Knockdown of Lmo7 inhibits chick myogenesis.
Rio de Janeiro, Brazil. In Febs Lett, 20 Feb 2016
Knockdown of Lmo7 using siRNA specific to chick reduces the number and width of myotubes and the number of MyoD positive-myoblasts.
Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides.
Nottingham, United Kingdom. In Proc Natl Acad Sci U S A, 05 Feb 2016
We showed that this GET (GAG-binding enhanced transduction) system could deliver enzymes (Cre, neomycin phosphotransferase), transcription factors (NANOG, MYOD), antibodies, native proteins (cytochrome C), magnetic nanoparticles (MNPs), and nucleic acids [plasmid (p)DNA, modified (mod)RNA, and small inhibitory RNA] at efficiencies of up to two orders of magnitude higher than previously reported in cell types considered hard to transduce, such as mouse embryonic stem cells (mESCs), human ESCs (hESCs), and induced pluripotent stem cells (hiPSCs).
Finding MyoD with a little help from my friends.
Boston, United States. In Nat Cell Biol, 2012
Findings indicate that MyoD activates the expression of other muscle regulators such as MEF2 and myogenin, which are necessary for induction of the skeletal muscle differentiation program.