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Neurofilament, heavy polypeptide

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] (from NCBI)
Top mentioned proteins: INa, NF-L, CAN, HAD, ACID
Papers using NF-H antibodies
Neurofilament heavy chain side arm phosphorylation regulates axonal transport of neurofilaments
Miller Christopher C.J. et al., In The Journal of Cell Biology, 1997
... Amino-terminal GFP-tagged rat NFH was prepared by cloning the cDNA into pEGFP-C3 (CLONTECH Laboratories, Inc.).
An essential cytoskeletal linker protein connecting actin microfilaments to intermediate filaments
Liem Ronald K.H. et al., In The Journal of Cell Biology, 1995
... expression cassette of NFH, was isolated and ligated to the BamHI digested pCI-NFL to yield pCI-NFL/NFH.Two-hybrid and three-hybrid constructs were made with plasmids pGBT9, pGAD424, pAS2-1, and pACT2 (Clontech ...
Selective effects of experimental glaucoma on axonal transport by retinal ganglion cells to the dorsal lateral geniculate nucleus
Takada Masahiko et al., In The British Journal of Ophthalmology, 1990
... monoclonal mouse antibody (SMI-31; Sternberger Monoclonal Inc., Lutherville, Maryland, USA) or non-phosphorylated neurofilament (SMI-32), neurofilament-H (NF-H; Biomol International, LP, Plymouth Meeting, ...
Papers on NF-H
Promise of Retinoic Acid-Triazolyl Derivatives in Promoting Differentiation of Neuroblastoma Cells.
Bhat et al., Jammu, India. In Acs Chem Neurosci, Feb 2016
Further on, to confirm this differentiation potential of these compounds, neuroblastoma cells were probed for expression of neuronal markers such as NF-H and NeuN.
Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms.
Xie et al., Jinan, China. In Neurochem Res, Jan 2016
Furthermore, ACR intoxication significantly increases the protein levels of the three neurofilament subunits (NF-L, NF-M, NF-H), kinesin, dynein, and dynamitin subunit of dynactin in DRG neurons.
Assessment of Subclinical Atherosclerosis Using Computed Tomography Calcium Scores in Patients with Familial and Nonfamilial Hypercholesterolemia.
Gruchala et al., Gdańsk, Poland. In J Atheroscler Thromb, Jan 2016
AIM: The aim of this study was to compare coronary calcium scores and aortic calcium scores between patients with severe hypercholesterolemia having a DNA-based diagnosis of FH (FH group) versus patients with severe hypercholesterolemia without the FH gene mutation (NFH group).
Neuroprotective Effect of Calpeptin on Acrylamide-Induced Neuropathy in Rats.
Yu et al., Jinan, China. In Neurochem Res, Nov 2015
Compared with control group, body weight of rats in ACR group decreased by 11.3 % (P < 0.01), while in ACR + CP group body weight increased significantly by 8.3 % (P < 0.01) compared with ACR group by the end of the 4th week; gait score of rats in both ACR and ACR + CP groups increased significantly by 167 % and 100 % (P < 0.01) compared with control group, while it decreased significantly by 25.1 % (P < 0.01) in ACR + CP group compared with ACR group; the distance of hind limb splay in both ACR and ACR + CP groups increased by 76.7 % and 49.5 % (P < 0.01) compared with control group, while it decreased by 15.4 % (P < 0.01) in ACR + CP group compared with ACR group; calpain activity of spinal cord at ACR and ACR + CP groups increased significantly by 14.9 % and 10.0 % (P < 0.01) compared with control group, while it decreased 4.2 % (P < 0.01) in ACR + CP group compared with ACR group; compared with control group, the levels of light NF (NF-L), medium NF (NF-M) and heavy NF (NF-H) subunits increased by 81.2 %, 263.6 % and 22.6 % (P < 0.01) in the supernatant of ACR group in spinal cord tissue and increased by 28.4 %, 96.6 % and 10.6 % (P < 0.01) in ACR + CP group, while the levels of NF-L, NF-M and NF-H subunits decreased by 29.1 %, 45.9 % and 9.8 % (P < 0.01) in ACR + CP group compared with ACR group.
Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate into Motor Neurons on Electrospun Poly(ε-caprolactone) Scaffold.
Ai et al., Tehrān, Iran. In Mol Neurobiol, Oct 2015
Engineered hBM-MSCs and hEnSCs seeded on PCL nanofibrous scaffold were differentiated into beta-tubulin III, islet-1, Neurofilament-H (NF-H), HB9, Pax6, and choactase-positive motor neurons by immunostaining and real-time PCR, in response to the signaling molecules.
IL-10 Protects Neurites in Oxygen-Glucose-Deprived Cortical Neurons through the PI3K/Akt Pathway.
Liu et al., Fuzhou, China. In Plos One, 2014
After staining NF-H and GAP-43, we found that IL-10 significantly protected neurites in terms of axon length and dendrite number by confocal microscopy.
