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GoPubMed Proteins lists recent and important papers and reviews for proteins. Page last changed on 30 Jul 2015.

Cyclin-dependent kinase inhibitor 1A

p21, Cyclin-Dependent Kinase Inhibitor p21, CIP1, K-ras, WAF1, H-ras
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010] (from NCBI)
Top mentioned proteins: p53, PCNA, V1a, CAN, p27
Papers using p21 antibodies
Arecoline downregulates levels of p21 and p27 through the reactive oxygen species/mTOR complex 1 pathway and may contribute to oral squamous cell carcinoma
Li Ji-Cheng et al., In International Journal of Biological Sciences, 2011
... P21 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) ...
Aaptamine, an alkaloid from the sponge Aaptos suberitoides, functions as a proteasome inhibitor
Kong Dexin et al., In International Journal of Molecular Sciences, 2009
... Anti-Cip1/WAF-1/p21 antibody was from Milipore.
Ras-related small GTPases RalA and RalB regulate cellular survival after ionizing radiation
Rodemann H Peter et al., In Breast Cancer Research : BCR, 2009
... Inhibitors against PI3K (LY294002), MEK (PD98059) and anti-K-Ras antibody were purchased from Merck Biosciences (Darmstadt, Germany) ...
GSK3beta: role in therapeutic landscape and development of modulators
Yoo Lina I et al., In BMC Urology, 2009
... The monoclonal p21 antibody was obtained from BD Biosciences (San Diego, CA) ...
Weak p53 permits senescence during cell cycle arrest.
El-Deiry Wafik S., In PLoS ONE, 2009
... HT1080-p21-9 cells were cultured in high-glucose DMEM without puruvate supplemented with FC2 serum (HyClone FetalClone II from Thermo Scientific, Logan, Utah) ...
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Papers on p21
Keap1-Nrf2 signalling in pancreatic cancer.
Charnley et al., Edinburgh, United Kingdom. In Int J Biochem Cell Biol, 31 Aug 2015
Upregulation of Nrf2 is, at least in part, K-Ras oncogene-driven and contributes to pancreatic cancer proliferation and chemoresistance.
Hepatic progenitor cells of biliary origin with liver repopulation capacity.
Forbes et al., Niigata, Japan. In Nat Cell Biol, 20 Aug 2015
Here, we describe a mouse model where the E3 ubiquitin ligase Mdm2 is inducibly deleted in more than 98% of hepatocytes, causing apoptosis, necrosis and senescence with nearly all hepatocytes expressing p21.
A high-throughput kinome screen reveals serum/glucocorticoid-regulated kinase 1 as a therapeutic target for NF2-deficient meningiomas.
Ramesh et al., Boston, United States. In Oncotarget, 10 Aug 2015
Among the emerging top candidates were the mTORC2-specific target serum/glucocorticoid-regulated kinase 1 (SGK1) and p21-activated kinase 1 (PAK1).
Toll like receptors and pancreatic diseases: From a pathogenetic mechanism to a therapeutic target.
Cascinu et al., Ancona, Italy. In Cancer Treat Rev, 31 Jul 2015
Furthermore, TLRs cause derangements in several tumor suppressor proteins (such as p16, p21, p27, p53 and pRb), induce STAT3 activation and promote epithelial-mesenchymal transition as well as oncogene-induced senescence.
Initiation and termination of DNA replication during S phase in relation to cyclins D1, E and A, p21WAF1, Cdt1 and the p12 subunit of DNA polymerase δ revealed in individual cells by cytometry.
Zhang et al., Valhalla, United States. In Oncotarget, 20 Jun 2015
During our recent studies on mechanism of the regulation of human DNA polymerase δ in preparation for DNA replication or repair, multiparameter imaging cytometry as exemplified by laser scanning cytometry (LSC) has been used to assess changes in expression of the following nuclear proteins associated with initiation of DNA replication: cyclin A, PCNA, Ki-67, p21(WAF1), DNA replication factor Cdt1 and the smallest subunit of DNA polymerase δ, p12.
How do K-RAS-activated cells evade cellular defense mechanisms?
Bae et al., Ch'ŏngju, South Korea. In Oncogene, 11 Jun 2015
Numerous studies have shown that K-RAS mutation is among the most important early events in carcinogenesis of the lung.
TGF-β promotes heterogeneity and drug resistance in squamous cell carcinoma.
Fuchs et al., New York City, United States. In Cell, Mar 2015
Rather, TGF-β transcriptionally activates p21, which stabilizes NRF2, thereby markedly enhancing glutathione metabolism and diminishing effectiveness of anti-cancer therapeutics.
Cdk1-dependent mitotic enrichment of cortical myosin II promotes cell rounding against confinement.
