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Myosin, light chain 12B, regulatory

MLC20
The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008] (from NCBI)
Top mentioned proteins: 20-kDa, Rhodopsin, myosin light chain kinase, V1a, RhoA
Papers on MLC20
Role of non-MLC20 phosphorylation pathway in the regulation of vascular reactivity during shock.
New
Li et al., Chongqing, China. In J Surg Res, 30 Apr 2014
BACKGROUND: Studies have shown that shock-induced vascular hyporeactivity is associated with the decrease in 20-kDa myosin light chain (MLC20) phosphorylation.
PKC activation increases Ca2+ sensitivity of permeabilized lymphatic muscle via myosin light chain 20 phosphorylation-dependent and -independent mechanisms.
New
Muthuchamy et al., College Station, United States. In Am J Physiol Heart Circ Physiol, 31 Mar 2014
Western blot and immunohistochemistry data indicated that the initial rapid increase in tension development after stimulation by PDBu was associated with myosin light chain (MLC)20 phosphorylation; however, the later, steady-state Ca(2+) sensitization of permeabilized lymphatic muscle was not associated with increased phosphorylation of MLC20 at Ser(19), 17-kDa C-kinase-potentiated protein phosphatase-1 inhibitor at Thr(38), or caldesmon at Ser(789).
Inhibition of MLC20 phosphorylation downstream of Ca2+ and RhoA: A novel mechanism involving phosphorylation of myosin phosphatase interacting protein (M-RIP) by PKG and stimulation of MLC phosphatase activity.
New
Murthy et al., Richmond, United States. In Cell Biochem Biophys, Jan 2014
Treatment of permeabilized muscle cells with 10 μM Ca(2+) caused an increase in MLC20 phosphorylation and muscle contraction, but had no effect on Rho kinase activity.
Magnolol inhibits migration of vascular smooth muscle cells via cytoskeletal remodeling pathway to attenuate neointima formation.
New
Kim et al., Kansas City, United States. In Exp Cell Res, Jan 2014
The phosphorylation of myosin light chain (MLC20) was determined by urea-glycerol polyacrylamide gel electrophoresis.
Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis.
New
Wu et al., Changsha, China. In Plos One, Dec 2013
Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)-myosin light chain (MLC20), were analyzed using immunoblotting.
Differential regulation of muscarinic M2 and M3 receptor signaling in gastrointestinal smooth muscle by caveolin-1.
New
Murthy et al., Richmond, United States. In Am J Physiol Cell Physiol, Sep 2013
Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation.
Mitogen-activated protein kinases regulate vascular reactivity after hemorrhagic shock through myosin light chain phosphorylation pathway.
New
Liu et al., Chongqing, China. In J Trauma Acute Care Surg, Apr 2013
METHODS: With superior mesenteric arteries from hemorrhagic shock rats, the role of p38 MAPK, ERK, and JNK in the regulation of vascular reactivity following shock and their relationship to myosin light chain (MLC20) phosphorylation-dependent pathway was observed.
Role of serine-threonine phosphoprotein phosphatases in smooth muscle contractility.
Review
New
Chan et al., Newcastle, Australia. In Am J Physiol Cell Physiol, Mar 2013
The degree of phosphorylation of myosin light chain 20 (MLC20) is a major determinant of force generation in smooth muscle.
JunB mediates basal- and TGFβ1-induced smooth muscle cell contractility.
Adam et al., Boston, United States. In Plos One, 2012
However, JunB silencing decreased levels of Rho kinase (ROCK) and myosin light chain (MLC20).
Blebbistatin, a myosin II inhibitor, suppresses Ca(2+)-induced and "sensitized"-contraction of skinned tracheal muscles from guinea pig.
Watanabe et al., Japan. In J Smooth Muscle Res, 2012
Blebbistatin, a potent inhibitor of myosin II, has inhibiting effects on Ca(2+)-induced contraction and contractile filament organization without affecting the Ca(2+)-sensitivity to the force and phosphorylation level of myosin regulatory light chain (MLC20) in skinned (cell membrane permeabilized) taenia cecum from the guinea pig (Watanabe et al., Am J Physiol Cell Physiol.
Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA.
Murthy et al., Richmond, United States. In Plos One, 2012
PAR2-AP induced phosphorylation of MLC20, MYPT1 but not CPI-17.
Myosin regulatory light chain diphosphorylation slows relaxation of arterial smooth muscle.
GeneRIF
Walsh et al., Calgary, Canada. In J Biol Chem, 2012
Findings conclude that phosphorylation at Thr(18) decreases the rates of LC(20) dephosphorylation and smooth muscle relaxation compared with LC(20) phosphorylated exclusively at Ser(19).
Phosphorylation of myosin II regulatory light chain controls its accumulation, not that of actin, at the contractile ring in HeLa cells.
GeneRIF
Hosoya et al., Hiroshima, Japan. In Exp Cell Res, 2012
phosphorylated MRLC temporally controls its own accumulation, but not that of actin, in cultured mammalian cells.
Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.
GeneRIF
Hosoya et al., Hiroshima, Japan. In Biochem Biophys Res Commun, 2012
These results suggest that 2P-MRLC has a role different from that of 1P-MRLC at the midzone, and is not a subunit of myosin II.
Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells.
GeneRIF
Egelhoff et al., Cleveland, United States. In Bmc Cell Biol, 2010
The study data show that manipulation of the activation sites (Thr18/Ser19) significantly alters myosin II function in a number of these assays while manipulation of the putative inhibitory sites (Ser1/Ser2/Thr9) does not.
Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
GeneRIF
Turck et al., São Paulo, Brazil. In Eur Arch Psychiatry Clin Neurosci, 2009
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
Role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, a review dedicated to Dr. Setsuro Ebashi.
Review
Kohama et al., Maebashi, Japan. In Biochem Biophys Res Commun, 2008
The kinase activity is mediated by the catalytic domain that phosphorylates the myosin light-chain of 20kDa (MLC20), activating smooth muscle myosin to interact with actin.
Sensitized airway smooth muscle plasticity and hyperreactivity: a review.
Review
Fust et al., Winnipeg, Canada. In Can J Physiol Pharmacol, 2007
Nevertheless, we did find that content and total activity of smooth muscle myosin light chain kinase (smMLCK) and the abundance of its message were greater; this would explain the increased MLC20 phosphorylation.
Signaling for contraction and relaxation in smooth muscle of the gut.
Review
Murthy, Richmond, United States. In Annu Rev Physiol, 2005
Phosphorylation of Ser19 on the 20-kDa regulatory light chain of myosin II (MLC20) by Ca2+/calmodulin-dependent myosin light-chain kinase (MLCK) is essential for initiation of smooth muscle contraction.
Signal-transduction pathways that regulate smooth muscle function I. Signal transduction in phasic (esophageal) and tonic (gastroesophageal sphincter) smooth muscles.
Review
Biancani et al., Providence, United States. In Am J Physiol Gastrointest Liver Physiol, 2005
The steps linking activation of PKC to myosin light chain (MLC20) phosphorylation and contraction, however, have not been clearly defined for LES, Eso, or other smooth muscles.
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