In vivo identification of eugenol-responsive and muscone-responsive mouse odorant receptors.
Frankfurt am Main, Germany. In J Neurosci, 2014
Heterologous expression assays confirmed nine eugenol-responsive ORs (Olfr73, Olfr178, Olfr432, Olfr610, Olfr958, Olfr960, Olfr961, Olfr913, and Olfr1234) and four muscone-responsive ORs (Olfr74, Olfr235, Olfr816, and Olfr1440).
Ligand-selective activation of heterologously-expressed mammalian olfactory receptor.
Gainesville, United States. In Cell Calcium, 2014
To better understand the mechanisms and molecular range of such ligand selectivity, we expressed the mouse eugenol OR (mOR-EG) in HEK293T cells together with Gα15 to monitor activation of the phospholipase-C (PLC) signaling pathway and/or Gαolf to monitor activation of the adenylate cyclase (AC) signaling pathway, resulting in intracellular Ca(2+) release and/or Ca(2+) influx through a cyclic nucleotide-gated channel, respectively.
Ligand specificity of odorant receptors.
Trieste, Italy. In J Mol Model, 2007
Recent site-directed mutagenesis experiments on one such receptor (MOR174-9) provide information that helped to identify nine amino-acid residues involved in ligand binding.