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Myosin, light chain 12B, regulatory

MLC20
The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008] (from NCBI)
Top mentioned proteins: 20-kDa, Rhodopsin, myosin light chain kinase, V1a, RhoA
Papers on MLC20
A novel function of nuclear non-muscle myosin regulatory light chain in promotion of xanthine oxidase transcription after myocardial ischemia/reperfusion.
New
Peng et al., Changsha, China. In Free Radic Biol Med, 17 Mar 2015
UNASSIGNED: Nuclear myosin regulates gene transcription and this novel function might be modulated through phosphorylation of the myosin regulatory light chain (p-MLC20).
The importance of complete tissue homogenization for accurate stoichiometric measurement of myosin light chain phosphorylation in airway smooth muscle.
New
Seow et al., Vancouver, Canada. In Can J Physiol Pharmacol, 28 Feb 2015
The standard method for measuring the phosphorylation of the regulatory myosin light chain (MLC20) in smooth muscle is extraction of the light chain using a urea extraction buffer, urea-glycerol gel electrophoresis of the soluble portion of the extract (supernatant) and Western blot analysis.
Inhibition of RhoA-Dependent pathway and Contraction by Endogenous Hydrogen Sulfide in Rabbit Gastric Smooth Muscle Cells.
New
Murthy et al., Virginia, South Africa. In Am J Physiol Cell Physiol, 07 Feb 2015
The aim of this study was to analyse the expression of CSE and H2S function in the regulation of MLCP activity, MLC20 phosphorylation and contraction in isolated gastric smooth muscle cells.
VEGF receptors mediate hypoxic remodeling of adult ovine carotid arteries.
New
Pearce et al., Loma Linda, United States. In J Appl Physiol, Nov 2014
In arteries that were organ cultured 24 h with 3 nM VEGF, VEGF replicated effects of hypoxia on abundances of smooth muscle α actin (SMαA), myosin light chain kinase (MLCK), and MLC20 and the effects of hypoxia on colocalization of MLC20 with SMαA, as measured via confocal microscopy.
Regulation of contractile signaling and matrix remodeling by T-cadherin in vascular smooth muscle cells: constitutive and insulin-dependent effects.
New
Philippova et al., Basel, Switzerland. In Cell Signal, Sep 2014
Contractile machinery was constitutively altered in a manner indicative of reduced intrinsic contractile competence, namely decreased phosphorylation of MYPT1(thr696 or thr853) and MLC20(thr18/ser19), reduced RhoA activity and increased iNOS expression.
Hypercontractility of intestinal longitudinal smooth muscle induced by cytokines is mediated by the nuclear factor-κB/AMP-activated kinase/myosin light chain kinase pathway.
New
Murthy et al., Richmond, United States. In J Pharmacol Exp Ther, Jul 2014
In mouse colonic longitudinal muscle cells, acetylcholine (ACh) stimulated AMPK and MLCK phosphorylation and activity and induced MLC20 phosphorylation and muscle contraction.
Substance P is essential for maintaining gut muscle contractility: a novel role for coneurotransmission revealed by botulinum toxin.
New
Pasricha et al., Baltimore, United States. In Am J Physiol Gastrointest Liver Physiol, Jun 2014
At the desired time point, pylorus was dissected out and pyloric contractility was measured ex vivo in an organ bath and by measuring phosphorylation of myosin light chain 20 (MLC20).
The effect of DA-9701 on 5-hydroxytryptamine-induced contraction of feline esophageal smooth muscle cells.
Sohn et al., Seoul, South Korea. In Molecules, 2013
These G proteins activate PLC, which leads to Ca2+/calmodulin-dependent MLCK activation, resulting in MLC20 phosphorylation and cell contraction.
Perinatal nicotine exposure increases Angiotensin II receptor-mediated vascular contractility in adult offspring.