Muller et al., Basel, Switzerland. In Nat Cell Biol, Feb 2015
Our data suggest that progressive accumulation of myosin II to the mitotic cell cortex probably requires the Cdk1 activation of both p21-activated kinases, which inhibit myosin recruitment, and of Rho kinase, which stimulates myosin recruitment to the cortex.
Bugs and food: a recipe for cancer?
Jobin et al., Gainesville, United States. In Cell Metab, Jan 2015
Recently, Schulz et al. (2014) demonstrated that high-fat diet-induced intestinal dysbiosis promotes cancer development in K-ras(G12Dint) mice without inducing obesity or mucosal inflammation, positioning microbial activities as a central component of diet-induced carcinogenesis.
MDC1 Enhances Estrogen Receptor-mediated Transactivation and Contributes to Breast Cancer Suppression.
Zhao et al., Shenyang, China. In Int J Biol Sci, Dec 2014
Knockdown of MDC1 reduced the transcription of the endogenous ERα target genes, including p21.
Potential Effects of Pomegranate Polyphenols in Cancer Prevention and Therapy.
Fimognari et al., Rimini, Italy. In Oxid Med Cell Longev, Dec 2014
Pomegranate evokes antiproliferative, anti-invasive, and antimetastatic effects, induces apoptosis through the modulation of Bcl-2 proteins, upregulates p21 and p27, and downregulates cyclin-cdk network.
Biological effects of ray cartilage extract on human breast cancer cell line MCF-7 and its mechanism.
Li et al., Jinzhou, China. In Int J Clin Exp Med, Dec 2014
METHODS: MCF-7 cells were treated with RCE of different concentrations for different durations, and then MCF-7 cell proliferation was evaluated with MTT test, cell cycle was detected with flow cytometer and the protein levels of cyclin D1 and p21 were determined with Western blot.
Honokiol induces cell cycle arrest and apoptosis via p53 activation in H4 human neuroglioma cells.
Liu et al., Changchun, China. In Int J Clin Exp Med, Dec 2014
Moreover, p53, p21 and Bax/Bcl-2 were significantly upregulated by honokiol treatment.
Schisandrin B inhibits the proliferation of human lung adenocarcinoma A549 cells by inducing cycle arrest and apoptosis.
Zhang et al., Changchun, China. In Int J Clin Exp Med, Dec 2014
Sch B induced cell cycle arrest at G0/G1 phase by down-regulating the expression of cyclin D1, cyclin-dependent kinase (CDK)4, and CDK6, but up-regulating p53 and p21 expression in A549 cells.
A functional genomic approach identifies FAL1 as an oncogenic long noncoding RNA that associates with BMI1 and represses p21 expression in cancer.
Zhang et al., Philadelphia, United States. In Cancer Cell, Oct 2014
FAL1 associates with the epigenetic repressor BMI1 and regulates its stability in order to modulate the transcription of a number of genes including CDKN1A.
The intermediate-activity (L597V)BRAF mutant acts as an epistatic modifier of oncogenic RAS by enhancing signaling through the RAF/MEK/ERK pathway.
Pritchard et al., Leicester, United Kingdom. In Genes Dev, 2012
We combined (L597V)Braf with (G12D)Kras and found that (L597V)Braf modified (G12D)Kras oncogenesis such that fibroblast transformation and lung tumor development were more reminiscent of that driven by the high-activity (V600E)Braf mutant
Autophagic activity dictates the cellular response to oncogenic RAS.
Lu et al., Oxford, United Kingdom. In Proc Natl Acad Sci U S A, 2012
ASPP2 modulates oncogenic RAS-induced autophagic activity to dictate the cellular response to RAS: to proliferate or senesce.
K-Ras and B-Raf oncogenes inhibit colon epithelial polarity establishment through up-regulation of c-myc.
Hall et al., New York City, United States. In J Cell Biol, 2012
It was concluded that extracellular kinase-mediated up-regulation of c-myc by K-Ras or B-Raf oncogenes disrupts the establishment of apical/basolateral polarity in colon epithelial cells independently of its effect on proliferation.
[What is the prognostic importance of molecular genetic factors in endometrial carcinoma?].
Spaček et al., United Kingdom. In Klin Onkol, 2011
possible role of K- ras mutation as an early event in the process of endometrial carcinogenesis in type 1 tumors
[Small molecules--principal participants of pathogenesis of rheumatoid arthritis].
Dubikov et al., In Klin Med (mosk), 2011
[review] Small molecules like p21 and PUMA may play a role in the pathogenesis of rheumatoid arthritis.
More papers using p21 antibodies
Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors
Li Long-Cheng et al., In Molecular therapy. Nucleic acids, 2008
... Immunodetection proceeded by incubating slides overnight at 4 °C with primary antibody specific to p21 (Cell Signaling Technology, Danvers, MA) diluted ...
