A pRb-independent mechanism preserves the postmitotic state in terminally differentiated skeletal muscle cells
In The Journal of Cell Biology, 1996
... (BD Biosciences); p107, clone SD9; p130, C-20; cycD1, M-20; cycA, C-19; cycE, M-20; PCNA, PC10; MCM2, N-19 (Santa Cruz Biotechnology, Inc.); RPA70, 2H10 (NeoMarkers); ...
The mechanism of DNA replication termination in vertebrates.
Boston, United States. In Nature, Oct 2015
Dissociation of the replicative CMG helicase (comprising CDC45, MCM2-7 and GINS) occurs only after the final ligation step, and is not required for completion of DNA synthesis, strongly suggesting that converging CMGs pass one another and dissociate from double-stranded DNA.
Structure of the eukaryotic MCM complex at 3.8 Å.
Beijing, China. In Nature, Sep 2015
A central step in this replication is the assembly of the heterohexameric minichromosome maintenance (MCM2-7) helicase complex at replication origins during G1 phase as an inactive double hexamer.
Immunohistochemistry-based prognostic biomarkers in NSCLC: novel findings on the road to clinical use?
Uppsala, Sweden. In Expert Rev Mol Diagn, Apr 2015
Only 26 proteins first described after 2008 (ALDH1A1, ANXA1, BCAR1, CLDN1, EIF4E, EZH2, FOLR1, FOXM1, IL7R, IL12RB2, KIAA1524, CRMP1, LOX, MCM7, MTA1, MTDH, NCOA3, NDRG2, NEDD9, NES, PBK, PPM1D, SIRT1, SLC7A5, SQSTM1 and WNT1) demonstrated a consistent prognostic association in two or more independent patient cohorts, thus qualifying as promising candidates for diagnostic use.
Crystal structure of the eukaryotic origin recognition complex.
Baltimore, United States. In Nature, Apr 2015
Comparative analyses indicate that ORC encircles DNA, using its winged-helix domain face to engage the mini-chromosome maintenance 2-7 (MCM2-7) complex during replicative helicase loading; however, an observed out-of-plane rotation of more than 90° for the Orc1 AAA+ domain disrupts interactions with catalytic amino acids in Orc4, narrowing and sealing off entry into the central channel.
Helicase loading: how to build a MCM2-7 double-hexamer.
London, United Kingdom. In Semin Cell Dev Biol, 2014
A central step in eukaryotic initiation of DNA replication is the loading of the helicase at replication origins, misregulation of this reaction leads to DNA damage and genome instability.
Viral and cellular biomarkers in the diagnosis of cervical intraepithelial neoplasia and cancer.
Napoli, Italy. In Biomed Res Int, 2012
This review will focus on the analysis of clinical validated or candidate biomarkers, such as HPV DNA, HPV E6/E7 mRNA, HPV proteins, p16(INK4a) and Ki67, TOP2A and MCM2 cellular factors, and DNA methylation profiles, which will likely improve the identification of premalignant lesions that have a high risk to evolve into invasive cervical cancer.