myosin light chain 2
myosin light chain 2
Mutation Analysis of the Main Hypertrophic Cardiomyopathy Genes Using Multiplex Amplification and Semiconductor Next-Generation Sequencing.
Davao, Philippines. In Circ J, 22 Nov 2014
The purpose was to develop a next-generation sequencing (NGS) procedure for the main HCM genes.Methods and Results:Multiplex amplification of the coding exons ofMYH7,MYBPC3,TNNT2,TNNI3,ACTC1,TNNC1,MYL2,MYL3, andTPM1was designated, followed by NGS with the Ion Torrent PGM (Life Technologies).
Proteomic Analysis of Human Fetal Atria and Ventricle.
In J Proteome Res, 17 Nov 2014
Several previously characterised cardiac chamber-enriched proteins were identified in this study including atrial isoform of myosin light chain 2 (MYL7), atrial natriuretic peptide (NPPA), connexin 40 (GJA5), and peptidylglycine alpha-amidating monooxygenase (PAM) for atria, and ventricular isoforms of myosin light chains (MYL2 and MYL3), myosin heavy chain 7 (MYH7), and connexin 43 (GJA1) for ventricle.
High efficiency differentiation of human pluripotent stem cells to cardiomyocytes and characterization by flow cytometry.
Hong Kong, Hong Kong. In J Vis Exp, Dec 2013
In contrast to many previous methods, the cardiomyocyte differentiation protocol described here does not require cell aggregation or the addition of Activin A or BMP4 and robustly generates cultures of cells that are highly positive for cardiac troponin I and T (TNNI3, TNNT2), iroquois-class homeodomain protein IRX-4 (IRX4), myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform (MLC2a) by day 10 across all human embryonic stem cell (hESC) and hiPSC lines tested to date.
Molecular pathways: MERTK signaling in cancer.
Chapel Hill, United States. In Clin Cancer Res, Nov 2013
These include the mitogen-activated protein kinase and phosphoinositide 3-kinase pathways, as well as regulation of signal transducer and activator of transcription family members, migration-associated proteins including the focal adhesion kinase and myosin light chain 2, and prosurvival proteins such as survivin and Bcl-2.