A dual role of p53 in the control of autophagy.
Abdel-Naim Ashraf B., In PLoS ONE, 2007
... Primary antibodies, including p53, phospho-p53 (Ser15), phospho-p53 (Thr55), p21, sequestosome 1 (SQSTM1/p62), and survivin, were purchased from Santa Cruz Biotechnology, Santa Cruz, CA ...
DEC1, a basic helix-loop-helix transcription factor and a novel target gene of the p53 family, mediates p53-dependent premature senescence
Swarbrick Alexander, In PLoS ONE, 2007
... Rabbit anti-p53(FL-393), mouse anti-p63(4A4), rabbit anti-GADD45α(C-20) and rabbit anti-p21(C-19) were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA) ...
Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin
Cook John A. et al., In Frontiers in Oncology, 2007
... (Fremont, CA, USA); rabbit polyclonal IGF-1Rβ, p21 antibodies, and PARP-1 (F-2) were from Santa Cruz Biotechnology, Inc ...
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Malle Ernst et al., In DNA Repair, 2007
... , termed γ-H2AX, Cell Signaling Technology, Beverly, MA, USA); (vi) rabbit monoclonal anti-p21 Waf1/Cip1 antibody (12D1, Cell Signaling Technology, Beverly, MA, USA), ...
Biological roles of estrogen and progesterone in human endometrial carcinoma—new developments in potential endocrine therapy for endometrial cancer
DeMayo Francesco J. et al., In Journal of Oncology, 2006
... K-ras mutation, total RNA was isolated from uterus according to, Qiagen minieasy kit protocol ...
Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
Deb Sumitra, In PLoS ONE, 2006
... Anti-human MDM2 (SMP14) and p21 (C19) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Stopping ras in its tracks.
Cebecauer Marek, In PLoS ONE, 2006
... Mouse anti-pan-Ras (Ab-3) and mouse monoclonal anti-K-Ras antibodies (mAbs) were obtained from Calbiochem, rabbit anti-β-tubulin antibodies (Abs) from Santa Cruz Biotechnology, mouse anti-phospho-ERK Ab from ...
Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion
Arlt Alexander, In PLoS ONE, 2005
... Anti-luciferase antibody came from Promega, anti-nucleolin and anti-IκB antibody from Santa Cruz Biotechnology, anti-HIF-1α and anti-p21 antibody were from BD Biosciences.
The anti-angiogenic peptide anginex disrupts the cell membrane
Yang K-D et al., In Cell Death & Disease, 2005
... Antibodies to â-actin, H-Ras, and K-Ras were purchased from Abcam Cambridge Science Park (Cambridge, ...
The crystal structure of yeast protein disulfide isomerase suggests cooperativity between its active sites
Townsend Danyelle M. et al., In International Journal of Cell Biology, 2005
... (Millipore), and anti-c-Myc/anti-Cyclin D1/anti-p21 (Cell Signaling).
Integrin-mediated transforming growth factor-beta activation regulates homeostasis of the pulmonary epithelial-mesenchymal trophic unit
Kuwano Kazuyoshi et al., In Oncoimmunology, 2005
... Antibodies used were rabbit anti-LC3 (Novus), rabbit anti-ATG5 (Novus), mouse anti-p21 (Cell signaling Technology), rabbit anti-phospho-histone H2A.X ...
The structure and regulation of vinculin
Beaulieu Jean-François et al., In Journal of Cellular Physiology, 2005
... ON), α5 integrin (WB: 1/1,000) (AB1928, Millipore, Temecula, CA), αV integrin (WB: 1/1,000) (AB1930, Millipore), p21 (WB: 1/2,000) (Santa Cruz Biotechnology, Inc.), p27 (WB: 1/2,000) ...
Hsp70-2 is required for tumor cell growth and survival
O'Rand Michael G et al., In Reproductive Biology and Endocrinology : RB&E, 2004
... Mouse monoclonal anti p53 antibody (DO-1) and rabbit polyclonal anti-p21 antibody (SC-397) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) ...
Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis
Wang X et al., In Cell Death and Differentiation, 2004
... Autophagy antibody kits and p21 antibody were from Cell Signaling (Beverly, MA, USA) ...
Image Processing with ImageJ
Goldfinger Lawrence E. et al., In Small GTPases, 2003
... α-R-Ras, α-H-Ras, α-GFP and α-p110α antibodies were purchased from Santa Cruz Biotechnology.
