A novel role for RhoA GTPase in the regulation of airway smooth muscle contraction.
Indianapolis, United States. In Can J Physiol Pharmacol, Feb 2015
Contractile stimulation of ASM induces the recruitment and assembly of paxillin, vinculin, and focal adhesion kinase (FAK) into membrane adhesion complexes (adhesomes) that regulate actin polymerization by catalyzing the activation of cdc42 GTPase by the G-protein-coupled receptor kinase-interacting target (GIT) - p21-activated kinase (PAK) - PAK-interacting exchange factor (PIX) complex.
Nanoscale architecture of integrin-based cell adhesions.
Bethesda, United States. In Nature, 2010
Our results reveal that integrins and actin are vertically separated by a ∼40-nm focal adhesion core region consisting of multiple protein-specific strata: a membrane-apposed integrin signalling layer containing integrin cytoplasmic tails, focal adhesion kinase and paxillin; an intermediate force-transduction layer containing talin and vinculin; and an uppermost actin-regulatory layer containing zyxin, vasodilator-stimulated phosphoprotein and α-actinin.
A distinctive role for focal adhesion proteins in three-dimensional cell motility.
More papers using
Baltimore, United States. In Nat Cell Biol, 2010
Live-cell microscopy shows that for cells fully embedded in a 3D matrix, focal adhesion proteins, including vinculin, paxillin, talin, alpha-actinin, zyxin, VASP, FAK and p130Cas, do not form aggregates but are diffusely distributed throughout the cytoplasm.
Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
In The Journal of Cell Biology, 2003
... Commercial antibody sources included ICN Biomedicals (α-actinin), Sigma-Aldrich (talin and vinculin), BD Biosciences (caldesmon, ILK, paxillin, and integrins α3, αV, β1, β3), Upstate Cell Signaling (FAK 4–47, pY-4G10, and ... Camp-sensitive and rapamycin-sensitive regulation of the association of eukaryotic initiation-factor 4e and the translational regulator Phas-I in aortic smooth-muscle cells
In Journal of Molecular and Cellular Cardiology, 1994
... hyperphosphorylated-Rb, phospho-ERK, ERK, phospho-JNK, JNK (#9308, #9101, #9102, #9251, #9258; Cell Signalling), V5-tag (#R960-25; Invitrogen), paxillin (#610051; BD Biosciences), BrdU (#B2531; Sigma) and ...