ORC proteins in the mammalian zygote.
Honolulu, United States. In Cell Tissue Res, Jan 2016
The origin recognition complex (ORC) proteins, ORC1-6, are the first known proteins that bind DNA replication origins to mark the competency for the initiation of DNA synthesis.
Crystal structure of the eukaryotic origin recognition complex.
Baltimore, United States. In Nature, Apr 2015
Comparative analyses indicate that ORC encircles DNA, using its winged-helix domain face to engage the mini-chromosome maintenance 2-7 (MCM2-7) complex during replicative helicase loading; however, an observed out-of-plane rotation of more than 90° for the Orc1 AAA+ domain disrupts interactions with catalytic amino acids in Orc4, narrowing and sealing off entry into the central channel.
Nijmegen, Netherlands. In Orphanet J Rare Dis, 2014
Mutations in one of five genes (ORC1, ORC4, ORC6, CDT1, and CDC6) of the pre-replication complex, involved in DNA-replication, are detected in approximately 67-78% of patients with MGS.
DNA-protein interaction dynamics at the Lamin B2 replication origin.
Pisa, Italy. In Cell Cycle, 2014
In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4.
A role for DNA polymerase θ in the timing of DNA replication.
Toulouse, France. In Nat Commun, 2013
We find that Pol θ binds to chromatin during early G1, interacts with the Orc2 and Orc4 components of the Origin recognition complex and that the association of Mcm proteins with chromatin is enhanced in G1 when Pol θ is downregulated.
Mechanisms and pathways of growth failure in primordial dwarfism.
Edinburgh, United Kingdom. In Genes Dev, 2011
Ten genes have now been identified for microcephalic primordial dwarfism, encoding proteins involved in fundamental cellular processes including genome replication (ORC1 [origin recognition complex 1], ORC4, ORC6, CDT1, and CDC6), DNA damage response (ATR [ataxia-telangiectasia and Rad3-related]), mRNA splicing (U4atac), and centrosome function (CEP152, PCNT, and CPAP).