Flow cytometric assays for the study of autophagy.
London, United Kingdom. In Methods, Aug 2015
These include the detection of the autophagy marker, the microtubule associated protein LC3B, cell cycle analysis of LC3B expression, increase in lysosomal mass, as well as organelle specific autophagy and the measurement of mitochondrial function.
Dual function of CALCOCO2/NDP52 during xenophagy.
More papers using
Lyon, France. In Autophagy, 2014
Indeed, to mediate autophagosome maturation CALCOCO2 binds on the one hand to LC3A, LC3B, or GABARAPL2, and on the other hand to MYO6/MYOSIN VI, whereas the interaction with LC3C is dispensable.
Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems.
In PLoS Pathogens, 2000
... (16B12, Covance Inc.), HA (3F10, Roche), NF-κB p65 (F-6, Santa Cruz, or C22B4, Cell Signaling), LC3B (D11, Cell signaling), IE1 antibody (CROMA101; provided ... Tumor suppressor loci on mouse chromosomes 9 and 16 are lost at distinct stages of tumorigenesis in a transgenic model of islet cell carcinoma
In Cell Death & Disease, 1994
... Signaling Technology), anti-Caspase-9 (1:500, #9504, Cell Signaling Technology), anti-Cleaved Caspase-9 (1:250, #9509, Cell Signaling Technology), anti-LC3B (1:500, ab48394, Abcam, Cambridge, UK), anti-HIF-1 ...