Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation.
In Ukr Biochem J, 2015
This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as fluorescent photoswitches, fluorescence recovery after photobleaching (FRAP), fluorescent resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), stimulated emission depletion (STED), total internal reflection fluorescence (TIRF) and other techiques.
Challenges in quantitative single molecule localization microscopy.
Lausanne, Switzerland. In Febs Lett, 2014
Due to its advantages in labeling specificity and the relatively low overcounting caused by photoblinking when photo-activable fluorescent proteins (PA-FPs) are used as labels, we focus specifically on Photo-Activated Localization Microscopy (PALM), even though the ideas presented might be applicable to SMLM in general.
Multifunctional enveloped nanodevices (MENDs).
Sapporo, Japan. In Adv Genet, 2013
In this chapter, we review the development and evolution of the MEND by providing several successful examples, including the R8-MEND, the KALA-MEND, the MITO-Porter, the YSK-MEND, and the PALM.
Palmitoylation stabilizes unliganded rod opsin.
Cleveland, United States. In Proc Natl Acad Sci U S A, 2010
report that palmitoylation-deficient (Palm(-/-)) mice carrying two Cys to Thr and Ser mutations in the opsin gene displayed profound light-induced retinal degeneration that first involved rod and then cone cells.