxoglutarate dehydrogenase complex and malate dehydrogenase, so glucose can be metabolized only by the Entner-Doudoroff pathway.