Brain injury biomarkers as outcome predictors in pediatric severe traumatic brain injury.
Fraser et al., London, Canada. In Neurocrit Care, 2014
Significant correlations were established between sTBI outcomes and various biomarkers in CSF (IL-6, IL-8, IL-1β, S100β, NGF, NSE, DCX, ET-1, HMGB-1, cytochrome C) and blood (GFAP, NF-H, UCH-L1, SBDP-145, leptin).
Axonal degeneration in multiple sclerosis: can we predict and prevent permanent disability?
Petratos et al., In Acta Neuropathol Commun, 2013
Greater accuracy of predictive biomarkers is therefore warranted and may include CSF neurofilament light chain (NF-L) and neurofilament heavy chain (NF-H) levels, for progressive MS.
Structures and interactions in 'bottlebrush' neurofilaments: the role of charged disordered proteins in forming hydrogel networks.
Safinya et al., Tel Aviv-Yafo, Israel. In Biochem Soc Trans, 2012
NFs (neurofilaments), the major cytoskeletal constituent of myelinated axons in vertebrates, consist of three different molecular-mass subunit proteins, NF-L (low), NF-M (medium) and NF-H (high), assembled to form mature filaments with protruding intrinsically disordered C-terminal side-arms.
The involvement of neurofilament heavy chain phosphorylation in the maturation and degeneration of rat oocytes.
Ishizuka et al., Kawasaki, Japan. In Endocrinology, 2012
NF-H expressed in oocytes may be involved in the maintenance of oocyte structure during follicular growth.
The usefulness of S100B, NSE, GFAP, NF-H, secretagogin and Hsp70 as a predictive biomarker of outcome in children with traumatic brain injury.
Fedora et al., Brno, Czech Republic. In Acta Neurochir (wien), 2012
NF-H levels increase significantly faster in children who have a worse Glasgow Outcome Scale following traumatic brain injury, or died.
Proteomic biomarker discovery in cerebrospinal fluid for cerebral vasospasm following subarachnoid hemorrhage.
Steinberg et al., Stanford, United States. In J Stroke Cerebrovasc Dis, 2012
Category A markers included tumor necrosis factor (TNF)-α, soluble tumor necrosis factor receptor I (sTNFR-I), and interleukin (IL)-1 receptor antagonist (IL-1ra), as well as the neurofilament proteins NFL and NfH.
Hyperacute detection of neurofilament heavy chain in serum following stroke: a transient sign.
Petzold et al., München, Germany. In Neurochem Res, 2011
significant in vivo information on the pathophysiology of stroke may be obtained by the determination of NfH(SMI35).
Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro.
Li et al., Jinan, China. In Peptides, 2011
Neuregulin-1beta regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro.
Soluble beta-amyloid precursor protein is related to disease progression in amyotrophic lateral sclerosis.
Brettschneider et al., Ulm, Germany. In Plos One, 2010
Data suggest that a deficiency of cellular neuroprotective mechanisms (decrease of sAPP) is linked to progressive neuro-axonal damage (increase of NfH(SMI35)) and to progression of disease.
Gel-expanded to gel-condensed transition in neurofilament networks revealed by direct force measurements.
Safinya et al., Santa Barbara, United States. In Nat Mater, 2010
Neurofilaments (NF)--the principal cytoskeletal constituent of myelinated axons in vertebrates--consist of three molecular-weight subunit proteins NF-L (low), NF-M (medium) and NF-H (high), assembled to form mature filaments with protruding unstructured C-terminus side arms.
Neuronal intermediate filaments.
Cleveland et al., Baltimore, United States. In Annu Rev Neurosci, 1995
Assembled as obligate heteropolymers requiring NF-L and substoichiometric amounts of NF-M and/or NF-H, NF investment into axons is essential for establishment of axonal caliber, itself a key determinant of conduction velocity.
Defective axonal transport in a transgenic mouse model of amyotrophic lateral sclerosis.
Julien et al., Montréal, Canada. In Nature, 1995
First, transgenic mice that overexpress neurofilament proteins show motor neuron degeneration and, second, variant alleles of the neurofilament heavy-subunit gene (NF-H) have been found in some human ALS patients.
Progressive neuronopathy in transgenic mice expressing the human neurofilament heavy gene: a mouse model of amyotrophic lateral sclerosis.
Julien et al., Canada. In Cell, 1993
We generated four transgenic mice with a 34 kb genomic fragment including the complete human neurofilament heavy (NF-H) gene.
The Use and Potential of pNF-H as a General Blood Biomarker of Axonal Loss: An Immediate Application for CNS Injury
Shaw, Boca Raton, United States. In Unknown Journal, 0001
This chapter describes the unusual protein chemical properties of the phosphorylated axonal form of the heavy neurofilament subunit NF-H (pNF-H).
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