Studying protein fold evolution with hybrids of differently folded homologs.
Tucson, United States. In Protein Eng Des Sel, Aug 2015
To study the sequence determinants governing protein fold evolution, we generated hybrid sequences from two homologous proteins with 40% identity but different folds: Pfl 6 Cro, which has a mixed α + β structure, and Xfaso 1 Cro, which has an all α-helical structure.
Ethanol production by engineered thermophiles.
United States. In Curr Opin Biotechnol, Jun 2015
Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogenase (PDH), pyruvate formate lyase (PFL), and pyruvate ferredoxin oxidoreductase (PFOR).
Glycyl radical activating enzymes: structure, mechanism, and substrate interactions.
Bozeman, United States. In Arch Biochem Biophys, 2014
The GRE-AE pyruvate formate lyase activating enzyme (PFL-AE) is extensively characterized and has provided insights into the active site structure of radical SAM enzymes including GRE-AEs, illustrating the nature of the interactions with their corresponding substrate GREs and external electron donors.
Fermentative Pyruvate and Acetyl-Coenzyme A Metabolism.
In Ecosal Plus, 2004
Consequently, in the absence of an electron acceptor mutants lacking either branch of acetyl-CoA metabolism fail to cleave pyruvate, despite the presence of PFL, and instead reduce it to D-lactate by the D-lactate dehydrogenase.
[Vascular-specific promoters and cis-regulatory elements].
Beijing, China. In Sheng Wu Gong Cheng Xue Bao, 2003
The cis-elements and motifs have been studied in detail for the promoters of bean phenylalanine ammonia lyase (PAL2), bean glycine-rich protein (grp 1.8) and Arabidopsis profilin2 (pfn2) in particular.The motif of vs-1 (CATGCTCCGTTGGATGTGGAAGACAGCA) found in grp 1.8 promoter was a cis-element that specificically bind to a transcription activation factor VSF-1 protein (one of the bZIP proteins).