Zhang et al., Loma Linda, United States. In Plos One, 2013
KEY FINDINGS: Nicotine treatment enhanced Angitension II (Ang II)-induced vasoconstriction and 20-kDa myosin light chain phosphorylation (MLC20-P) levels.
Role of serine-threonine phosphoprotein phosphatases in smooth muscle contractility.
Review
Chan et al., Newcastle, Australia. In Am J Physiol Cell Physiol, 2013
The degree of phosphorylation of myosin light chain 20 (MLC20) is a major determinant of force generation in smooth muscle.
Blebbistatin, a myosin II inhibitor, suppresses Ca(2+)-induced and "sensitized"-contraction of skinned tracheal muscles from guinea pig.
Watanabe et al., Japan. In J Smooth Muscle Res, 2012
Blebbistatin, a potent inhibitor of myosin II, has inhibiting effects on Ca(2+)-induced contraction and contractile filament organization without affecting the Ca(2+)-sensitivity to the force and phosphorylation level of myosin regulatory light chain (MLC20) in skinned (cell membrane permeabilized) taenia cecum from the guinea pig (Watanabe et al., Am J Physiol Cell Physiol.
Myosin regulatory light chain diphosphorylation slows relaxation of arterial smooth muscle.
GeneRIF
Walsh et al., Calgary, Canada. In J Biol Chem, 2012
Findings conclude that phosphorylation at Thr(18) decreases the rates of LC(20) dephosphorylation and smooth muscle relaxation compared with LC(20) phosphorylated exclusively at Ser(19).
Phosphorylation of myosin II regulatory light chain controls its accumulation, not that of actin, at the contractile ring in HeLa cells.
GeneRIF
Hosoya et al., Hiroshima, Japan. In Exp Cell Res, 2012
phosphorylated MRLC temporally controls its own accumulation, but not that of actin, in cultured mammalian cells.
Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.
GeneRIF
Hosoya et al., Hiroshima, Japan. In Biochem Biophys Res Commun, 2012
These results suggest that 2P-MRLC has a role different from that of 1P-MRLC at the midzone, and is not a subunit of myosin II.
Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells.
GeneRIF
Egelhoff et al., Cleveland, United States. In Bmc Cell Biol, 2010
The study data show that manipulation of the activation sites (Thr18/Ser19) significantly alters myosin II function in a number of these assays while manipulation of the putative inhibitory sites (Ser1/Ser2/Thr9) does not.
Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
GeneRIF
Turck et al., São Paulo, Brazil. In Eur Arch Psychiatry Clin Neurosci, 2009
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
Role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, a review dedicated to Dr. Setsuro Ebashi.
Review
Kohama et al., Maebashi, Japan. In Biochem Biophys Res Commun, 2008
The kinase activity is mediated by the catalytic domain that phosphorylates the myosin light-chain of 20kDa (MLC20), activating smooth muscle myosin to interact with actin.
Sensitized airway smooth muscle plasticity and hyperreactivity: a review.
Review
Fust et al., Winnipeg, Canada. In Can J Physiol Pharmacol, 2007
Nevertheless, we did find that content and total activity of smooth muscle myosin light chain kinase (smMLCK) and the abundance of its message were greater; this would explain the increased MLC20 phosphorylation.
Signaling for contraction and relaxation in smooth muscle of the gut.
Review
Murthy, Richmond, United States. In Annu Rev Physiol, 2005
Phosphorylation of Ser19 on the 20-kDa regulatory light chain of myosin II (MLC20) by Ca2+/calmodulin-dependent myosin light-chain kinase (MLCK) is essential for initiation of smooth muscle contraction.
Signal-transduction pathways that regulate smooth muscle function I. Signal transduction in phasic (esophageal) and tonic (gastroesophageal sphincter) smooth muscles.
Review
Biancani et al., Providence, United States. In Am J Physiol Gastrointest Liver Physiol, 2005
The steps linking activation of PKC to myosin light chain (MLC20) phosphorylation and contraction, however, have not been clearly defined for LES, Eso, or other smooth muscles.
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