Comparison of the effect of mitochondrial inhibitors on mitochondrial membrane potential in two different cell lines using flow cytometry and spectrofluorometry
Manié S N et al., In Cell Death & Disease, 2002
... Santa-Cruz, Le perray en Yvelines, France), anti-ATG5 (D1G9, Cell Signaling, Montigny le Bretonneux, France), anti-p21 Waf1/Cip1 (DCS60, Cell Signaling), anti-Raptor (24C12, Cell Signaling), ...
Requirement for Ras Guanine Nucleotide Releasing Protein 3 in Coupling Phospholipase C-γ2 to Ras in B Cell Receptor Signaling
Kurosaki Tomohiro et al., In The Journal of Experimental Medicine, 2002
... Anti–H-Ras Ab (C-20) and anti–K-Ras Ab (F234) were purchased from Santa Cruz Biotechnology, Inc ...
BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways
Dobbelstein Matthias et al., In The Journal of Cell Biology, 2002
... and siRNA control #2, were purchased from Ambion (with the exception of the siRNA to p21, which was purchased from Santa Cruz Biotechnology, Inc.) and are described ...
Mitomycin C induces apoptosis and caspase-8 and −9 processing through a caspase-3 and Fas-independent pathway.
Hong Show-Jen et al., In Molecular Vision, 2001
... and p21 were purchased from Santa Cruz Biotechnology Inc ...
Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 gene.
Nelson Jay A., In PLoS Pathogens, 2001
... α-MCM4 (BD Pharmingen), α-lamin A (Biolegend), α-cdc6 (Upstate), α-cyclin B1 (Thermo Scientific), α-cyclin A, α-geminin, α-p53, α-p21 (all four from Santa Cruz Biotechnology).
Reovirus as an oncolytic agent against experimental human malignant gliomas
Lee P W K et al., In British Journal of Cancer, 2000
... -actin, p65, PUMA, Bax and p21 were purchased from Santa Cruz Biotechnology Inc ...
Loss of p53 promotes RhoA–ROCK-dependent cell migration and invasion in 3D matrices
Roux Pierre et al., In The Journal of Cell Biology, 1999
... The primary antibodies used were anti-p21 (C-19; Santa Cruz Biotechnology, Inc.), anti-p53 (DO-1; Novocastra), ...
Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Sessa William C. et al., In The Journal of Cell Biology, 1999
... Antibodies against PECAM-1 (clone M-20), p16 (clone C-20), and p21 (clone C-19) were obtained from Santa Cruz Biotechnology, Inc ...
Regulation of the MEF2 family of transcription factors by p38
Rotwein Peter et al., In The Journal of Cell Biology, 1998
... Antibodies to p21 and CDK-4 used for immunoblotting were purchased from Santa Cruz Biotechnology, Inc ...
mdm-2 gene amplification in 3T3-L1 preadipocytes
Berberich Steven J. et al., In Aging (Albany NY), 1998
... HdmX polyclonal antibody (Bethyl Laboratories, Inc.), p21 polyclonal antibody C-19 (Santa Cruz Biotechnology, Inc.), p53 ...
Corticothalamic connections of extrastriate visual areas in rhesus monkeys
Levitt Pat et al., In Cerebral Cortex (New York, NY), 1996
... Briefly, P0 brains were sectioned at 20 μM with a cryostat, and P7–P21 brains were sectioned at 40 μM with a freezing sliding microtome (Leica).
Subregion- and cell type-restricted gene knockout in mouse brain.
Albertson Dawn N., In PLoS ONE, 1995
... pThyLSL-EGFP-F was created by cloning the fragment encoding EGFP fused to the H-Ras farnesylation site from pEGFP-F (BD Biosciences, Mountain View, CA) into ...
Specific p53 mutations are associated with de novo resistance to doxorubicin in breast cancer patients
Daoud Sayed S et al., In Breast Cancer Research, 1995
... Primary antibodies against p53 and p21 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); Hsp ...
Stages of embryonic development of the zebrafish.
Hotchin Neil, In PLoS ONE, 1994
... The following antibodies were used: Goat polyclonal antibody against the NRP-1-C-terminal domain, anti-GIPC, anti-p53 and anti-p21 were purchased from Santa Cruz Biotechnology, Inc ...
TGFβ induces SIK to negatively regulate type I receptor kinase signaling
Moustakas Aristidis et al., In The Journal of Cell Biology, 1993
... and anti-Flag (M5) antibodies were obtained from Sigma-Aldrich; anti-GFP (A11122) from Invitrogen; anti–PAI-1 and anti-p21 (Cip1/WAF1) from BD Biosciences; anti-HA (Y-11), anti-TβRI (V-22), ...
MI-219-Zinc Combination: a new Paradigm in MDM2 Inhibitor Based Therapy
Mohammad Ramzi M. et al., In Oncogene, 1993
... Primary antibodies for p53, Bax, MDM2 and p21 were purchased from Cell Signaling (Beverly MA) ...
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