Higher-intensity exercise helps cancer survivors remain motivated.
Seaside, United States. In J Cancer Surviv, Dec 2015
Intervention participants were randomized to low-to-moderate intensity exercise (LIG; n = 44; 60-65 % VO2peak, 50-65 % one repetition maximum (1RM)) or moderate-to-high intensity exercise (HIG; n = 40; 75-80 % VO2peak, 65-80 % 1RM) while controls continued usual care.
Preparation of sumoylated substrates for biochemical analysis.
Boston, United States. In Methods Mol Biol, 2008
Here, we describe the purification of the ubiquitin conjugating enzyme E2-25K, its in vitro sumoylation with recombinant enzymes and the subsequent separation and purification of the modified and the unmodified forms.
Sequential E2s drive polyubiquitin chain assembly on APC targets.
San Francisco, United States. In Cell, 2007
The anaphase-promoting complex (APC), or cyclosome, is an E3 ubiquitin-protein ligase that collaborates with E2 ubiquitin-conjugating enzymes to assemble polyubiquitin chains on proteins important for cell-cycle progression.